| Ektc Group Ltd | Information & communication | 2025 |
| EL Safrejoe Limited | Information & communication | 2015 |
| Elburritomonster Ltd | Information & communication | 2022 |
| Electio Partners Ltd | Information & communication | 2018 |
| Electrical Power Systems Limited | Professional & scientific | 1992 |
| Electromend Ltd | Other services | 2019 |
| Eleged Limited | Wholesale & retail | 2004 |
| Elephant Foot Limited | Financial services | 2022 |
| ELEV8EXEC Ltd | Professional & scientific | 2023 |
| Elevate Innovation Ltd | Wholesale & retail | 2019 |
| Eleven Salon Ltd | Other services | 2020 |
| ELF Mill Kitchen Limited | Hospitality | 2022 |
| Elissa ELH Ltd | Health & social care | 2021 |
| Elite Global Talent (UK) Limited | Administrative & support | 2019 |
| Elite Precise Trading Co. Ltd | Wholesale & retail | 2020 |
| Elite Projects NW Ltd | Construction | 2020 |
| Elite Property Evolution Ltd | Administrative & support | 2016 |
| Elite Sports Medicine Limited | Health & social care | 2025 |
| Eliza Theodore Ltd | Real estate | 2021 |
| Elkwood Construction Limited | Other services | 2015 |
| Elle Sera Ltd | Wholesale & retail | 2020 |
| Elle UK Limited | Manufacturing | 2003 |
| Ellis Flooring Ltd | Construction | 2022 |
| ELM Reports Limited | Health & social care | 2023 |
| Elmsthorpe Gardens Management Company Limited | Households | 2014 |
| Elphick Properties Limited | Real estate | 2019 |
| ELR Locum Limited | Health & social care | 2013 |
| Elro Medical Limited | Health & social care | 2025 |
| Elusive Luxury Limited | Wholesale & retail | 2020 |
| Elvara Holdings Ltd | Real estate | 2026 |
| Elygra Limited | Professional & scientific | 2003 |
| Elygra Services Ltd | Administrative & support | 2023 |
| Elysium Aesthetics Health and Wellbeing LLP | | 2019 |
| ELZ Ltd | Health & social care | 2019 |
| ELZ Property Holdings Limited | Real estate | 2021 |
| EM Cheshire Consulting Limited | Administrative & support | 2013 |
| Emba Radiology Ltd | Health & social care | 2023 |
| Emcue Ltd | Information & communication | 2023 |
| Emda Limited | Health & social care | 2010 |
| Emerald Capital Limited | Construction | 2020 |
| Emidor Limited | Health & social care | 2014 |
| Emily Monk Education Limited | Education | 2021 |
| Emily Monk Hairdressing Limited | Other services | 2021 |
| Emily Monk Products Limited | Wholesale & retail | 2021 |
| EML Realisations Limited | Manufacturing | 1976 |
| EMM Living Spaces Ltd | Administrative & support | 2019 |
| Emmett Enterprises Limited | Wholesale & retail | 2026 |
| Empathy Homes Ltd | Construction | 2025 |
| Empathy Housing Ltd | Real estate | 2022 |
| Emphasys Limited | Information & communication | 2001 |
| Empire Fine Jewellers Ltd | Wholesale & retail | 2021 |
| Empire Global Security Ltd | Administrative & support | 2020 |
| Empiria Ltd | Information & communication | 2000 |
| Employment Law Advisory Services Limited | Extraterritorial | 1995 |
| Empower-Autism CIC | Professional & scientific | 2025 |
| EMW Medical Limited | Health & social care | 2019 |
| Endcliffe Oncology Ltd | Health & social care | 2022 |
| Endo (Cheshire) Limited | Health & social care | 2010 |
| Endo Values Limited | Health & social care | 2022 |
| Endon Associates Limited | Transport & logistics | 2009 |
| Endurance Sports Medicine Ltd | Health & social care | 2011 |
| Energy Efficiency Surveys Ltd | Professional & scientific | 2014 |
| Energy Rigging Ltd | Professional & scientific | 2026 |
| Engage Coach International Ltd | Professional & scientific | 2016 |
| Engineering and Welding Consultants Limited | Professional & scientific | 2003 |
| English Homes Limited | Construction | 1992 |
| English Land Limited | Construction | 1993 |
| Ensco 2019 Limited | Real estate | 2019 |
| ENT Centre of Excellence LLP | | 2024 |
| Entech Farms Limited | Professional & scientific | 2018 |
| Entirespace Limited | Real estate | 2007 |
| Environmental Tax Advisory Ltd | Professional & scientific | 2019 |
| Envoy Labs Limited | Information & communication | 2020 |
| Epastandard Ltd | Hospitality | 2025 |
| Epic Leading Ltd | Professional & scientific | 2017 |
| Epicure Foods Limited | Wholesale & retail | 2018 |
| Eplatform Solutions Ltd | Information & communication | 2006 |
| EPS3 Limited | Real estate | 2026 |
| Equilibrium Financial Planning Holdco Ltd | Financial services | 2025 |
| Equilibrium Financial Planning LLP | | 2005 |
| Equilibrium Healthcare Group Limited | Health & social care | 2006 |
| Equilibrium Healthcare Limited | Health & social care | 1996 |
| Equilibrium Investment Management Holdco Ltd | Financial services | 2025 |
| Equilibrium Investment Management LLP | | 2014 |
| Equipath Ltd | Financial services | 2026 |
| Ergolife Group Limited | Financial services | 2018 |
| Erox 23 Ltd | Other services | 2024 |
| ERP Experts (Europe) Limited | Information & communication | 2016 |
| ERS Ross Limited | Health & social care | 2016 |
| ERT Ents Limited | Administrative & support | 2026 |
| ESC Schools Ltd | Education | 2020 |
| ESG Recruitment Limited | Administrative & support | 2015 |
| Esgmark Limited | Professional & scientific | 2020 |
| ESI Technical Services Limited | Professional & scientific | 2024 |
| Esito Limited | Professional & scientific | 2011 |
| Esmk Services Ltd | Health & social care | 2014 |
| Espee Design & Development Limited | Professional & scientific | 2006 |
| Esscenti Ltd | Wholesale & retail | 2018 |
| Essency Consulting Limited | Information & communication | 2004 |
| Estate Property Management Limited | Real estate | 2025 |