| Drive UP Storage Widnes Ltd | Administrative & support | 1983 |
| DRL Civil Building & Maintenance Ltd | Construction | 2019 |
| Drymatter Sales Ltd | Other services | 2022 |
| DS Heating Systems Ltd | Construction | 2021 |
| DS Property North West Ltd | Real estate | 2025 |
| DSO Building Services Ltd | Construction | 2020 |
| DSO Joinery Ltd | Construction | 2026 |
| DSO Roofing Services Limited | Construction | 2024 |
| DT2 Autos Ltd | Wholesale & retail | 2025 |
| DTR Vehicle Tuning Ltd | Wholesale & retail | 2020 |
| Dumitru Razvan Logistic Limited | Transport & logistics | 2018 |
| Duncan Sexton Limited | Wholesale & retail | 1973 |
| Dund Performance Group Ltd | Professional & scientific | 2024 |
| Dunning Futures Limited | Energy | 2022 |
| Durlston Services Ltd | Construction | 2019 |
| Dutch Barrel Limited | Hospitality | 2024 |
| DVR Plumbing & Heating Ltd | Administrative & support | 2017 |
| DWC Consulting Limited | Professional & scientific | 2025 |
| Dyffryn Memorials Limited | Other services | 2024 |
| Dylan Jake Properties Ltd | Real estate | 2020 |
| Dynam Hand Car Wash and Valenting Ltd | Administrative & support | 2025 |
| Dynamic Housing & Support Ltd | Hospitality | 2024 |
| E & a Electrical Services Ltd | Construction | 2000 |
| E & a Joinery Ltd | Construction | 2014 |
| E & P Catterall Ltd | Construction | 2017 |
| E a Engineering (North West) Limited | Construction | 2021 |
| E E Installations Ltd | Manufacturing | 2019 |
| E S E Consultants Ltd | Manufacturing | 2015 |
| E S E Group Limited | Administrative & support | 2019 |
| E W G Haulage Ltd | Transport & logistics | 2025 |
| E&M Energy Consultancy Ltd | Professional & scientific | 2024 |
| E-Go Logistics Ltd | Transport & logistics | 2023 |
| E-Tech Components (UK) Limited | Wholesale & retail | 2004 |
| E.O.G. Engineered Solutions Ltd | Other services | 2017 |
| EA & L Properties Limited | Real estate | 2025 |
| Eagle EYE Medical Imaging Ltd | Health & social care | 2025 |
| Easy Vehicle Lease Ltd | Administrative & support | 2017 |
| Eatmate Ltd | Wholesale & retail | 2022 |
| Ec&I Solutions UK Ltd | Construction | 2021 |
| Echelon Coffee Group Limited | Administrative & support | 2004 |
| Echo Electrical (NW) Ltd | Construction | 2026 |
| Eco Energy Cleaning Solutions Limited | Administrative & support | 2010 |
| Ecoshred Limited | Other services | 2010 |
| Ecovida Routes Ltd | Professional & scientific | 2023 |
| Eden Healthcare Services (NW) Limited | Health & social care | 2018 |
| Edward Medical Limited | Administrative & support | 2026 |
| Edwards Clothes Ltd | Wholesale & retail | 2025 |
| Edwards Landscapes Limited | Water & waste | 2021 |
| EF Software Developments Limited | Information & communication | 2019 |
| Efes Mezze Ltd | Hospitality | 2026 |
| Egerton Property Group Northwest Ltd | Real estate | 2025 |
| EI7 Ltd | Transport & logistics | 2024 |
| EL Rose Silk Flower Boutique Ltd | Wholesale & retail | 2023 |
| Elbon (U.K.) Limited | Wholesale & retail | 2016 |
| Electra Services Ltd | Construction | 2025 |
| Electrical Safety Inspection Services Limited | Manufacturing | 2005 |
| Elegance Travel Ltd | Transport & logistics | 2019 |
| Elegant Living Wholesale Limited | Wholesale & retail | 2025 |
| Element Communications & Design Limited | Administrative & support | 2005 |
| Elevate Padel Ltd | Extraterritorial | 2025 |
| Elevatedelectrics Ltd | Manufacturing | 2024 |
| Elite Autofix Ltd | Wholesale & retail | 2010 |
| Elite Bars Cheshire Limited | Hospitality | 2018 |
| Elite Employment Solutions Limited | Administrative & support | 2014 |
| Elite Estates North West Limited | Real estate | 2024 |
| Elite Logistics & Transport Recruitment Limited | Administrative & support | 2025 |
| Elite Properties NW Limited | Real estate | 2018 |
| Elite Residential Properties (NW) Limited | Real estate | 2021 |
| Elleroseuk Ltd | Manufacturing | 2026 |
| Elliots Construction Ltd | Construction | 2013 |
| Elliott O'Hares Barber Shops Limited | Other services | 2014 |
| Ellsmoor Holdings Ltd | Financial services | 2025 |
| Elluminet Press Ltd | Information & communication | 2018 |
| Elmac Consulting Limited | Professional & scientific | 2015 |
| Eltham Walk Management Co Limited | Households | 2019 |
| Emarel Civil Engineering Limited | Extraterritorial | 2012 |
| Emben Holdings Limited | Financial services | 2016 |
| Embrace Orthodontics Limited | Health & social care | 2018 |
| Emcas Financial Reporting Limited | Education | 2017 |
| EMH (UK) Ltd | Professional & scientific | 2010 |
| Emis Safety Ltd | Professional & scientific | 2023 |
| Emos Retail Ltd | Wholesale & retail | 2025 |
| Empire Investment Holdings Limited | Real estate | 2024 |
| Empower ME Through RSE Ltd | Education | 2021 |
| Emris Services Ltd | Administrative & support | 2018 |
| Enee.Io Ltd | Information & communication | 2019 |
| Energy Property Services (EPS) Ltd | Construction | 2009 |
| Engrave Hub Ltd | Manufacturing | 2024 |
| Enigma Sport Ltd | Education | 2023 |
| Enjay Joinery & Construction Limited | Construction | 2007 |
| Enrec Ltd | Construction | 2024 |
| Enthusiasm Developments Limited | Construction | 2019 |
| Entrepreneur Limited | Information & communication | 2005 |
| Entroware Ltd | Manufacturing | 2024 |
| Enviro Clean Merseyside Ltd | Other services | 2021 |
| Environmental Options Group Ltd | Professional & scientific | 2007 |
| EP Auto Techs Ltd | Professional & scientific | 2023 |
| EP Estates Limited | Real estate | 2015 |
| Epic Event Extras Ltd | Administrative & support | 2024 |
| Equity Private Clients Ltd | Professional & scientific | 2009 |