| Electcatch Limited | Agriculture & fishing | 1983 |
| ELF Marketing Ltd | Professional & scientific | 2013 |
| Elite Joinery Whitby Ltd | Construction | 2025 |
| Ellabbey Ltd | Real estate | 2019 |
| Elliott Consultancy Limited | Professional & scientific | 2005 |
| Emma Stothard Sculpture Ltd | Arts & recreation | 2018 |
| Emma's Diner Ltd | Hospitality | 2024 |
| Emmott Property Ltd | Real estate | 2026 |
| Empire Scaffolding Whitby Ltd | Construction | 2021 |
| Endeavour House Management Limited | Administrative & support | 1998 |
| Engensia Design Limited | Information & communication | 2015 |
| ER Cornforth Ltd | Agriculture & fishing | 2020 |
| Esho Funi Limited | Wholesale & retail | 2006 |
| ESK Audio Limited | Arts & recreation | 2011 |
| ESK Moors Active Ltd | Transport & logistics | 2005 |
| ESK Moors Caring Ltd | Health & social care | 2005 |
| ESK Valley Environmental Ltd | Professional & scientific | 2014 |
| ESK Valley Properties Limited | Real estate | 2023 |
| ESK Valley Property Holdings Limited | Real estate | 2026 |
| ESK Valley Racing Limited | | 1981 |
| ESK Valley Theatre | Arts & recreation | 2008 |
| Eskdale Agricultural Engineering Co. Limited | Real estate | 1964 |
| Eskdale Community Trust for Education Limited | Education | 1978 |
| Eskdale Fisheries Ltd | Hospitality | 2017 |
| Eskdale Forestry Limited | Agriculture & fishing | 2019 |
| Eskdale Stone Limited | Mining & quarrying | 1996 |
| Esplanade Hotel Limited | Hospitality | 2018 |
| Essex Locomotive Society Limited (the) | Manufacturing | 1973 |
| Estambul Group Ltd | Manufacturing | 2023 |
| Europa-Technia Limited | Manufacturing | 1994 |
| Evol Trading Limited | Financial services | 2014 |
| Expert Clinical Services Limited | Health & social care | 2025 |
| Exquisite Luxury Ltd | Manufacturing | 2017 |
| Eyemazing Whitby Ltd | Professional & scientific | 2022 |
| Fairhaven York Limited | Financial services | 2002 |
| Fairhead Gundogs Ltd | Agriculture & fishing | 2026 |
| Falling Foss Limited | Hospitality | 2014 |
| Farmilo Holidays Limited | Hospitality | 2010 |
| Farsyde Limited | Hospitality | 2024 |
| FAT Albert's Bakehouse Ltd | Hospitality | 2026 |
| Fayvan Apartments Limited | Real estate | 2005 |
| Feeding and Sling Support Whitby CIC | Health & social care | 2022 |
| Fern KBB Limited | Construction | 2024 |
| Fern Leisure Ltd | Construction | 2026 |
| Figaro Pizza Ltd | Hospitality | 2023 |
| Finance JO Ltd | Professional & scientific | 2024 |
| Find Your Calm Ltd | Health & social care | 2020 |
| Finesttimber Ltd | Wholesale & retail | 2024 |
| Finkle Forge Ltd | Manufacturing | 2024 |
| First in Leisure Limited | Hospitality | 2012 |
| First Wave Employment Ltd | Administrative & support | 2022 |
| Flame Force Agency Ltd | Information & communication | 2025 |
| Flames Grill Whitby N Ltd | Hospitality | 2025 |
| Flash Company Arts Limited CIC | Arts & recreation | 2018 |
| Flat 1 Seabreeze Ltd | Hospitality | 2020 |
| Flatfortyseven Ltd | Real estate | 2018 |
| Fletcher & Woodhouse Limited | Wholesale & retail | 2005 |
| Flock Kitchen Ltd | Hospitality | 2024 |
| Flow Medical Communications Ltd | Professional & scientific | 2024 |
| Flow Swim Ltd | Arts & recreation | 2024 |
| Flowergate Gallery Ltd | Wholesale & retail | 2009 |
| Fontenoy Properties Ltd | Real estate | 2020 |
| Footcheck Ltd | Health & social care | 2024 |
| Forever to Hold Limited | Wholesale & retail | 2017 |
| Forgewell Partners Ltd | Administrative & support | 2025 |
| Forjosef Ltd | Professional & scientific | 2026 |
| Foss Escapes Ltd | Hospitality | 2024 |
| Fowle Green Management Ltd | Real estate | 2020 |
| FOX and Hounds Goldsborough Ltd | Hospitality | 2018 |
| Franco's Italian Cakes Limited | Wholesale & retail | 2024 |
| Fresh CUT Chips Ltd | Manufacturing | 2024 |
| Friendly Machine Ltd | Information & communication | 2020 |
| Friends of Whitby Abbey | Information & communication | 1989 |
| Frontline (East) Ltd | Professional & scientific | 2025 |
| Fryup Forge Steel Building Solutions Ltd | Manufacturing | 2018 |
| Fryup Shepherds Huts Ltd | Wholesale & retail | 2022 |
| Fudge's Taxis Limited | Transport & logistics | 2020 |
| Fusco (Holdings) Ltd | Financial services | 2017 |
| Fyling Hall (Yorkshire) Limited | Real estate | 1963 |
| Fyling Park Minerals Limited | Real estate | 2012 |
| Fylingdales Letting Services Limited | Real estate | 2023 |
| G Wright Plumbing & Heating Services Limited | Construction | 2023 |
| G&S Engineering Ltd | Professional & scientific | 2022 |
| G'S Bar & Kitchen Limited | Hospitality | 2026 |
| G-Tec Rope Access Ltd | Construction | 2024 |
| G. Collinson (Construction) Limited | Hospitality | 1991 |
| Ganister Ltd | Professional & scientific | 2012 |
| Garry Tuby Ltd | Wholesale & retail | 2017 |
| GCC Asset Management Ltd | Hospitality | 2020 |
| GCM (Whitby) Limited | Manufacturing | 2021 |
| Gembor Ltd | Real estate | 2010 |
| GET a Grip (Whitby) Ltd | Wholesale & retail | 2025 |
| Getaway Travel Service Limited | Administrative & support | 2024 |
| Ghosts of Whitby Ltd | Wholesale & retail | 2026 |
| Ghyll Wood Developments Limited | Construction | 1996 |
| Gibson Solutions Limited | Professional & scientific | 2010 |
| Giftcard Consulting Ltd | Professional & scientific | 2000 |
| Ginos Whitby Ltd | Hospitality | 2019 |
| Giorgio's of Whitby Ltd | Hospitality | 2018 |
| Glenlawn Properties Limited | Real estate | 1965 |