| Street House Championships Limited | Arts & recreation | 2024 |
| Stu-Art Creative Limited | Other services | 2010 |
| Studio 7 Tanning Rooms Limited | Other services | 2023 |
| Studio Sixty Ltd | Other services | 2022 |
| Styles Radiology Ltd | Professional & scientific | 2025 |
| Substantial Bidco Limited | Financial services | 2020 |
| Substantial Group Limited | Information & communication | 2019 |
| Substantial Holdco Limited | Financial services | 2024 |
| Substantial Manco Limited | Professional & scientific | 2025 |
| Substantial Midco 2 Limited | Financial services | 2021 |
| Substantial Midco Limited | Financial services | 2020 |
| Substantial Topco Limited | Financial services | 2020 |
| SUE Petri Consulting Limited | Professional & scientific | 2018 |
| Suffolks PUB Company Ltd | Hospitality | 2022 |
| Sugars Fast Food & Kitchen Wholesale Ltd | Hospitality | 2021 |
| Sugars Royale Ltd | Hospitality | 2026 |
| Supply Chain Progress Ltd | Professional & scientific | 2014 |
| Surman & Croome PMC Ltd | Administrative & support | 2017 |
| Surman Holdings Limited | Professional & scientific | 2008 |
| SWB Business Solutions Limited | Professional & scientific | 2012 |
| Sweetpea Pets Ltd | Wholesale & retail | 2017 |
| Sweetpea's Paws Ltd | Wholesale & retail | 2021 |
| Symbotics Limited | Information & communication | 1997 |
| Synthesis Electrical & Data Installations Limited | Information & communication | 2015 |
| T.M. Reeves Farm Machinery Limited | Wholesale & retail | 2012 |
| T2 Alloys Limited | Wholesale & retail | 2018 |
| T2 International Ltd | Professional & scientific | 2019 |
| T5 Engineering Ltd | Administrative & support | 2018 |
| Tableware Distribution Limited | Wholesale & retail | 2007 |
| Tabsa Beauty Ltd | Other services | 2022 |
| Tactical Marketing Solutions Ltd | Professional & scientific | 2019 |
| Tandata Consulting Ltd | Information & communication | 2016 |
| Tannery Court Management (Tewkesbury) Limited | Administrative & support | 2015 |
| TAP & ZAP Ltd | Extraterritorial | 2018 |
| Target Active Fitness Limited | Arts & recreation | 2016 |
| Taylor Farm Supplies Ltd | Agriculture & fishing | 2015 |
| Taylormade Services Cheltenham Limited | Administrative & support | 2016 |
| Taylor’S Kitchen Ltd | Hospitality | 2025 |
| TCG - IT Limited | Information & communication | 2007 |
| TCH2021 Ltd | Hospitality | 2021 |
| TD360 Ltd | Professional & scientific | 2026 |
| Teal Products Limited | Manufacturing | 2000 |
| Team Marketing Solutions Limited | Professional & scientific | 2017 |
| Team Police Ltd | Administrative & support | 2020 |
| Teiken Global Ltd | Professional & scientific | 2022 |
| Tenant Ready Property Services Ltd | Administrative & support | 2019 |
| Tender Leaves Ltd | Wholesale & retail | 2023 |
| Teqshop Ltd | Wholesale & retail | 2024 |
| Tern Marketing Ltd | Professional & scientific | 2024 |
| Terra Albion Ltd | Professional & scientific | 2018 |
| Terry Jeynes SKI Coaching Ltd | Education | 2023 |
| Tewkesbury Abbey Limited | Wholesale & retail | 1992 |
| Tewkesbury Clouds Ltd | Wholesale & retail | 2023 |
| Tewkesbury Electrical Limited | Construction | 2021 |
| Tewkesbury Fryer (Walton Cardiff) Ltd | Hospitality | 2024 |
| Tewkesbury Glass Limited | Extraterritorial | 2013 |
| Tewkesbury Indian Cuisine Limited | Hospitality | 2021 |
| Tewkesbury Indian Limited | Hospitality | 2026 |
| Tewkesbury Marina Limited(The) | Other services | 1969 |
| Tewkesbury Masonic Hall Limited | Health & social care | 1964 |
| Tewkesbury MOT Centre Limited | Wholesale & retail | 2010 |
| Tewkesbury Motorcycles Ltd | Wholesale & retail | 2026 |
| Tewkesbury Park Limited | Hospitality | 2014 |
| Tewkesbury Printing Co. Limited | Manufacturing | 1990 |
| Tewkesbury Rugby Football Club Limited | Arts & recreation | 1983 |
| Tewkesbury Town Colts Sports Club CIC | Arts & recreation | 2025 |
| Tewkesbury's Hand Car Wash Limited | Other services | 2014 |
| Thcw Ltd | Administrative & support | 2025 |
| THE Artisan Apiary Ltd | Wholesale & retail | 2024 |
| THE Association of Veterinary Animal Care Assistants Limited | Other services | 2021 |
| THE Beauty Hack Limited | Wholesale & retail | 2025 |
| THE Beckford Collective Ltd | Wholesale & retail | 2022 |
| THE Business Growth Network Ltd | Administrative & support | 2018 |
| THE Buy to LET Calculator Company Ltd | Information & communication | 2025 |
| THE Canine Group Ltd | Education | 2024 |
| THE Carousel Tearooms Ltd | Hospitality | 2025 |
| THE Chancery Media Ltd | Information & communication | 2026 |
| THE Cheltenham Publishing House Ltd | Extraterritorial | 2025 |
| THE Crescent Veterinary Centre Ltd | Professional & scientific | 2019 |
| THE Dentist @ Tupsley Ltd | Health & social care | 2022 |
| THE FAB Shack Ltd | Wholesale & retail | 2019 |
| THE Fastener Warehouse Limited | Manufacturing | 2013 |
| THE Front Runner's Network Limited | Other services | 2022 |
| THE Geek Shack Ltd | Manufacturing | 2015 |
| THE Genesis Club Ltd | Arts & recreation | 2025 |
| THE Hide Coffee and Tea House Ltd | Hospitality | 2019 |
| THE Ice Cream Cottage Ltd | Hospitality | 2025 |
| THE Innovation Hive Limited | Professional & scientific | 2024 |
| THE KIT BOY Ltd | Wholesale & retail | 2016 |
| THE Ledger Centre Limited | Professional & scientific | 2013 |
| THE Marketing Piece Ltd | Professional & scientific | 2023 |
| THE Mindset in Motion Psych Ltd | Health & social care | 2026 |
| THE Noise Chap Ltd. | Health & social care | 2004 |
| THE Northcote Handyman Ltd | Construction | 2017 |
| THE Old Orchard Bredon Management Company Limited | Households | 2017 |
| THE One Bait Ltd | Agriculture & fishing | 2025 |
| THE Publishing Studio Ltd | Information & communication | 2023 |
| THE Quaffing Cuisine PUB Company Limited | Hospitality | 2023 |
| THE Railway Inn @ Ripple Limited | Hospitality | 2015 |
| THE Real Property Collective Ltd | Real estate | 2024 |