| 1234 Properties Ltd | Real estate | 2021 |
| 1ST Choice Aerial & Satellites Ltd | Construction | 2014 |
| 24 Hour Open Studio Limited | Professional & scientific | 1990 |
| 27 Dartmouth Road Flats Management Limited | Households | 1990 |
| 3C Legal Limited | Professional & scientific | 2017 |
| 3CT Security Limited | Information & communication | 2022 |
| 3DXPRINTLY Ltd | Wholesale & retail | 2025 |
| 3LB Land Ltd | Real estate | 2016 |
| 4BMC Limited | Real estate | 2007 |
| 4J Property Limited | Real estate | 2021 |
| 4TL Ltd | Transport & logistics | 2022 |
| 808 Contracting Ltd | Transport & logistics | 2024 |
| 808S and Jacob Ltd | Wholesale & retail | 2026 |
| 9 Park Place (Cheltenham) Management Company Limited | Real estate | 1993 |
| 98 Church Street Residents Limited | Households | 1990 |
| 9C Networks Limited | Information & communication | 2015 |
| A & D Fine Finishing Ltd | Construction | 2025 |
| A & M Alldrick Ltd | Professional & scientific | 2022 |
| A & P Hiorns Construction Ltd | Construction | 2020 |
| A Bloom Financial Services Ltd | Financial services | 2022 |
| A Class of Beauty Limited | Other services | 2022 |
| A Cotswold Lifestyle Ltd | Wholesale & retail | 2021 |
| A D S Design & Brand Development Limited | Administrative & support | 1999 |
| A D Woodley Holdings 2021 Limited | Financial services | 2021 |
| A G B Renewables Ltd | Construction | 2012 |
| A G Boniface & Sons Limited | Construction | 2002 |
| A Large Holdings Ltd | Wholesale & retail | 2022 |
| A N P Holdings Limited | Financial services | 2021 |
| A Rhodes-Smith Ltd | Construction | 2022 |
| A Sound Mind Ltd | Other services | 2015 |
| A to Z Talent Solutions Ltd | Administrative & support | 2024 |
| A.J.Woodward & Sons Limited | Hospitality | 1925 |
| A2AYPORT Ltd | Transport & logistics | 2024 |
| A40 Stone and Sand Suppliers Ltd | Wholesale & retail | 2020 |
| Aaron Apperley Property Ltd | Construction | 2019 |
| AB1 Investments Ltd | Financial services | 2015 |
| Abacus Money Management Limited | Financial services | 2008 |
| Abbey Business Consultancy Limited | Professional & scientific | 2013 |
| Abbey Court (Tewkesbury) Residents Association Limited | Households | 1997 |
| Abbey Electrical Ltd | Construction | 2026 |
| Abbey Mill (Tewkesbury) Management Limited | Extraterritorial | 2005 |
| Abbeyfield Tewkesbury Society Ltd | Hospitality | 1967 |
| Abec (Group) Ltd | Financial services | 2021 |
| Abec (ME) Ltd | Manufacturing | 2021 |
| Abec Ltd | Manufacturing | 2007 |
| ABI Engineering Limited | Manufacturing | 2005 |
| ABLE2 Wear Limited | Manufacturing | 2017 |
| Accommodation Services (Holdings) Limited | Construction | 1999 |
| Ace Filters Ltd | Wholesale & retail | 2022 |
| Achievable Care Quality Community Ltd | Administrative & support | 2024 |
| Achillea Wellness Ltd | Wholesale & retail | 2024 |
| Acoem UK Ltd | Professional & scientific | 2000 |
| Acorn BID Co Limited | Financial services | 2025 |
| Acorn Top Co Limited | Financial services | 2025 |
| ACS Fabrications Ltd | Manufacturing | 2024 |
| Active Trade Frames Ltd | Manufacturing | 2018 |
| Acupuncture Training Ltd | Administrative & support | 2019 |
| Adam Barber Shop GL20 Ltd | Other services | 2026 |
| Adam Harper Boxing Clever CIC | Education | 2022 |
| Adamant Club Ltd | Wholesale & retail | 2025 |
| Adams Glass and Glazing Limited | Construction | 2026 |
| Adapt-It Limited | Information & communication | 2001 |
| Addyson Monitoring Services Limited | Professional & scientific | 2010 |
| Adeptus Radiologica Ltd | Professional & scientific | 2024 |
| Adglow (Installations) Limited | Manufacturing | 2010 |
| Adglow Limited | Wholesale & retail | 1957 |
| ADI QA Services Ltd | Information & communication | 2024 |
| ADS Design and Brand Development Holdings Limited | Professional & scientific | 2000 |
| Advice 4U Financial Planning Ltd | Financial services | 2015 |
| Afrodites Team Limited | Hospitality | 2026 |
| AGP Autoservice Ltd | Wholesale & retail | 2019 |
| Agriservices (Gloshire) Limited | Construction | 1985 |
| AH123 Limited | Professional & scientific | 2024 |
| Airsearch Ltd | Administrative & support | 2020 |
| Airsun Systems Limited | Construction | 2013 |
| Aitken Engineering Services Ltd | Professional & scientific | 2018 |
| AJ Developments (Bredon) Ltd | Real estate | 2020 |
| AJK Commercial Consultancy Limited | Professional & scientific | 2003 |
| AK & Sons (Gloucester) Ltd | Construction | 2011 |
| ALB Properties (SW) Ltd | Real estate | 2025 |
| Alderton Community Trust | Administrative & support | 2006 |
| Alena Dostinova Ltd | Health & social care | 2026 |
| Alexandria Heights Ltd | Real estate | 2024 |
| Alfie Limited | Professional & scientific | 2002 |
| Alford Court Management Company Limited | Households | 1990 |
| ALH Project & Change Management Services Limited | Professional & scientific | 2024 |
| ALL About Bulk Limited | Transport & logistics | 2012 |
| ALL Gynaecology Ltd | Health & social care | 2024 |
| ALL Seasons GM Ltd | Administrative & support | 2003 |
| Allfast (UK) Limited | Wholesale & retail | 2000 |
| Allfast Tewkesbury Limited | Wholesale & retail | 2023 |
| Allright Enterprises Limited | Real estate | 2023 |
| Alno Product Services Limited | Manufacturing | 1992 |
| Alps Pizza Limited | Hospitality | 2024 |
| ALS Truckwash Ltd | Administrative & support | 2020 |
| Altai Capital Limited | Real estate | 2024 |
| ALTUS3 Limited | Administrative & support | 2017 |
| Aluplast (UK) Ltd | Wholesale & retail | 2026 |
| Ambient Agent Ltd | Information & communication | 2025 |
| Amelia’S Stores Ltd | Wholesale & retail | 2024 |