| Amiosec Ltd | Professional & scientific | 2014 |
| Amiosec Trustee Ltd | Extraterritorial | 2015 |
| AMY & Kelly Limited | Other services | 2010 |
| Andaman Energy & Infrastructure Limited | Financial services | 2017 |
| Anderson Information Solutions Limited | Information & communication | 1998 |
| Andrew Yates Software Ltd | Information & communication | 2014 |
| Angelvale Management Company Limited | Real estate | 2003 |
| ANP Stores Limited | Wholesale & retail | 2005 |
| ANT Hospitality Group Limited | Hospitality | 2025 |
| Anya Wood Public Relations Limited | Professional & scientific | 2018 |
| AP Academies Limited | Education | 2024 |
| AP Agri Enterprises Limited | Arts & recreation | 2014 |
| APG Developments Ltd | Construction | 2018 |
| Aporian Ltd | Information & communication | 2022 |
| App (UK) Limited | Wholesale & retail | 2003 |
| App Swansea Ltd | Real estate | 2025 |
| Aqua Canine Hydrotherapy Ltd | Other services | 2024 |
| Aqua-Dock Limited | Manufacturing | 2008 |
| Aquae Sulis Medical Ltd | Health & social care | 2022 |
| Aquasave Technologies Limited | Professional & scientific | 2000 |
| Architecture and Design Strategy Limited | Professional & scientific | 1999 |
| Arcvision Cyber Ltd | Information & communication | 2025 |
| Arden Consulting Ltd. | Real estate | 1998 |
| Arden Properties (Gloucestershire) Ltd | Real estate | 2013 |
| Arlw Property Ltd | Real estate | 2025 |
| Armour Communications Limited | Information & communication | 2014 |
| Armstrong Procter Group Ltd | Financial services | 2025 |
| Armstrong Procter Management Ltd | Real estate | 2025 |
| Armstrong Procter Properties Limited | Real estate | 2024 |
| Artyee Ltd | Wholesale & retail | 2022 |
| Arubus (Cheltenham) Limited | Professional & scientific | 2018 |
| Arubus Limited | Other services | 2018 |
| AS Sheep Dipping Ltd | Agriculture & fishing | 2019 |
| Ashchurch Flats Management Company Limited | Households | 2016 |
| Ashchurch Playgroup Tewkesbury Limited | Education | 2015 |
| Ashfield Power Limited | Energy | 2011 |
| ASK Indy Limited | Real estate | 2025 |
| Aspect Finance Ltd | Real estate | 2004 |
| Aspen Ground Care Commercial Ltd | Administrative & support | 2024 |
| Aspen Ground Care Ltd | Administrative & support | 2019 |
| Aston Catering Limited | Hospitality | 2022 |
| Aston Technologies Ltd | Wholesale & retail | 2021 |
| Aston Willows Ltd | Wholesale & retail | 2021 |
| Astra Lumos Limited | Construction | 2014 |
| Athw Holdings Limited | Financial services | 2025 |
| Atlantic Coast Studios Ltd | Manufacturing | 2003 |
| Atlas Sportstech Ltd | Extraterritorial | 2020 |
| Aubertin Financial Ltd | Financial services | 2025 |
| Aubury Computer Systems Limited | Information & communication | 2003 |
| Augustine Medical Ltd | Health & social care | 2016 |
| Auralis Group Limited | Financial services | 2025 |
| Austin Properties Ltd | Real estate | 2018 |
| Auto Ice Blast Ltd | Wholesale & retail | 2026 |
| Auto Precision Engineering Limited | Manufacturing | 2016 |
| Auto Trader Stock Supplies Limited | Administrative & support | 2023 |
| Autocolour Services Tewkesbury Limited | Wholesale & retail | 2012 |
| Automated Building & Energy Controls (Group) Ltd. | Construction | 2010 |
| Automated Building & Energy Controls (Holdings) Ltd. | Financial services | 2021 |
| Automated Building & Energy Controls Ltd. | Professional & scientific | 2004 |
| Autoparts & Diagnostics Limited | Wholesale & retail | 2003 |
| AV Assets Limited | Professional & scientific | 2007 |
| AV Rentals Ltd | Real estate | 2026 |
| Avanti_Transit LLP | | 2006 |
| Avon Court Management Co. (Tewkesbury) Ltd | Households | 2007 |
| Avon Investment Strategies Ltd | Financial services | 2025 |
| Avonside PHT Tewkesbury Ltd | Transport & logistics | 2023 |
| Awol Associates Limited | Other services | 2018 |
| AXD Special Projects Limited | Construction | 2019 |
| AXS Investments Ltd | Construction | 2021 |
| AXU Scaffolding Ltd | Construction | 2020 |
| Azams Properties Ltd | Real estate | 2018 |
| B J Belshaw Limited | Construction | 2000 |
| B. Sweet & Sons Limited | Other services | 2005 |
| Back Into Action Limited | Health & social care | 2004 |
| Bailey Automation Ltd | Professional & scientific | 2026 |
| Bailey Automotive Solutions Ltd | Wholesale & retail | 2014 |
| Bakewell Paige Ltd | Health & social care | 2019 |
| Barter Green Electrical Limited | Construction | 2020 |
| Bastian Property Limited | Real estate | 2025 |
| Bathrooms Gloucester Ltd | Wholesale & retail | 2018 |
| Batson Bidco Limited | Professional & scientific | 2016 |
| Battery Megastore Consultancy LLP | | 2011 |
| BB Fabrications UK Ltd | Manufacturing | 2008 |
| BBG Holdco Limited | Real estate | 2004 |
| BBG Lift Accommodation Services (NO 2) Limited | Real estate | 2013 |
| BBG Lift Accommodation Services Limited | Real estate | 2004 |
| BBG Lift Holdco (NO 2) Limited | Real estate | 2013 |
| BE Management Limited | Information & communication | 2003 |
| Beaver Estates Limited | Real estate | 1996 |
| Beckford Community Village Hall Limited | Other services | 2010 |
| Beckford Nature Reserve | Arts & recreation | 2007 |
| Beckford Silk Limited | Manufacturing | 1975 |
| Beete Builds Limited | Manufacturing | 2026 |
| Bennett's Advisory Services Limited | Professional & scientific | 2001 |
| Bennu Environmental Holdings Limited | Professional & scientific | 2026 |
| Bennu Environmental Limited | Professional & scientific | 2024 |
| Bespic Limited | Extraterritorial | 1974 |
| Beswick Partnership Limited | Professional & scientific | 2003 |
| Beyond Driving Limited | Education | 2022 |
| BHH Holdco Limited | Real estate | 2004 |