| THE Kathmandu Suit Company Ltd | Wholesale & retail | 2025 |
| THE Kenilworth Press Limited | Information & communication | 1989 |
| THE Kitchen Companions CIC | Hospitality | 2023 |
| THE Lazy PIG Kitchen Ltd | Hospitality | 2023 |
| THE Lindens Early Years CIC | Education | 2020 |
| THE Link Directories (Gloucester) Ltd | Administrative & support | 2009 |
| THE Made in Stroud Online Shop Ltd | Wholesale & retail | 2018 |
| THE Major Agency Ltd | Professional & scientific | 2008 |
| THE Medieval Hall Limited | Real estate | 2013 |
| THE Midlife Psychologist Ltd | Health & social care | 2026 |
| THE Movement Movement Ltd | Professional & scientific | 2018 |
| THE Natural Dispensary Limited | Wholesale & retail | 2007 |
| THE Nelson Trust | Health & social care | 1996 |
| THE Old Barn Ltd | Hospitality | 2023 |
| THE Old Malakoff Limited | Real estate | 2011 |
| THE One Shop Renewable Shop Limited | Construction | 2024 |
| THE Oscar Collective Ltd | Wholesale & retail | 2016 |
| THE Painswick Pharmacy Limited | Wholesale & retail | 1948 |
| THE Painswick Pooch LLP | | 2018 |
| THE Pirate Design Co. Limited | Information & communication | 2009 |
| THE Place Outside CIC | Administrative & support | 2018 |
| THE Play Tree Ltd | Health & social care | 2026 |
| THE Player Project Ltd | Education | 2026 |
| THE Queer Campfire Ltd | Wholesale & retail | 2022 |
| THE Radical Youth Space for Educations CIC | Education | 2025 |
| THE RAM Inn (Bussage) Limited | Wholesale & retail | 2011 |
| THE RAM Inn, South Woodchester Limited | Hospitality | 2024 |
| THE Recovery Club Limited | Health & social care | 2024 |
| THE Retreat Genie Ltd | Professional & scientific | 2020 |
| THE Rowans Physiotherapy Practice LLP | | 2016 |
| THE School of Health Limited | Extraterritorial | 2017 |
| THE School of Homeopathy Limited | Extraterritorial | 2017 |
| THE Scicomms Agency Limited | Professional & scientific | 2024 |
| THE Service Office of Families Anonymous (UK, Eire and Gibraltar) Limited | Health & social care | 2012 |
| THE Ship Inn(Stroud) Ltd. | Hospitality | 2015 |
| THE Sky Mining Company Limited | Manufacturing | 2016 |
| THE Sleep Well Project Limited | Extraterritorial | 2018 |
| THE Sorensen Group Limited | Professional & scientific | 2025 |
| THE Spinney (Plymouth) NO.5 Management Company Limited | Households | 1995 |
| THE Stencil Studio Limited | Wholesale & retail | 2012 |
| THE Steppes Care Limited | Health & social care | 2001 |
| THE Street Consulting Limited | Professional & scientific | 2013 |
| THE Stroud Brewery Limited | Manufacturing | 2004 |
| THE Teasel Yurts Limited | Hospitality | 2018 |
| THE Textile Gallery Community Interest Company | Arts & recreation | 2025 |
| THE Thing IS Limited | Information & communication | 2022 |
| THE Thrupp Management Company Limited | Financial services | 2001 |
| THE Tree People Gloucestershire Ltd | Agriculture & fishing | 2026 |
| THE Trenchard Practice Limited | Health & social care | 2021 |
| THE Village Inn Brew House Ltd | Hospitality | 2016 |
| THE Vine Shop Ltd. | Wholesale & retail | 2020 |
| THE Wedding Barn Limited | Wholesale & retail | 2019 |
| THE Weighbridge Inn Limited | Hospitality | 2023 |
| THE White Practice Limited | Health & social care | 2019 |
| THE Wilder Network C.I.C. | Professional & scientific | 2022 |
| THE Wire-Watcher Company Limited | Professional & scientific | 1993 |
| THE Wool Loft MGT Co Ltd | Households | 2005 |
| THE Woolpack Inn Limited | Hospitality | 1999 |
| THE Workers Policy Project CIC | Other services | 2024 |
| THE Yard Cafe & Kitchen Ltd | Hospitality | 2022 |
| Thecommscollective.Club Limited | Professional & scientific | 2013 |
| Theia&Co. Ltd | Health & social care | 2015 |
| Theo Deproost Photography Limited | Professional & scientific | 2019 |
| Thesensationalsandwichshop Ltd | Wholesale & retail | 2025 |
| Thinkforward Engineering Ltd | Professional & scientific | 2024 |
| Thinking Human Ltd | Professional & scientific | 2024 |
| Thirst Link (AS) Limited | Professional & scientific | 2013 |
| This Ends NOW Ltd | Other services | 2022 |
| This House Limited | Real estate | 2020 |
| Thomas Bennett Estates Ltd | Real estate | 2023 |
| Thomas Keble School | Education | 2011 |
| Thomson Design & Build Ltd | Construction | 2018 |
| Thorned Ltd | Professional & scientific | 2017 |
| Thrive Developments Limited | Real estate | 1999 |
| Thumbstone Consultancy Services Limited | Professional & scientific | 2023 |
| Tidal Forces Ltd | Energy | 2025 |
| Tidal Tales Collective CIC | Arts & recreation | 2021 |
| TIM Telling Ltd | Information & communication | 2014 |
| TIM Wilkins Training Ltd | Professional & scientific | 2022 |
| Timberwoolf Ltd | Manufacturing | 2018 |
| Time Banks UK | Education | 2002 |
| Time Control Technical Works UK Ltd | Construction | 2022 |
| TIME2STUDY Publications Limited | Information & communication | 1990 |
| Tinglestone Holdings Ltd | Financial services | 2024 |
| Tixiom Limited | Information & communication | 1995 |
| TJ Wilkins Limited | Other services | 2003 |
| TKH France Lynch Ltd | Hospitality | 2022 |
| TKJ Ceramics Limited | Arts & recreation | 2022 |
| TO BE Well Limited | Health & social care | 2017 |
| Tobacconist Farm Limited | Agriculture & fishing | 2009 |
| TOD Conference Production Ltd | Administrative & support | 2025 |
| Togtech Limited | Wholesale & retail | 2021 |
| TOM Dick & Debbie Productions Ltd | Information & communication | 1999 |
| Tomkins & White Services Ltd | Manufacturing | 2001 |
| Tony F Falcone Ltd | Construction | 2018 |
| Top Cable Accessories Limited | Wholesale & retail | 2000 |
| Topcon Technology Limited | Manufacturing | 2012 |
| Topps Marketing Ltd | Professional & scientific | 2024 |
| Torchtech Ltd | Information & communication | 2016 |
| Torino Nice Rally Ltd | Arts & recreation | 2018 |