| Systemic Metavision Ltd | Extraterritorial | 2024 |
| T C Grayling Ltd | Information & communication | 2017 |
| T F Commercial Finance Ltd | Financial services | 2025 |
| T Hawkins Limited | Transport & logistics | 2008 |
| T.R. King Limited | Manufacturing | 1994 |
| TAG Properties (Stroud) Limited | Real estate | 2025 |
| Tailored Collective Ltd | Construction | 2025 |
| Talaus Limited | Manufacturing | 2013 |
| Tanager Consultants Limited | Professional & scientific | 2025 |
| Tangerine Search and Selection Limited | Administrative & support | 2011 |
| Taotao Limited | Wholesale & retail | 2017 |
| TAP & Tales Ltd | Hospitality | 2025 |
| Task Force Electrical Solutions Ltd | Construction | 2026 |
| Tauneutrino Ltd | Information & communication | 2026 |
| Tavender Property Limited | Real estate | 2005 |
| Tavener Design Ltd | Professional & scientific | 2022 |
| Taylor Financial Recruitment Ltd | Professional & scientific | 2017 |
| Taylor Rankin Construction Ltd | Construction | 2021 |
| Tcsf Holdings Ltd | Financial services | 2024 |
| Teaching Public Service in the Digital AGE Community Interest Company | Education | 2022 |
| Team Barwell Ltd | Professional & scientific | 2018 |
| TEC Gardens & Grounds Ltd | Administrative & support | 2024 |
| Tech-G Ltd | Information & communication | 2014 |
| Tectona Partnership Limited | Professional & scientific | 2012 |
| TED & Sons Roofing Ltd | Construction | 2022 |
| TED James Design Limited | Manufacturing | 2010 |
| Telemental Limited | Professional & scientific | 2016 |
| Tempest Healthcare Consulting Ltd | Professional & scientific | 2018 |
| Tenens PTC Limited | Administrative & support | 2017 |
| Tenens Sharpness LLP | | 2012 |
| Terrascapes Limited | Administrative & support | 2003 |
| Terry Doran Limited | Construction | 2003 |
| Tessad Limited | Financial services | 2022 |
| Tetbury Nursery Playgroup Limited | Education | 2004 |
| TF Digital Ltd | Information & communication | 2020 |
| Tgdh Ltd | Health & social care | 2009 |
| That Cotswold Claire Home & Interiors Ltd | Professional & scientific | 2025 |
| That's a Good Question Ltd | Professional & scientific | 2020 |
| THE Adventure Team Limited | Arts & recreation | 2015 |
| THE ALL Saints Fields Management Company Limited | Households | 2004 |
| THE Amberley Inn Ltd | Hospitality | 2025 |
| THE Antique MAP Shop Limited | Wholesale & retail | 2004 |
| THE ARK Cycles Ltd | Wholesale & retail | 2018 |
| THE Artful Green Limited | Arts & recreation | 2024 |
| THE Artisan Baker Montpellier Ltd | Wholesale & retail | 2022 |
| THE Artisan Baker Partnership Limited | Manufacturing | 2020 |
| THE Beauty Barn Stroud Limited | Other services | 2019 |
| THE BEE Observatory C.I.C. | Agriculture & fishing | 2020 |
| THE Bespoke Flower Co. Ltd | Wholesale & retail | 2018 |
| THE Big Swing Company Limited | Professional & scientific | 2006 |
| THE Birches (Uplands) Limited | Households | 1994 |
| THE Biryani POT Ltd | Hospitality | 2024 |
| THE Boho Bakery Limited | Manufacturing | 2020 |
| THE Bollington PUB Co Ltd | Hospitality | 2020 |
| THE Bowbridge Arms Ltd | Hospitality | 2024 |
| THE Brainy-Ac Ltd | Education | 2023 |
| THE Carpenters Arms Westrip Limited | Hospitality | 2019 |
| THE Change Engine Limited | Professional & scientific | 2007 |
| THE Clay Loft Limited | Education | 2016 |
| THE Coaching Pharmacist Ltd | Health & social care | 2025 |
| THE Colony Solution Ltd | Information & communication | 2021 |
| THE Commercial Group Ltd | Administrative & support | 2020 |
| THE Composite Scaffolding Company Limited | Manufacturing | 2015 |
| THE Convent in the Hills Limited | Real estate | 2014 |
| THE Corner House (Stroud) Limited | Hospitality | 2018 |
| THE Cossack Square (Nailsworth) Flat Management Company Limited | Administrative & support | 1988 |
| THE Cotswold Bowls Club Limited | Arts & recreation | 2017 |
| THE Cotswold Canals Trust | Other services | 1975 |
| THE Cotswold Production Company Limited | Arts & recreation | 2018 |
| THE Cotswold Solar Company Ltd | Construction | 2023 |
| THE Courtyard Commercial Company Limited | Households | 2012 |
| THE Courtyard Management Company (Stonehouse) Limited | Real estate | 2006 |
| THE Craftology Project CIC | Arts & recreation | 2022 |
| THE Daily Bark Ltd | Other services | 2026 |
| THE Doggy Playschool Company Limited | Other services | 2020 |
| THE Door Youth Project | Health & social care | 2009 |
| THE Edna Consultancy Ltd | Professional & scientific | 2024 |
| THE Familyship Practice Ltd | Health & social care | 2017 |
| THE Feathers Hotel Limited | Hospitality | 2023 |
| THE Finance Department (Stroud) Ltd | Professional & scientific | 1996 |
| THE Firs Stroud Limited | Real estate | 2018 |
| THE Fitness Mill (Stroud) Ltd | Arts & recreation | 2009 |
| THE FIX IT Group Limited | Extraterritorial | 2025 |
| THE Food Partnership Limited | Professional & scientific | 2010 |
| THE Foundry Community Ltd | Other services | 2025 |
| THE Fresh Standard Brew Co Ltd | Manufacturing | 2020 |
| THE Garden Cook Ltd | Wholesale & retail | 2021 |
| THE Gorge Inn Newmarket Ltd | Hospitality | 2025 |
| THE Grass Guys Limited | Administrative & support | 2025 |
| THE Great Plate CIC | Hospitality | 2023 |
| THE Green Britain Academy Limited | Education | 2021 |
| THE Grove Stroud CIC | Education | 2023 |
| THE Health Plantry Limited | Manufacturing | 2024 |
| THE Heat Exchange Sauna Company Ltd | Arts & recreation | 2026 |
| THE Heights (Vatch View) Management Company Limited | Households | 2002 |
| THE Hithe Residents Limited | Other services | 2007 |
| THE Holy Water Group Limited | Hospitality | 2025 |
| THE Horns Road PUB Ltd | Hospitality | 2024 |
| THE Introspective Tiger Ltd | Wholesale & retail | 2025 |
| THE Kairos Project CIC | Professional & scientific | 2014 |