| Sleepy Frog Ltd | Information & communication | 2025 |
| SM Wheeler Investments Ltd | Construction | 2026 |
| Small Wind Generation Limited | Energy | 2011 |
| Smart Tech NOW Limited | Wholesale & retail | 2019 |
| Smug Oak Limited | Real estate | 2020 |
| Snow Business Holdings Ltd | Professional & scientific | 2018 |
| Snow Business International Ltd | Information & communication | 1996 |
| Snow Business Limited | Information & communication | 2016 |
| Soap Folk Limited | Manufacturing | 2021 |
| Social Value Bids Limited | Professional & scientific | 2024 |
| Sofia's Chinese Kitchen Limited | Hospitality | 2024 |
| Softlogic Property LLP | | 2006 |
| Softlogic Solutions Limited | Information & communication | 1990 |
| Soiltonics Limited | Manufacturing | 2023 |
| Solar Synergy Solutions Ltd | Construction | 2025 |
| Soley Performance Limited | Arts & recreation | 2014 |
| Solid State Structures Ltd | Professional & scientific | 2019 |
| Soloclamp Ltd | Manufacturing | 2022 |
| Soloheat Limited | Administrative & support | 2002 |
| Solution Business Consulting Ltd | Wholesale & retail | 2013 |
| Solutions for Evolution Limited | Professional & scientific | 1999 |
| Somerton Wind Park Limited | Energy | 1998 |
| Sonewoven Design Ltd | Professional & scientific | 2025 |
| Song Theatre Ltd | Arts & recreation | 2012 |
| Sophie Hooper Ltd | Wholesale & retail | 2024 |
| Sorrento Italian Restaurants Limited | Hospitality | 2008 |
| Soumik Datta Arts Limited | Arts & recreation | 2012 |
| Sound Seeds Ltd | Administrative & support | 2016 |
| Sourcy Ltd | Arts & recreation | 2018 |
| South Avenue Fleet Sales Limited | Wholesale & retail | 2003 |
| South Bristol Grace Network CIC | Professional & scientific | 2024 |
| South West Buildings Ltd | Administrative & support | 2019 |
| Southfields Consulting Limited | Professional & scientific | 2017 |
| SP Renewables Limited | Construction | 2023 |
| Spacehoppers Co-Working Limited | Real estate | 2015 |
| Spaceship Studios Ltd | Professional & scientific | 2016 |
| Specialist Building and Renovation Ltd | Construction | 2026 |
| Spectra Create Limited | Manufacturing | 2016 |
| Spectrum Clinic Limited | Health & social care | 2025 |
| Spectrum Support Limited | Professional & scientific | 2015 |
| Spectrum Warriors Ltd | Wholesale & retail | 2025 |
| Spencer Discounts Limited | Administrative & support | 2022 |
| Spherical Wheel Limited | Extraterritorial | 2012 |
| Spifey Ltd | Information & communication | 2019 |
| Spon.Io Ltd | Information & communication | 2019 |
| Sporting Cohesion Ltd | Arts & recreation | 2023 |
| Springfield Investments Limited | Construction | 1958 |
| Spruce Sauna Ltd | Other services | 2025 |
| SSD (2014) Limited | Manufacturing | 1988 |
| Ssocks Ltd | Manufacturing | 2025 |
| SST Consulting Limited | Professional & scientific | 2026 |
| ST Erth Consultants LLP | | 2015 |
| St.Clair Care Limited | Health & social care | 2003 |
| Stable Yard Management Company Limited | Households | 1984 |
| Stackboard Ltd | Information & communication | 2008 |
| Stag Developments Limited | Real estate | 1985 |
| Stag Homes (Gloucestershire) Limited | Construction | 2016 |
| Stag Homes (Severnside) Ltd | Construction | 2017 |
| Stancombe Stone Limited | Manufacturing | 1994 |
| Stand + Stare Ltd | Arts & recreation | 2011 |
| Star - Speech Therapy Angela Reimer Ltd | Health & social care | 2025 |
| Starkey Hire Limited | Wholesale & retail | 1991 |
| Starkworks Limited | Other services | 2011 |
| Starpad Properties Ltd | Real estate | 2017 |
| Starside Media Limited | Professional & scientific | 2017 |
| Statehouse Capital Management Limited | Real estate | 2017 |
| Statehouse Consultancy Limited | Professional & scientific | 2004 |
| Statham Custom Cabins Ltd | Construction | 2025 |
| STC Services (Nailsworth) Ltd | Wholesale & retail | 2023 |
| Steed and ME Ltd | Wholesale & retail | 2020 |
| Step Outside Limited | Education | 2021 |
| Stephen Lenthall Photography Limited | Arts & recreation | 2007 |
| Sterimatic Worldwide Limited | Manufacturing | 2002 |
| Sterling Offices & Storage Ltd | Financial services | 2019 |
| Sterling Storage Solutions Limited | Transport & logistics | 2015 |
| Steve Russell Studios Limited | Professional & scientific | 2003 |
| Steven J Hawkins Plumbing & Heating Ltd | Construction | 2022 |
| Stewart Estate Training Ltd | Arts & recreation | 2013 |
| Stimson-Machers Limited | Education | 2016 |
| Stinchcombe Hill Trust | Arts & recreation | 2011 |
| Stirling Management Limited | Construction | 2003 |
| Stirzaker Psychology Limited | Health & social care | 2019 |
| Stoa Carpentry & Construction Ltd | Manufacturing | 2024 |
| Stockdale Trading Company Limited | Real estate | 2014 |
| Stone Art Cotswold Limited | Construction | 2013 |
| Stone Court Residents Association Limited | Households | 2001 |
| Stone Raft Creative Limited | Information & communication | 2008 |
| Stonecomms Limited | Professional & scientific | 2023 |
| Stonehouse Exhaust & Tyre Centre Limited | Wholesale & retail | 2013 |
| Stonewalling and Fencing Limited | Construction | 2004 |
| Stony Broke Limited | Real estate | 2014 |
| Stop Ecocide International Ltd | Other services | 2017 |
| Storm Skills Training CIC | Health & social care | 2011 |
| Story Creative Limited | Manufacturing | 2010 |
| Stoubaek Limited | Professional & scientific | 2003 |
| Strachan and Company Limited | Extraterritorial | 1990 |
| Strategic Intelligence and Associates Limited | Professional & scientific | 2009 |
| Stratford Jewellery and Loan Limited | Wholesale & retail | 2013 |
| Strawberry Property Investment Ltd | Real estate | 2020 |
| Street Potential Academy Community Interest Company | Health & social care | 2019 |