| Sama Creatives Ltd | Professional & scientific | 2023 |
| Samet Limited | Information & communication | 2004 |
| Sammol Engineering Ltd | Professional & scientific | 1996 |
| Sandy Wind Turbine Limited | Professional & scientific | 2007 |
| Sarah FOX Limited | Professional & scientific | 2014 |
| Sarah Hardaker Limited | Professional & scientific | 2003 |
| Sarah Slater Consulting Limited | Professional & scientific | 2016 |
| Sath Foods Ltd | Hospitality | 2022 |
| Sath Properties Limited | Real estate | 2020 |
| Satsu Limited | Real estate | 2017 |
| Savy Systems Limited | Professional & scientific | 2016 |
| Saymor Furnishers Limited | Wholesale & retail | 2003 |
| SB Energy Group Ltd | Construction | 2025 |
| SBC Properties Limited | Mining & quarrying | 1985 |
| Sbjr Consultancy Services Limited | Information & communication | 2016 |
| Sbpx Media Limited | Information & communication | 2000 |
| SC Customer Services Ltd | Health & social care | 2002 |
| SC Sports Bars Limited | Hospitality | 2024 |
| Scarlett Recruitment Limited | Administrative & support | 2020 |
| Schoonersail Limited | Other services | 2007 |
| Scott Hillam Associates LLP | | 2016 |
| Scott Murray Design Ltd | Manufacturing | 2024 |
| Scumble Goosie LLP | | 2008 |
| Scwair One Ltd | Administrative & support | 2019 |
| SDL Civils Limited | Construction | 2020 |
| SE Commercial Limited | Real estate | 2016 |
| Seabrook Sporting Limited | Arts & recreation | 2022 |
| Secured Property Holdings Ltd | Financial services | 2017 |
| Secured Property Management Limited | Real estate | 2017 |
| Seeding Transformation Ltd | Professional & scientific | 2025 |
| Select Property Developments (Gloucester) Ltd | Administrative & support | 1988 |
| Sellick Property Limited | Real estate | 2022 |
| Selsley Foods Limited | Wholesale & retail | 2011 |
| Semdar Properties Ltd | Construction | 2025 |
| Semdar Services Ltd | Construction | 2025 |
| Sensationalsandwiches Ltd | Wholesale & retail | 2024 |
| Sensitive Soul Club Ltd | Wholesale & retail | 2025 |
| Sephira Ltd. | Information & communication | 1996 |
| Seraville Limited | Hospitality | 1997 |
| Serene Communications Limited | Administrative & support | 2003 |
| Sero SED Serio Ltd | Information & communication | 2024 |
| Service 1ST Ltd | Households | 2023 |
| Service BR Limited | Wholesale & retail | 2025 |
| Severn Damp Proofing Ltd | Construction | 2017 |
| Severn Poultry Limited | Manufacturing | 1995 |
| Severn Vale Truck Movements Limited | Transport & logistics | 2011 |
| SF Events Ltd | Administrative & support | 2013 |
| SF Medical Ltd | Health & social care | 2021 |
| SG Landscaping Ltd | Construction | 2022 |
| Sgim Stroud Limited | Wholesale & retail | 2023 |
| SGS College Limited | Extraterritorial | 2024 |
| Shampoodles Dog Salon Ltd | Other services | 2022 |
| Shannon Craig Wealth Management Ltd | Financial services | 2024 |
| Shaplacurry Limited | Hospitality | 2024 |
| Sheep and Stone Ltd | Arts & recreation | 2024 |
| Sherman Street Ltd | Professional & scientific | 2016 |
| Shibumi Style Ltd | Wholesale & retail | 2014 |
| Shift to Calm CIC | Other services | 2025 |
| Shine Nails & Beauty Limited | Other services | 2010 |
| Shiner Chappell Property Ltd | Agriculture & fishing | 2018 |
| Shiner Mitchell Fisher & Co Limited | Professional & scientific | 1998 |
| Shining Moon Property Ltd | Real estate | 2019 |
| Shiny Goodness Limited | Wholesale & retail | 2000 |
| Shipped OFF and Wrecked UP Ltd | Hospitality | 2026 |
| Shiraz Akoo Ltd | Health & social care | 2013 |
| Shire Training Workshops Limited | Hospitality | 1979 |
| Shoebox Capital Ltd | Real estate | 2018 |
| Shoebox Consulting Ltd | Professional & scientific | 2023 |
| Shoebox Group Holdings Ltd | Financial services | 2023 |
| Shona Ward T/A Learning Curve Limited | Professional & scientific | 2024 |
| Shoot Unit Limited | Professional & scientific | 2011 |
| Shooters Bottom Wind Park Ltd | Energy | 2000 |
| Showponies Ltd | Arts & recreation | 2020 |
| Shunen Ltd | Wholesale & retail | 2016 |
| Shutter Artz Photography Ltd | Professional & scientific | 2025 |
| Sidestep 4 Limited | Information & communication | 2006 |
| Silvadis Estates Limited | Administrative & support | 2011 |
| Silver Street Consultants Ltd | Professional & scientific | 2010 |
| Silverbeaver Ltd | Manufacturing | 2026 |
| Silverwood Land & Developments Ltd | Real estate | 2025 |
| Silverwood Property Management Services Limited | Households | 2018 |
| Silvey Properties Ltd | Real estate | 2023 |
| Simply Health Cotswolds Ltd | Other services | 2025 |
| Siquidem Ltd | Real estate | 2024 |
| Sirbris Ltd | Manufacturing | 2023 |
| Sisterhood of Women Consulting Ltd | Professional & scientific | 2025 |
| Sita Retreats Limited | Administrative & support | 2026 |
| Skaiteshill Kennels (Cotswolds) Ltd | Other services | 2023 |
| Skibidi Sigma Limited | Information & communication | 2024 |
| Skillscanr Ltd | Information & communication | 2025 |
| Skjold Protection Ltd | Administrative & support | 2025 |
| Sky Fabrications Ltd | Manufacturing | 2017 |
| Skyhook Supply Company Limited | Professional & scientific | 2014 |
| Skyloft Collective Ltd | Real estate | 2024 |
| Skyrocket Creative Limited | Professional & scientific | 2012 |
| Skyscanworld Ltd | Information & communication | 2020 |
| Slab Stroud Limited | Construction | 2022 |
| Slad Brook Limited | Agriculture & fishing | 2000 |
| Slad Valley Songs Limited | Information & communication | 2022 |
| Sladebank Woods C.I.C. | Agriculture & fishing | 2016 |