| Okada Ltd | Wholesale & retail | 2025 |
| Old Bakery (Minchinhampton) Management Company Limited | Real estate | 2020 |
| Old Market Takeaway Limited | Hospitality | 2018 |
| Old School Close (Nailsworth) Management Company Limited | Administrative & support | 2000 |
| Old School Learning Limited | Education | 2014 |
| Older Investment Management Ltd | Extraterritorial | 2014 |
| Olive Medical Limited | Information & communication | 2010 |
| Olshwrld Limited | Financial services | 2024 |
| Olympic Cars (Stroud) Limited | Wholesale & retail | 1992 |
| Omniball Ltd | Professional & scientific | 2009 |
| Omnicaster Limited | Manufacturing | 2012 |
| Omnitrack Holdings Ltd | Real estate | 2025 |
| Omnitrack Italia Ltd | Extraterritorial | 2013 |
| Omnitrack Limited | Extraterritorial | 2009 |
| Omnitrack North America Limited | Manufacturing | 2009 |
| Once in a Blue Moon Limited | Hospitality | 2015 |
| Onetap Applications Ltd | Information & communication | 2018 |
| Onpoint Windows & Doors Ltd | Construction | 2024 |
| Oolite Limited | Information & communication | 2019 |
| Oossee Limited | Wholesale & retail | 2025 |
| Openhire Limited | Information & communication | 2023 |
| Optimal Outcomes Ltd | Financial services | 2015 |
| Optimised Natural Health Ltd | Health & social care | 2020 |
| Optimum Energy Limited | Professional & scientific | 1998 |
| Optimus Property Ltd | Real estate | 2012 |
| Opus Stroud Limited | Construction | 2022 |
| Orange Land and Property Ltd | Construction | 2018 |
| Oranges for Justice CIC | Wholesale & retail | 2024 |
| Orbicular Consulting Limited | Professional & scientific | 2023 |
| Orcavia Limited | Professional & scientific | 2025 |
| Orchard Financial Associates LLP | | 2020 |
| Orchard Haven Management Limited | Real estate | 2007 |
| Orchid Collective CIC | Education | 2025 |
| Oriental Wholesale Stroud LLP | | 2025 |
| Original House Limited | Wholesale & retail | 2008 |
| Original Sash Window Solutions Limited | Construction | 2011 |
| Oski Limited | Administrative & support | 2026 |
| OUR Nooks Ltd | Real estate | 2023 |
| Outlaws Barbershop Ltd | Other services | 2026 |
| Outpost Gamma Alpha Limited | Real estate | 2023 |
| Outsideliving Ltd. | Wholesale & retail | 2009 |
| Outsource Safety Limited | Administrative & support | 1998 |
| Owain Atkins Cabinet Makers Limited | Manufacturing | 2003 |
| Oxfordshire Smile Ltd | Health & social care | 2021 |
| Ozzy Tate Ltd | Wholesale & retail | 2022 |
| P C Associates Limited | Manufacturing | 1989 |
| P J Dennis Limited | Professional & scientific | 2013 |
| P.J Marks Limited | Financial services | 2024 |
| P.K. Wills Services Ltd. | Transport & logistics | 1997 |
| P.L.Allard Building & Roofing Ltd | Construction | 2017 |
| P4 Racing Ltd | Administrative & support | 2019 |
| Pact Property and Assets Ltd | Administrative & support | 2018 |
| Padin Electrical Services Limited | Construction | 2003 |
| Padin Homes Ltd | Real estate | 2017 |
| Paganhill Community Group CIC | Other services | 2019 |
| Painswick Collective CIC | Information & communication | 2023 |
| Painswick Engineering Ltd | Manufacturing | 2020 |
| Painswick Garden Estate Limited | Wholesale & retail | 1987 |
| Painswick Heights Management Company Limited | Households | 2001 |
| Painswick Property Limited | Real estate | 2017 |
| Painswick Property Management Limited | Agriculture & fishing | 2023 |
| Painswick RFC Trading Limited | Arts & recreation | 2016 |
| Painswick Rugby Football Club | Arts & recreation | 2012 |
| Paint a POT Ltd | Arts & recreation | 2014 |
| Paint IT 2018 Ltd | Construction | 2018 |
| Paintball Raiders Ltd | Arts & recreation | 2021 |
| Panelitix.Ai Ltd | Information & communication | 2023 |
| Paperpost Limited | Information & communication | 2009 |
| Paramount Homes (Cheltenham) Limited | Construction | 2006 |
| Parcot Limited | Professional & scientific | 2021 |
| Park Villa (Stroud) Management Limited | Households | 2005 |
| Parkes Painswick Properties Ltd | Real estate | 2025 |
| Parktrek Limited | Construction | 1986 |
| Parlour Farm Industrial Leasing Limited | Real estate | 2021 |
| Partnered Learning Limited | Wholesale & retail | 2016 |
| Partnering Power Ltd | Professional & scientific | 2015 |
| Partridge Homes (Cotswolds) Limited | Construction | 2005 |
| Pates Estates Ltd | Real estate | 2025 |
| Pathway Pioneer Housing CIC | Real estate | 2025 |
| Pathways to Ventures C.I.C. | Education | 2015 |
| Patrick Doherty Design Limited | Arts & recreation | 2003 |
| Paul & Co (UK) Ltd | Extraterritorial | 2008 |
| Paul a Morris General Building Limited | Construction | 2006 |
| Paul Berry Technical Ltd | Professional & scientific | 2005 |
| Paul Campbell (Glos) Limited | Other services | 2015 |
| Paul Maze Roofing Ltd | Construction | 2016 |
| Pauls Construction Innovations Ltd | Construction | 2026 |
| Pawfection Hospitality Limited | Hospitality | 2024 |
| Pawsitive Dog Company Ltd | Other services | 2024 |
| Payne and Watts Limited | Hospitality | 2011 |
| PAYROLL4ACCOUNTANTS Ltd | Other services | 2019 |
| PD & JE Associates Limited | Wholesale & retail | 2012 |
| Pdifs LLP | | 2017 |
| Peak Rider Ltd | Education | 2024 |
| Peanuts Deli Ltd | Hospitality | 2024 |
| Penna Homecare Limited | Health & social care | 2013 |
| Perception Photography Ltd | Professional & scientific | 2007 |
| Performance Legacy Limited | Professional & scientific | 2015 |
| Peritect Ltd. | Professional & scientific | 2014 |
| Perpetual Stew Ltd | Arts & recreation | 2025 |