| Nails 3031 Ltd | Other services | 2026 |
| Nailsworth Accountancy Limited | Professional & scientific | 2013 |
| Nailsworth Eyecare Ltd | Wholesale & retail | 2019 |
| Nailsworth Limited | Professional & scientific | 2010 |
| Nailsworth MOT Centre Limited | Wholesale & retail | 2016 |
| Nailsworth Natural Health Clinic Ltd | Health & social care | 2014 |
| Nailsworth Tyre Centre Limited | Wholesale & retail | 2003 |
| Nailsworth Wines and Spirits Limited | Wholesale & retail | 2022 |
| Natural Cookery School Limited | Education | 2023 |
| Naturally Bycipi Ltd | Manufacturing | 2021 |
| Naughton Consulting Limited | Professional & scientific | 1999 |
| Navitex Ltd | Administrative & support | 2026 |
| NCE Projects and Services Ltd | Professional & scientific | 2024 |
| Ncom Services Limited | Professional & scientific | 2002 |
| Nearbuywines.Com Limited | Wholesale & retail | 2021 |
| Neatly Ltd | Wholesale & retail | 2019 |
| Nebraska Trading Limited | Professional & scientific | 1993 |
| NEOS48 Ltd | Hospitality | 2023 |
| Nephilim Construction Limited | Construction | 2022 |
| Nephilim Group Limited | Construction | 2022 |
| Neuroverse Community C.I.C. | Education | 2025 |
| New Beginnings Hadlow Down Limited | Agriculture & fishing | 2018 |
| New Current Ltd | Construction | 2020 |
| New Power Company Limited | Financial services | 2001 |
| New ST House (Painswick) Property Management Limited | Real estate | 2017 |
| Newman Contracting (Stroud) Limited | Construction | 2016 |
| Newman Heating (Stroud) Limited | Construction | 2018 |
| Newplex Limited | Hospitality | 2018 |
| Next Generation Wind Holdings Limited | Financial services | 2003 |
| Next Level Installations Ltd | Construction | 2021 |
| Nextgen NOW Ltd | Information & communication | 2022 |
| Nice Work (UK) Limited | Hospitality | 2003 |
| Niche Ecology Ltd | Professional & scientific | 2024 |
| Nicholas Curram Limited | Professional & scientific | 2000 |
| Nick Haile Holdings Limited | Financial services | 2022 |
| Nick Miles Building Contractors Limited | Construction | 2003 |
| Nicky Doodson Flowers Limited | Wholesale & retail | 2009 |
| Nicky's Driving School Limited | Education | 2025 |
| Nicole Kelly Limited | Professional & scientific | 2025 |
| Niel Kempson Associates Limited | Professional & scientific | 2018 |
| Nigel Scotford Limited | Wholesale & retail | 1998 |
| Nightingale Property Group Ltd | Real estate | 2023 |
| Nikki Assists Limited | Administrative & support | 2014 |
| Nimbus Flies Limited | Education | 2021 |
| Nishana Enterprises Limited | Administrative & support | 2008 |
| Nisus Management Limited | Professional & scientific | 1997 |
| NJO Designs Limited | Professional & scientific | 2018 |
| NJX Ltd | Wholesale & retail | 2025 |
| Nkech Limited | Manufacturing | 2019 |
| NO 3 the Salon Ltd | Other services | 2019 |
| NO Nibble Ltd | Information & communication | 2023 |
| NO Plan Limited | Information & communication | 2014 |
| Noble Art Design Limited | Wholesale & retail | 2004 |
| Noble Metals Limited | Wholesale & retail | 2025 |
| Noble Vision Foundation CIC | Other services | 2025 |
| Noni's Coffee Roasters Ltd | Manufacturing | 2020 |
| Nook Custom Carpentry Limited | Construction | 2015 |
| North of NOW Consulting Ltd | Professional & scientific | 2024 |
| Northsound Limited | Manufacturing | 2010 |
| Nothing BUT Navy Ltd. | Wholesale & retail | 2012 |
| Notria Limited | Real estate | 2000 |
| Nottgrove Limited | Real estate | 2016 |
| Novalis Professional Services Limited | Construction | 2012 |
| Novalis Trust | Education | 2004 |
| Novel Engineering Consultants EOT Limited | Administrative & support | 2021 |
| Novel Engineering Consultants Ltd | Professional & scientific | 2007 |
| Npetc Limited | Real estate | 2021 |
| NR 23 Limited | Hospitality | 2024 |
| NRH Motors Limited | Wholesale & retail | 2012 |
| NS Surveying & Design Ltd | Professional & scientific | 2014 |
| NSC Music Ltd | Information & communication | 2021 |
| NSC Records Ltd | Information & communication | 2021 |
| Number 18 Durdham Park (Bristol) Limited | Households | 1965 |
| Nursery at Miserden Limited | Wholesale & retail | 2020 |
| NVJ Investments UN Limited | Wholesale & retail | 2025 |
| NWS CNC (Stroud) Limited | Manufacturing | 2016 |
| NÜ Publishing Ltd | Information & communication | 2026 |
| O&M Properties Limited | Real estate | 2017 |
| Oak & ASH & Thorn Ltd | Arts & recreation | 2024 |
| Oak Blue Consulting Ltd | Professional & scientific | 2026 |
| Oakbrook Orchard Community Interest Company | Agriculture & fishing | 2024 |
| Oakdale Wealth Limited | Financial services | 2021 |
| Oakleigh Joinery Ltd | Construction | 2002 |
| Oakley & Edwards Limited | Construction | 2002 |
| Oakridge Wealth Management Ltd | Financial services | 2015 |
| Oaksey & Kemble Commercials Limited | Administrative & support | 2005 |
| Oaksey Garage Limited | Wholesale & retail | 2005 |
| Oasis Events Limited | Hospitality | 2007 |
| Oblyx Developments Limited | Information & communication | 2008 |
| Obsidian Imaging Ltd | Extraterritorial | 2025 |
| Obtac Limited | Education | 2008 |
| Ochre Consultancy Limited | Information & communication | 2009 |
| Ocker Environmental Ltd | Professional & scientific | 2019 |
| Ocktra.Io Ltd | Information & communication | 2024 |
| OCM Events Limited | Administrative & support | 2017 |
| OFF Grid Productions Ltd | Information & communication | 2020 |
| OH Grape Ltd | Wholesale & retail | 2021 |
| Oilygem Ltd | Information & communication | 2020 |
| Oishii Haha Limited | Wholesale & retail | 2025 |
| OJ Williams Accounting Limited | Professional & scientific | 2013 |