| Ladyshape Limited | Arts & recreation | 2010 |
| LAH DE DAH Ltd | Education | 2019 |
| Lambsholme Ltd | Real estate | 2018 |
| Landford & SON Ltd | Hospitality | 2003 |
| Landscaping Gloucestershire Ltd | Administrative & support | 2026 |
| Larkhill Associates Ltd | Professional & scientific | 2024 |
| Laroc Company Ltd | Financial services | 2025 |
| Lauben Partners Ltd | Professional & scientific | 2024 |
| Laurence Neville Limited | Professional & scientific | 2007 |
| Lavendermassage Limited | Other services | 2025 |
| Lawn Developments Limited | Real estate | 1984 |
| Lawrie Aesthetics Ltd | Other services | 2025 |
| LCF Conversions Limited | Professional & scientific | 2020 |
| LE Papillon Ltd | Wholesale & retail | 2024 |
| Leadership Capital Ltd | Professional & scientific | 2015 |
| Leafstone Ltd | Construction | 2024 |
| Legacy Martial Arts Academy - Cheltenham Ltd | Arts & recreation | 2024 |
| Legacy Martial Arts Academy - Dursley Ltd | Arts & recreation | 2023 |
| Legacy Martial Arts Academy Ltd | Arts & recreation | 2022 |
| Legends of the Wayside Ltd | Information & communication | 2022 |
| Legion Leadership Ltd | Administrative & support | 2019 |
| Leigh DE COL Building & Renovations Limited | Construction | 2019 |
| Leola Bakery Limited | Hospitality | 2025 |
| Leonard Taylor & Sons (Butchers) Limited | Wholesale & retail | 1946 |
| Leonhard Lang MED Devices Ltd | Wholesale & retail | 2025 |
| Levallois Consulting Limited | Extraterritorial | 2010 |
| Leveret Music LLP | | 2019 |
| Leviathan Instruments Limited | Manufacturing | 2015 |
| Lewis and Wood (Holdings) Limited | Professional & scientific | 2015 |
| Lewis and Wood Limited | Wholesale & retail | 1995 |
| Lexical Analysis Software Ltd | Information & communication | 2004 |
| Lhbh Holdings Ltd | Professional & scientific | 2017 |
| Liam Ward Music Ltd | Education | 2023 |
| Liberty Stone Racing Ltd | Agriculture & fishing | 2025 |
| Lichen Studio Ltd | Professional & scientific | 2021 |
| Lief Financial Services Ltd | Financial services | 2003 |
| Lifeforce Organics Ltd | Manufacturing | 2018 |
| Lifestyle Bathrooms Ltd | Wholesale & retail | 2015 |
| Lighthouse Interactive Limited | Information & communication | 2015 |
| Ligneus Holdings Ltd | Professional & scientific | 2017 |
| Ligneus Products Limited | Manufacturing | 2013 |
| Ligneusplay Ltd | Manufacturing | 2021 |
| Lilena Care Home Cornwall Limited | Health & social care | 2018 |
| Limecharm Limited | Households | 1989 |
| Limina Consulting Ltd | Professional & scientific | 2025 |
| Limit Break Engineering Ltd | Manufacturing | 2021 |
| Limpet Lets Limited | Real estate | 2025 |
| Lindsay Pugh Limited | Administrative & support | 2012 |
| Linstock Lodge (Flat Management) Limited | Households | 1984 |
| Liro Services LLP | | 2008 |
| Lisa Oxenham Ltd | Information & communication | 2026 |
| Listeners Productions Ltd | Information & communication | 2024 |
| Little by Little Films Limited | Information & communication | 2011 |
| Little Fish Big Fish Ltd | Professional & scientific | 2022 |
| Little Ginger at Miserden Ltd | Hospitality | 2024 |
| Little Ginger Deli Limited | Hospitality | 2020 |
| Little MAN Project Limited | Professional & scientific | 2010 |
| Little Monster Limited | Administrative & support | 2010 |
| Live Full OUT Ltd | Administrative & support | 2020 |
| Live in Full Empowerment Ltd | Professional & scientific | 2023 |
| LIZ Marney Consulting Ltd | Professional & scientific | 2020 |
| LIZ Mynard Associates Limited | Professional & scientific | 2004 |
| Llarke Enterprises Ltd. | Professional & scientific | 2023 |
| Local Community Market Gardens CIC | Agriculture & fishing | 2022 |
| Lock Stock Apparel Ltd | Wholesale & retail | 2023 |
| Locks Mill Management Company Limited | Households | 2021 |
| Lofty Digital Ltd | Information & communication | 2025 |
| Logilink Technologies Limited | Information & communication | 1995 |
| Logistical Security Ltd | Other services | 2000 |
| London and Cheltenham Estates Limited | Real estate | 2001 |
| London Pierson Studios Limited | Information & communication | 2026 |
| London Road Hardware Limited | Wholesale & retail | 2011 |
| Longfield Hospice Care | Health & social care | 1988 |
| Longridge Partnership and Development Company Limited | Professional & scientific | 2009 |
| Longview Care Home Cornwall Limited | Health & social care | 2019 |
| Look Again Photography Limited | Professional & scientific | 2021 |
| LOS Cubanos Ltd | Hospitality | 2023 |
| Loss Gain Limited | Arts & recreation | 2021 |
| Lost and Found Creative Ltd | Professional & scientific | 2012 |
| Lotusv Market Ltd | Wholesale & retail | 2024 |
| Loudspeaker Technology Limited | Manufacturing | 1980 |
| Louisa Clarke Coaching Ltd | Professional & scientific | 2014 |
| Love Design Ltd | Professional & scientific | 2025 |
| Loveyourstove Chimney Sweep & Services Ltd | Administrative & support | 2018 |
| Lower Lutheredge Farm Limited | Agriculture & fishing | 1997 |
| Lower Street Atelier Ltd | Professional & scientific | 2023 |
| Lpte Services (Stroud) Ltd | Real estate | 2023 |
| LS & MS & Co Limited | Information & communication | 2024 |
| LSF Building and Renovations Limited | Construction | 2023 |
| Lucia Mcguinness Ltd | Professional & scientific | 2024 |
| Ludo Pals Limited | Manufacturing | 2025 |
| Ludus Capital Limited | Manufacturing | 2011 |
| Luminis Organisational Consulting Limited | Professional & scientific | 2010 |
| Luna Pet Healthcare Limited | Professional & scientific | 2024 |
| Lunar Film and Television Ltd | Information & communication | 2002 |
| Lwaquaticraffles Limited | Agriculture & fishing | 2025 |
| LYN Niblett Bookkeeping Services Limited | Professional & scientific | 2004 |
| Lynch Knoll Wind Park Limited | Energy | 1995 |
| Lypiatt Designs Limited | Arts & recreation | 1981 |
| Lypiatt Hill Lodgings LLP | | 2019 |