| Joanna Fallon Studio Ltd | Arts & recreation | 2016 |
| Joanna Nicolas Limited | Professional & scientific | 2010 |
| JOE Langston Ltd | Construction | 2011 |
| John R Mcgill Limited | Construction | 1984 |
| John Watson Education Ltd | Education | 2025 |
| Johnston Walsh Limited | Other services | 2004 |
| JON Close Consulting Limited | Professional & scientific | 2013 |
| Jonathan Bevan Design Ltd | Professional & scientific | 2012 |
| Jonathan Buckley Limited | Professional & scientific | 2013 |
| Jones Mellor Limited | Administrative & support | 2013 |
| Jonny Martin Projects Ltd | Manufacturing | 2025 |
| Jonti Telemarketing Ltd | Administrative & support | 2011 |
| Josephine Munsey Limited | Professional & scientific | 2021 |
| Joy Distribution Ltd | Health & social care | 2024 |
| JP Catering & Events Ltd | Hospitality | 2016 |
| Jpeg Carpentry Limited | Construction | 2016 |
| JRN Drylining Limited | Construction | 2026 |
| Jryc Ltd | Professional & scientific | 2023 |
| JSH Associates Limited | Financial services | 2010 |
| Jsre & Development Limited | Real estate | 2023 |
| Jt-Id Limited | Professional & scientific | 2004 |
| Julian Howes Wealth Management Limited | Financial services | 2011 |
| July Eighteen Ltd | Professional & scientific | 2019 |
| Junction 13 Limited | Hospitality | 2008 |
| Juniper Tree Properties Ltd | Construction | 2018 |
| Junk Baby Limited | Manufacturing | 2024 |
| Jupiter Care Holdings Ltd | Financial services | 2020 |
| Jupiter Care Ltd | Health & social care | 2000 |
| Just Chillin' Air Conditioning Ltd | Energy | 2019 |
| Just Ride the Bike Ltd | Professional & scientific | 2018 |
| Justin's MOT Centre Ltd | Wholesale & retail | 2020 |
| JVM Space Ltd | Information & communication | 2017 |
| JWP Creative Limited | Professional & scientific | 2014 |
| K & L Online Limited | Wholesale & retail | 2025 |
| K C Developments (Stroud) Limited | Construction | 2008 |
| K Yateman & Sons Limited | Construction | 2003 |
| K.J.S. Construction Limited | Construction | 2002 |
| Kaelix International Enterprise Limited | Wholesale & retail | 2026 |
| Kaizen People Ltd | Professional & scientific | 2023 |
| Kantaoui Catering Services Ltd | Administrative & support | 2024 |
| Kardinell Limited | Extraterritorial | 1990 |
| Karst Jewellery Limited | Wholesale & retail | 2019 |
| Kashmir & Oriental Textiles Ltd | Wholesale & retail | 2026 |
| Kate Danielson Associates Limited | Administrative & support | 2010 |
| Kate Snell Books and Films Ltd | Professional & scientific | 2020 |
| Katherine Colby Hydrologists Limited | Other services | 2016 |
| Kathryn Clark Limited | Administrative & support | 2007 |
| Katie Allen Consulting Limited | Professional & scientific | 2020 |
| Katie Rose Johnston Limited | Arts & recreation | 2016 |
| KEC Ultrasound Ltd | Health & social care | 2025 |
| Keen EYE Media Limited | Information & communication | 2017 |
| KELSOON100 Limited | Financial services | 2020 |
| Kemble MOT Centre Limited | Administrative & support | 2008 |
| Kensington House Management (Stroud) Limited | Households | 2018 |
| Kent-Baguley Ltd | Real estate | 2017 |
| Kepler IT Services Limited | Information & communication | 2020 |
| Kepros Ltd | Wholesale & retail | 2019 |
| Kerry Maintenance Limited | Construction | 2007 |
| Keydynamic Ltd | Professional & scientific | 2009 |
| Keynote Speaker Ltd | Education | 2016 |
| Keynsham Machinery Services Limited | Manufacturing | 2014 |
| Keystone Cotswold Limited | Construction | 2015 |
| KFA Limited | Professional & scientific | 2016 |
| Khadiza 1 Ltd | Hospitality | 2026 |
| KHH (Eastington) Ltd | Hospitality | 2021 |
| Kiblec Holdings Limited | Financial services | 2022 |
| Kiblec Limited | Construction | 2021 |
| Kick OFF Stroud Community Interest Company | Arts & recreation | 2012 |
| Kick-A-Bout Cirencester and Stroud Limited | Education | 2022 |
| Kick-A-Bout Kidz Limited | Education | 2012 |
| Kidrated Limited | Arts & recreation | 2013 |
| Kikic Holdings Ltd | Financial services | 2019 |
| Kind Comms Ltd | Professional & scientific | 2019 |
| Kings Lynn Wind Park Limited | Professional & scientific | 2006 |
| Kingsditch Properties Limited | Real estate | 1994 |
| Kitchen Garden Foods Limited | Manufacturing | 2003 |
| Kitchen Witch Cakery Ltd | Manufacturing | 2024 |
| Kitesnest Developments Limited | Real estate | 2017 |
| KM Advisory Services Limited | Professional & scientific | 2020 |
| KM Management and Consultancy Ltd | Professional & scientific | 2015 |
| KME Property Ltd | Real estate | 2012 |
| KMS Properties Cotswolds Ltd | Real estate | 2024 |
| Knapp Projects Ltd | Agriculture & fishing | 2022 |
| Known Research Ltd | Information & communication | 2023 |
| Koan Adventures Ltd | Information & communication | 2013 |
| Konsolt Ltd | Information & communication | 2015 |
| KPR Property Cotswold Ltd | Real estate | 2023 |
| Kudos Landscapes Ltd | Administrative & support | 2018 |
| Kyne Partners Ltd | Professional & scientific | 2026 |
| L Jensen Consultancy Ltd | Professional & scientific | 2024 |
| L Proctor Consulting Ltd | Professional & scientific | 2014 |
| L. Butler Plumbing and Heating Ltd | Construction | 2007 |
| L.A Motors Stroud Ltd | Wholesale & retail | 2016 |
| L.R. Motors Limited | Wholesale & retail | 2006 |
| L4 Enterprises CIC | Professional & scientific | 2013 |
| LA Gomera Property Limited | Households | 2010 |
| LA HEI Surgical Services Ltd | Health & social care | 2022 |
| Labour Market Initiatives Ltd | Education | 2025 |
| Labquip NDT Limited | Wholesale & retail | 2015 |
| Labs Curry Ltd | Manufacturing | 2015 |