| 1 Stop Fire and Security Ltd | Administrative & support | 2014 |
| 1-9 Truckwear Ltd | Wholesale & retail | 2021 |
| 101 Engineering Limited | Manufacturing | 1974 |
| 10A Ltd | Professional & scientific | 2015 |
| 12 Inch Media Ltd | Information & communication | 2017 |
| 12 Springfield Court (Stonehouse) Management Company Limited | Households | 2024 |
| 14 Kendrick Ltd | Real estate | 2017 |
| 177 Westward Road Flat Management Company Limited | Construction | 2016 |
| 18 Lower Bristol Road Management Company Limited | Households | 1991 |
| 1ST Call Auto Rentals Limited | Extraterritorial | 2019 |
| 1ST Call Commercial Properties Limited | Real estate | 2014 |
| 1ST Call Properties Limited | Construction | 2012 |
| 1ST Call Storage Limited | Administrative & support | 2014 |
| 1ST Call Stroud Limited | Administrative & support | 2010 |
| 1ST Call Warehousing Limited | Real estate | 2016 |
| 2 LET Glos Ltd | Real estate | 2007 |
| 22-48 Boakes Drive Management Company Limited | Households | 2017 |
| 360FREEDOM Limited | Professional & scientific | 2012 |
| 3C Business Associates Limited | Professional & scientific | 2003 |
| 4 Connected Limited | Professional & scientific | 2016 |
| 4 Ever TT Limited | Hospitality | 2017 |
| 41 Hindmans Road Ltd | Households | 2006 |
| 48 Hours Gold Limited | Wholesale & retail | 2018 |
| 4WARD Employment Services Limited | Administrative & support | 2018 |
| 5 Valleys Physio (Gloucester) LLP | | 2020 |
| 5 Valleys Physiotherapy (Gloucester) Limited | Health & social care | 2022 |
| 5 Valleys Physiotherapy (Stroud) Limited | Health & social care | 2022 |
| 7 PLX Ltd | Wholesale & retail | 2019 |
| 7 X 7 Electronic Design Ltd | Mining & quarrying | 2016 |
| 8 Caledonia Place (Management) Limited | Households | 1983 |
| 928 Specialists Ltd | Wholesale & retail | 2015 |
| A & R Motorcycle's Ltd | Wholesale & retail | 2010 |
| A & R Scaffolding (Cotswolds) Limited | Construction | 2001 |
| A Holcombe Ltd | Construction | 2022 |
| A I M (Stroud) Ltd | Construction | 2018 |
| A. J. F. Scott Limited | Manufacturing | 2003 |
| A. Wheeler & Sons Transport Limited | Transport & logistics | 2007 |
| A.J.T Butt Property Solutions Ltd | Real estate | 2025 |
| A.R.Daniels and Co.Limited | Wholesale & retail | 1959 |
| A1 Maintenance (Stroud) Limited | Construction | 1997 |
| A1 Motor Services (Stroud) Ltd | Wholesale & retail | 2015 |
| A2B Travel (Larkhall) Ltd | Transport & logistics | 2025 |
| AAE Installations Ltd | Construction | 2018 |
| Aatt Investments Limited | Financial services | 2019 |
| Abacus Finance Holdings Ltd | Financial services | 2018 |
| Abacus Finance Limited | Real estate | 1972 |
| Abacus Management Company (Romney) Limited | Real estate | 2018 |
| Abacus Upholstery Limited | Manufacturing | 2021 |
| Abi's 22 Limited | Hospitality | 2019 |
| Abintus Ltd | Administrative & support | 2015 |
| Abita Limited | Professional & scientific | 1997 |
| AC Comms Limited | Construction | 2024 |
| AC Electrical and Interiors Limited | Construction | 2017 |
| Accounting & Payroll Stationery Limited | Administrative & support | 2010 |
| Ace Wellbeing Ltd | Health & social care | 2016 |
| Active Schools Network Limited | Education | 2020 |
| Adam Griffiths Consulting Limited | Education | 2022 |
| Adams Fletcher Limited | Professional & scientific | 2002 |
| Adcal Professional Services Limited | Professional & scientific | 2016 |
| ADM Electrical Installations Ltd | Construction | 2023 |
| Adsb Systems Ltd | Professional & scientific | 2010 |
| Advaniti Associates Limited | Professional & scientific | 2024 |
| Advaniti Limited | Professional & scientific | 2007 |
| Aerocomp Limited | Financial services | 2004 |
| AG Creations Limited | Professional & scientific | 2020 |
| AG London Limited | Wholesale & retail | 2024 |
| Air Property Services Ltd | Construction | 2018 |
| AJ Estate Agents of Gloucestershire Limited | Real estate | 2020 |
| Alan Tyndall Limited | Wholesale & retail | 1984 |
| Alfie 007 Limited | Professional & scientific | 2021 |
| Alfresco Landscaping & Building Supplies Ltd | Wholesale & retail | 2021 |
| ALL Done & Dusted Cleaning Services Commercial Ltd | Administrative & support | 2006 |
| ALL Pulling Together Community Interest Company | Arts & recreation | 2017 |
| Allaction Limited | Professional & scientific | 2020 |
| Allan Webb Ltd | Information & communication | 1983 |
| Allen Accountants Limited | Professional & scientific | 2021 |
| Allen Bros (Cotswolds) Ltd | Construction | 2026 |
| Allen-White Limited | Other services | 2000 |
| Allotropix Limited | Professional & scientific | 2025 |
| Alverdiscott Battery Storage Limited | Energy | 2023 |
| Always Herd (Slimbridge) CIC | Health & social care | 2023 |
| Always Herd Ltd | Health & social care | 2022 |
| Amelore Limited | Professional & scientific | 2010 |
| Ammo Lifestyle Ltd | Wholesale & retail | 2022 |
| Amprov Trading Ltd | Wholesale & retail | 2011 |
| Anderson Norman Landscapes Limited | Administrative & support | 2016 |
| Andoversford Link Management Limited | Households | 1990 |
| Andreas Biermann Ltd | Hospitality | 2020 |
| Andrew Greenslade Ltd | Wholesale & retail | 2019 |
| Andrew J Frost Ltd | Information & communication | 2011 |
| Andrew J Montgomery Consultants Limited | Real estate | 2013 |
| Andy B'S Accountancy Limited | Professional & scientific | 2012 |
| Anella Brands Limited | Wholesale & retail | 2020 |
| Angus Kyloe Partners Limited | Information & communication | 2015 |
| Animara Studios Ltd | Information & communication | 2024 |
| Ansah Engineering Limited | Information & communication | 2022 |
| Anstee & Ware Group Limited | Construction | 2006 |
| Anstee & Ware Property Limited | Professional & scientific | 1998 |
| Anstee Coil Technology Limited | Manufacturing | 2014 |
| Ante Studio Limited | Professional & scientific | 2025 |