| Anthony J. Smith (Gloucester) Limited | Construction | 1976 |
| Antics Limited | Wholesale & retail | 1996 |
| Anvil Court (Dursley) Limited | Hospitality | 1994 |
| AOK Standish Ltd | Professional & scientific | 2023 |
| APB Alloys Limited | Extraterritorial | 2002 |
| Apis Holdings Limited | Professional & scientific | 2023 |
| Apollo Taxis Stonehouse Limited | Transport & logistics | 2024 |
| Apple Tree Leisure Homes Limited | Administrative & support | 2016 |
| Apple Tree Park Limited | Administrative & support | 2008 |
| Apple Tree Renewable Energy Consultants Ltd | Professional & scientific | 2024 |
| Aquatek Limited | Administrative & support | 2015 |
| Arboricultural Association Trading Limited | Agriculture & fishing | 2004 |
| ARC Construction Contracts Limited | Construction | 2017 |
| Archaeopress Publishing Limited | Information & communication | 2015 |
| Archiet Ltd | Information & communication | 2026 |
| Archisec Limited | Information & communication | 2015 |
| Arco Homes Limited | Construction | 2007 |
| Arcobuild (UK) Ltd | Manufacturing | 2008 |
| Arisam Limited | Information & communication | 2018 |
| Arlo Neal Ltd | Administrative & support | 2016 |
| Armentum Ltd | Information & communication | 2022 |
| Arnolds Cars Limited | Wholesale & retail | 2019 |
| Arrijan Farming Ltd | Agriculture & fishing | 2011 |
| Arthurs Yard Management Limited | Households | 2021 |
| Artiqus Ltd | Wholesale & retail | 2019 |
| AS Electrical Services (Stonehouse) Limited | Construction | 2014 |
| Ascari Services Ltd | Professional & scientific | 2026 |
| Ashgill Minimarket Limited | Wholesale & retail | 2020 |
| Ashley Garage Limited | Wholesale & retail | 2010 |
| Ashley Snell Bathrooms Limited | Construction | 2023 |
| Association for the Education and Guardianship of International Students | Other services | 1997 |
| Asteno Limited | Information & communication | 2012 |
| Astonmartinbits.Com Limited | Extraterritorial | 2018 |
| ATC Logistics Solutions Ltd | Transport & logistics | 2021 |
| Athean Ltd | Professional & scientific | 2025 |
| Atlas All-Stars Limited | Education | 2015 |
| Atlas Camps Limited | Health & social care | 2015 |
| Atlas Union Ltd | Wholesale & retail | 2023 |
| Atom ESS UK Limited | Wholesale & retail | 2024 |
| Aubiose Limited | Administrative & support | 2007 |
| Audio Books on Tape Limited | Extraterritorial | 2015 |
| August East Limited | Professional & scientific | 2006 |
| Auto Bodytech Limited | Wholesale & retail | 1987 |
| Auto Revive (South West) Ltd | Manufacturing | 2015 |
| Autocrafts Services Limited | Wholesale & retail | 1982 |
| Autofirex Limited | Public administration | 2018 |
| Axis Builders Ltd | Extraterritorial | 2015 |
| Ayyappan Ltd | Health & social care | 2025 |
| Azaaam Ltd | Transport & logistics | 2024 |
| B Line Project Services Limited | Construction | 2007 |
| B-Proud Property Developments Ltd | Construction | 2021 |
| B.S.M.A. | Arts & recreation | 1995 |
| Bailey Building Company Limited | Construction | 2009 |
| Balanced-Transitions Limited | Education | 2026 |
| Bandwidth Limited | Extraterritorial | 1998 |
| Batson Creek Marine Ltd | Transport & logistics | 2016 |
| Battri Solutions Limited | Professional & scientific | 2022 |
| Bbdgb Ltd | Other services | 2025 |
| BCB Marketing Ltd | Professional & scientific | 2023 |
| Beacon Electrical Limited | Construction | 2005 |
| Bear Barbers Ltd | Other services | 2023 |
| Beasty Baps Limited | Hospitality | 2025 |
| Beavers Staffing Solutions Limited | Administrative & support | 2020 |
| Beavers Support Services Limited | Health & social care | 2009 |
| Bee's Knees Building & Landscaping Ltd | Administrative & support | 2024 |
| BEN Wright Limited | Wholesale & retail | 2014 |
| Benchbook Ltd | Professional & scientific | 2026 |
| Bennett Construction (U.K.) Limited | Construction | 1990 |
| Berkdale-James Limited | Information & communication | 2024 |
| Bespoke Garden Buildings Limited | Construction | 2024 |
| Best Friends Day Nurseries Ltd | Other services | 2014 |
| Better Together Conditioning Ltd | Arts & recreation | 2023 |
| Bitservuk Limited | Information & communication | 2011 |
| Black Triangle Associates Limited | Professional & scientific | 2026 |
| Bliss Domus Limited | Real estate | 2025 |
| Blue Snail Films Limited | Arts & recreation | 2021 |
| Blueprint Project Solutions Ltd | Professional & scientific | 2011 |
| BMC 2019 Limited | Wholesale & retail | 2019 |
| Bobcat IT Limited | Information & communication | 2006 |
| Bonds Mill Cottages Management Limited | Agriculture & fishing | 2005 |
| Borough Consultancy Services Limited | Professional & scientific | 2018 |
| Bosco Brothers Limited | Wholesale & retail | 2011 |
| Bosh Farming Ltd | Agriculture & fishing | 2021 |
| Bowshiel Solar Ltd | Energy | 2024 |
| Bramleys Greengrocers Limited | Administrative & support | 2008 |
| Brandspeak Limited | Professional & scientific | 2016 |
| Brave New World Holdings Ltd | Information & communication | 2022 |
| Brelles Care Solutions Limited | Administrative & support | 2023 |
| Bridge Football Academy Limited | Education | 2019 |
| Bridgend Garage Bridgend Glos Ltd | Wholesale & retail | 2017 |
| Bristol Lime and Conservation Limited | Construction | 2014 |
| Brit Lanka Tours Ltd | Administrative & support | 2026 |
| Bronzeleaf Group Limited | Agriculture & fishing | 2019 |
| Bronzeleaf Limited | Administrative & support | 2001 |
| Bronzeleaf Rail Limited | Agriculture & fishing | 2015 |
| Brunel Court (Stroudwater) Limited | Real estate | 2000 |
| Brunel Homes (Gloucester) Ltd | Construction | 2013 |
| BSB Bookkeeping and Accountancy Ltd | Information & communication | 2019 |
| BSS Enterprises Ltd | Real estate | 2023 |
| Building Solutions Landscaping Ltd | Administrative & support | 2025 |