| ER7 Ltd | Transport & logistics | 2025 |
| Erciyes Grill Ltd | Hospitality | 2026 |
| ERE Solutions Ltd | Information & communication | 2024 |
| Ereach Trading Ltd | Wholesale & retail | 2014 |
| Ergorite Industry Co., Ltd. | Extraterritorial | 2015 |
| Eric Industry Company Limited | Wholesale & retail | 2005 |
| Erratic Holdings Ltd | Real estate | 2018 |
| Erwin Hymer Centre (Travelworld) Limited | Administrative & support | 2016 |
| ES Harris Group Ltd | Construction | 2020 |
| ES Travel Ltd | Administrative & support | 2025 |
| Esanef Ltd | Arts & recreation | 2021 |
| Esarjun Education Ltd | Education | 2022 |
| Esarjun Food Ltd | Hospitality | 2020 |
| Escalon Investments Ltd | Real estate | 2022 |
| Escalon Medical Services Limited | Health & social care | 2013 |
| Escape Nation Ltd | Arts & recreation | 2017 |
| ESE Contracts Ltd | Agriculture & fishing | 2012 |
| Espee Property Maintenance Limited | Construction | 2024 |
| Espire Construction Limited | Other services | 2016 |
| Espkpoth Limited | Wholesale & retail | 2025 |
| Espley Holdings Limited | Construction | 2019 |
| ESS Energy Group Limited | Administrative & support | 2011 |
| Essential Clothing Company Group Limited | Real estate | 1996 |
| Essential Hair & Beauty Limited | Other services | 2004 |
| Essential Lifestyle Products Limited | Wholesale & retail | 2022 |
| Essential Parenting Ltd | Health & social care | 2019 |
| Essential Training & Consultancy Company Ltd | Education | 2021 |
| Essington Construction Limited | Construction | 1997 |
| Esss Limited | Energy | 2017 |
| Estate Property Solutions Limited | Construction | 2014 |
| Esteem Educators Staffordshire Ltd | Education | 2025 |
| Etchells Presses Limited | Manufacturing | 2004 |
| Eternity Smile Ltd | Wholesale & retail | 2023 |
| Ethan Trading Company Limited | Wholesale & retail | 2024 |
| Ethereal Nest Limited | Wholesale & retail | 2025 |
| Ethical Horsemanship Limited | Administrative & support | 2017 |
| Ets-Makadji & Frere Trading Co., Limited | Wholesale & retail | 2025 |
| EU Automation America Ltd | Administrative & support | 2016 |
| EU Automation Asia Pacific Ltd | Administrative & support | 2016 |
| EU Automation International Ltd | Information & communication | 2016 |
| European Automation Limited | Information & communication | 2009 |
| Eutextikon (Computational Materials Consultancy) Limited | Information & communication | 2016 |
| EVA Cartwright Consulting Ltd | Professional & scientific | 2023 |
| Evacrombe Health Ltd | Wholesale & retail | 2023 |
| Evans ASH Financial Services LLP | | 2005 |
| Evaris Care Ltd | Health & social care | 2025 |
| Eveleen Trading Limited | Wholesale & retail | 2025 |
| Evendove Services Limited | Professional & scientific | 1990 |
| Ever Thine Film & Photography Ltd | Professional & scientific | 2023 |
| Everest Grocery Store Ltd | Wholesale & retail | 2015 |
| Everest Retail Ltd | Wholesale & retail | 2024 |
| Everflow Global PTE Ltd | Wholesale & retail | 2026 |
| Evergreen HR Limited | Professional & scientific | 2015 |
| Evergreen ST19 Limited | Hospitality | 2018 |
| Everva Ltd | Wholesale & retail | 2025 |
| Every Silver Cloud Ltd | Health & social care | 2017 |
| Every Soul Counts Limited | Wholesale & retail | 2021 |
| Everything Boutique Holdings Limited | Financial services | 2025 |
| Evmu Logistics Ltd | Transport & logistics | 2025 |
| EVO Property Limited | Real estate | 2016 |
| Evode Limited | Professional & scientific | 1932 |
| Evohr Solutions Ltd | Administrative & support | 2024 |
| Evolve Haulage Limited | Transport & logistics | 2020 |
| Evoque Travel Concierge Ltd | Administrative & support | 2021 |
| Evsar Contruction Ltd | Construction | 2022 |
| EVW Grooming Limited | Other services | 2015 |
| EX SL Transfers Midlands Limited | Education | 2024 |
| EX Systems Limited | Extraterritorial | 2015 |
| Exact Engineering Limited | Construction | 2021 |
| Excellent Health Care Ltd | Health & social care | 2025 |
| Excelsior Foods Limited | Hospitality | 2024 |
| Exloy Trading Limited | Wholesale & retail | 2025 |
| Expert Exporter Trading Co., Ltd | Wholesale & retail | 2024 |
| Express Curtains Limited | Manufacturing | 2003 |
| Extended Care Ltd | Health & social care | 2020 |
| Extme Trading Limited | Wholesale & retail | 2025 |
| Extra Mile Healthcare Ltd | Health & social care | 2024 |
| Extramile Supported Living Care Services Ltd | Health & social care | 2026 |
| Extreme Transport Ltd | Transport & logistics | 2013 |
| Exuberant Rafal Ltd | Wholesale & retail | 2023 |
| Eye-Level Optical Limited | Health & social care | 1991 |
| Eyenouhs Ltd | Wholesale & retail | 2025 |
| Ezili Limited | Manufacturing | 2019 |
| Ezzray Ltd | Wholesale & retail | 2026 |
| F Best Hand Car Wash Ltd | Administrative & support | 2026 |
| F Blaize Plumbing Heating Pipework Limited | Construction | 2007 |
| F M Roofing Services Limited | Construction | 2021 |
| F M S Car & Truck Limited | Wholesale & retail | 2003 |
| F&F Mart Limited | Information & communication | 2019 |
| F&M Tech Ltd | Wholesale & retail | 2023 |
| F&N Haulage Ltd | Transport & logistics | 2023 |
| F. Espley & Sons Limited | Construction | 1976 |
| F3 Technologies U.K. Limited | Information & communication | 2023 |
| Face in a Crowd Limited | Arts & recreation | 2013 |
| Faces Consent Limited | Information & communication | 2020 |
| Faces Pharmacy Limited | Wholesale & retail | 2024 |
| Faces Training Academy Limited | Education | 2025 |
| Faction Agency Ltd | Wholesale & retail | 2018 |
| Fady International Trading Company Limited | Wholesale & retail | 2026 |
| FAI KAM UK Ltd | Manufacturing | 2025 |