| Dupont Lade Technology Co., Ltd | Professional & scientific | 2021 |
| Durrell-Wolf Ltd | Wholesale & retail | 2023 |
| Dust Demons (Stafford) Limited | Other services | 2010 |
| Dust Demons Midlands Limited | Administrative & support | 2016 |
| Dust Demons UK Limited | Administrative & support | 2016 |
| Dusta Cleaning Limited | Administrative & support | 2024 |
| DVS Quantity Surveying Ltd | Professional & scientific | 2009 |
| DW Consultancy (UK) Ltd | Administrative & support | 2010 |
| DW Parcel Pilot Limited | Transport & logistics | 2025 |
| DW Plumbing & Heating Limited | Construction | 2004 |
| DWL Partners Limited | Information & communication | 2021 |
| DWS Consulting Ltd | Extraterritorial | 2019 |
| DY8 Project Management Limited | Professional & scientific | 2012 |
| Dymaynea Limited | Wholesale & retail | 2025 |
| Dynamic Air Systems Ltd | Wholesale & retail | 2013 |
| Dynamic Spark Limited | Wholesale & retail | 2024 |
| Dynamiq Asset Holdings Limited | Transport & logistics | 2023 |
| Dynastat Podiatry Ltd | Health & social care | 2006 |
| Développé Holdings Limited | Real estate | 2021 |
| E & S Mears Ltd | Wholesale & retail | 2011 |
| E G Truck Services Limited | Wholesale & retail | 2011 |
| E Green & SON Limited | Manufacturing | 2006 |
| E Harris (Transport) Ltd | Transport & logistics | 2016 |
| E L Flooring Limited | Construction | 2018 |
| E&A Transport Ltd | Transport & logistics | 2019 |
| E&M Music Ltd | Information & communication | 2025 |
| E-Soil Limited | Extraterritorial | 2020 |
| E-Van Guru Limited | Wholesale & retail | 2020 |
| E.W. Humphries Limited | Real estate | 1950 |
| E5 PUB Co Ltd | Hospitality | 2011 |
| EA Bars & Events Ltd | Financial services | 2024 |
| Eagle Car Hire Limited | Transport & logistics | 2022 |
| Eagle EYE Consult Ltd | Real estate | 2020 |
| Eagle EYE Developments Limited | Professional & scientific | 2015 |
| Eaglesong Crown United Investment International Co., Ltd. | Wholesale & retail | 2025 |
| Earlycare Ltd | Health & social care | 2025 |
| Earth Flowers Ltd | Wholesale & retail | 2023 |
| Easpol International Logistics Co., Ltd | Wholesale & retail | 2023 |
| East Africa Services Ltd | Wholesale & retail | 2022 |
| East TV Ltd | Information & communication | 2007 |
| Eastar Trading Co., Ltd | Wholesale & retail | 2024 |
| Eastgate Chambers Limited | Professional & scientific | 2010 |
| Eastmoor Properties Streetly Ltd. | Households | 1991 |
| Easy Living Mobility Franchise Limited | Administrative & support | 2018 |
| Easy Living Mobility Limited | Wholesale & retail | 2017 |
| Easy Living Retail Ltd | Wholesale & retail | 2011 |
| Easy Policy Ltd | Information & communication | 2026 |
| Easysteel China Co., Ltd | Manufacturing | 2023 |
| Easytrade Import and Export Co., Ltd | Extraterritorial | 2020 |
| Eaton Richards Limited | Administrative & support | 1998 |
| Ebladez Limited | Wholesale & retail | 2009 |
| EC Data Services Ltd | Professional & scientific | 2021 |
| EC Skin & Beauty Ltd | Other services | 2025 |
| Ecas 2CV Parts Limited | Wholesale & retail | 2005 |
| Ecc-Yb Ltd | Transport & logistics | 2023 |
| Eccleshall Castle Farms Limited | Agriculture & fishing | 1987 |
| Eccleshall Dermatology Clinic Ltd | Health & social care | 2023 |
| Eccleshall Plant Services Limited | Wholesale & retail | 2008 |
| Eccleshall Show Society | Arts & recreation | 2008 |
| Echen Ltd | Manufacturing | 2021 |
| Echo WCS Limited | Administrative & support | 2022 |
| Echoa Ultrasound Ltd | Health & social care | 2025 |
| Eco Smart Technology Limited | Extraterritorial | 2021 |
| Ecomile Trading Limited | Other services | 2015 |
| Econatura Limited | Wholesale & retail | 2025 |
| Ecoo Display Limited | Wholesale & retail | 2015 |
| Ecosmartgen Power Systems Limited | Manufacturing | 2024 |
| Ecosos (UK) Limited | Professional & scientific | 2019 |
| Ecwrio S.P.A. Ltd | Mining & quarrying | 2022 |
| ED Stafford Ltd | Information & communication | 2011 |
| ED Weetman (Haulage and Storage) Limited | Transport & logistics | 1984 |
| ED Weetman (Haulage Contracts) Limited | Transport & logistics | 1986 |
| ED Weetman (North West) Limited | Transport & logistics | 1986 |
| ED Weetman (Properties) Limited | Professional & scientific | 1995 |
| Edbros Holding Company Ltd | Financial services | 2024 |
| Eden Star Properties Ltd | Construction | 2011 |
| Eden Valley Property Limited | Construction | 2019 |
| Edge Planning & Design Ltd | Professional & scientific | 2018 |
| Edinborough & Edwards PUB Company Limited | Hospitality | 2024 |
| Edison 1965 Limited | Extraterritorial | 2014 |
| Edith Thomas Limited | Health & social care | 2015 |
| EDS Business Consulting Ltd | Wholesale & retail | 2021 |
| Edson Evers EOT Limited | Professional & scientific | 2020 |
| Edson Evers Public Relations Limited | Professional & scientific | 2011 |
| Edward Court (Walsall) Limited | Households | 2025 |
| Edwards Houseshares Limited | Real estate | 2021 |
| EF Murray Ltd | Health & social care | 2014 |
| EFC Fencing LLP | | 2015 |
| Efficient Home Improvements Ltd | Construction | 2024 |
| Egregie Ltd | Professional & scientific | 2022 |
| Ejexpresscart UK Ltd | Wholesale & retail | 2025 |
| EJI Styling Ltd | Other services | 2020 |
| EJJ Holdings Limited | Financial services | 2025 |
| EKO Health Safety & Fire Consultancy Limited | Professional & scientific | 2025 |
| Ekotec Limited | Professional & scientific | 2003 |
| El-Assiie Trading Limited | Wholesale & retail | 2023 |
| Elalfy Medical Limited | Health & social care | 2017 |
| Elara Consulting Ltd | Professional & scientific | 2026 |
| Elastic Heritage Limited | Hospitality | 2022 |
| Elbury Health Limited | Education | 2019 |