| E.O.G.B. Energy Products Group Limited | Financial services | 2025 |
| E.W. Johnson Limited | Construction | 2003 |
| EAG Competitions Limited | Wholesale & retail | 2023 |
| Eagle Heating & Plumbing Limited | Construction | 1973 |
| East Court Management ST. Neots 1994 Limited | Households | 1994 |
| East Electrical Contractors Limited | Professional & scientific | 2010 |
| Easy Aggregates & Building Supply’S Ltd | Construction | 2025 |
| Easy Car Parts Ltd | Wholesale & retail | 2012 |
| Eaton Ford Osteopathy Ltd | Health & social care | 2025 |
| Eaton Plaice Limited | Hospitality | 2020 |
| Eaton Socon Engineering Limited | Manufacturing | 2003 |
| Eaton Socon Ex-Services Club Ltd | Hospitality | 2025 |
| Ebisan Legacy Properties Limited | Real estate | 2022 |
| Ebonyke Ltd | Health & social care | 2021 |
| Eccor Ltd | Professional & scientific | 2011 |
| Echo Data Resilience Limited | Information & communication | 2006 |
| Eco Baughan 2000 Ltd. | Manufacturing | 2001 |
| Ecological Farm Product Group Limited | Agriculture & fishing | 2017 |
| Edataworks Ltd | Administrative & support | 2019 |
| Eden Psychology & Consultancy Services Ltd | Education | 2020 |
| Edenvale Recruitment Limited | Administrative & support | 2021 |
| Edge Design & Marketing Ltd | Professional & scientific | 1973 |
| Ediasd Limited | Professional & scientific | 2024 |
| Edwards and YOU Marketing Limited | Administrative & support | 2009 |
| Effortless Foreign Exchange Ltd | Financial services | 2023 |
| EFS Forwarding Ltd | Administrative & support | 2015 |
| EFS Property Management Limited | Real estate | 2025 |
| EFS Property Services Ltd | Real estate | 2020 |
| EFS Safe Rentals Limited | Real estate | 2012 |
| EGG & Poultry Industry Conference Ltd | Other services | 1966 |
| Eidolon Tokenag Ltd | Financial services | 2025 |
| EJB Installations Limited | Manufacturing | 2024 |
| EJM Wine Ltd | Wholesale & retail | 2020 |
| Elandy Advisory Limited | Professional & scientific | 2024 |
| Elcenn Ltd | Administrative & support | 2023 |
| Eldense Ltd | Other services | 2023 |
| Electric Cat Productions Ltd | Professional & scientific | 2022 |
| Electric Evolution Limited | Construction | 2012 |
| Electro Controls Limited | Extraterritorial | 1980 |
| Electrovolt Electrical Services Limited | Construction | 2023 |
| Elerim Ltd | Other services | 2023 |
| Elite Tiling Solutions Ltd | Construction | 2024 |
| Ella 1964 Ltd | Extraterritorial | 2023 |
| Ellington Steam Limited | Administrative & support | 2019 |
| Elwood Environmental Ltd | Water & waste | 2025 |
| Emaglam Ltd | Other services | 2022 |
| Embc Solutions Ltd | Professional & scientific | 2013 |
| Emenay Financial Crime Consultants Limited | Information & communication | 2017 |
| Emery Ceramics Ltd. | Construction | 2005 |
| Emma Featherstone Writing and Editing Services Limited | Information & communication | 2018 |
| Emmit Limited | Manufacturing | 2019 |
| Emtss Ltd | Construction | 2017 |
| ENA Designs Limited | Information & communication | 2013 |
| Enable Smart Ltd. | Information & communication | 2009 |
| Energise Ltd | Professional & scientific | 2008 |
| Energy Innovation by Design Limited | Construction | 2023 |
| Engage Safety Ltd | Professional & scientific | 2015 |
| Ensa Property Ltd | Extraterritorial | 2024 |
| Ensignum Ltd | Construction | 2021 |
| Entertainment Show Productions Ltd | Arts & recreation | 2019 |
| Enzymatic Ltd | Wholesale & retail | 2025 |
| Epitome Development Ltd. | Construction | 2019 |
| Equine Vision Ltd | Information & communication | 2015 |
| Equity and Chill Ltd | Real estate | 2025 |
| Equiworld Limited | Wholesale & retail | 2010 |
| Erifire Safety Ltd | Public administration | 2024 |
| Escapeworks Holdings Ltd | Financial services | 2024 |
| ESO Consultancy Ltd | Information & communication | 2015 |
| ESP Designs Limited | Professional & scientific | 2018 |
| Etaxes Ltd | Professional & scientific | 2019 |
| Etch Tech Limited | Manufacturing | 2009 |
| Eternal Mycology Ltd | Wholesale & retail | 2023 |
| Etheo Tech Limited | Information & communication | 2025 |
| EVA Ready BBQ Ltd | Hospitality | 2022 |
| Evebus Ltd | Transport & logistics | 2013 |
| Evebus Tours Ltd | Administrative & support | 2016 |
| Events by Lily Ltd | Hospitality | 2025 |
| Ever Forward Learning Ltd | Education | 2025 |
| Everig Ltd | Other services | 2018 |
| Every Little Thing Ltd | Extraterritorial | 2015 |
| Everything IS Possible Ltd | Wholesale & retail | 2025 |
| Evetine Limited | Construction | 1997 |
| Evidential Marketing Ltd. | Administrative & support | 2016 |
| Evoworks Limited | Information & communication | 2000 |
| Excelsior Windows Limited | Construction | 2013 |
| Eximius Global Solutions Group Ltd | Professional & scientific | 2021 |
| Expeditions for Everyone C.I.C. | Education | 2024 |
| Expert Computers Limited | Information & communication | 2009 |
| Eynesbury Road Sweepers Ltd | Extraterritorial | 2024 |
| Eynesbury Transport Ltd | Extraterritorial | 2024 |
| F&M Hotspot Ltd | Hospitality | 2023 |
| Fabtic Ltd | Education | 2013 |
| Fadare&Co Limited | Administrative & support | 2014 |
| Fahrii Ltd | Other services | 2024 |
| Fairytale Marquees Limited | Other services | 2020 |
| Falcon Books Publishing Ltd | Information & communication | 2018 |
| Falvey Services Limited | Administrative & support | 2018 |
| Fantech Commissioning Limited | Construction | 2007 |
| Faristey Ltd | Other services | 2015 |
| Farrier (ST Neots) Management Limited | Households | 2021 |