| Dppt Logistic Limited | Transport & logistics | 2026 |
| DR Emma Woodward Ltd | Health & social care | 2026 |
| DR Jalal Ltd | Health & social care | 2025 |
| DR Jalal Property Holdings Ltd | Real estate | 2026 |
| DR Johnsen Limited | Health & social care | 2013 |
| Drevv Ltd | Information & communication | 2026 |
| Drib Okoto Global Limited | Health & social care | 2023 |
| Driftwood 8 Ltd | Real estate | 2025 |
| Driftwood DM Ltd | Information & communication | 2026 |
| Drive & Shine Ltd | Transport & logistics | 2023 |
| Driven Mobility Group Ltd | Transport & logistics | 2025 |
| Driving-Pro Limited | Education | 2021 |
| Drop Crown Ltd | Information & communication | 2019 |
| Durnford Court Freehold Limited | Households | 2014 |
| Durnford Court RTM Company Limited | Households | 2010 |
| Dyes Garage Ltd | Wholesale & retail | 2026 |
| Dynamic Decorating Ltd | Construction | 2025 |
| Dynix Ltd | Information & communication | 2025 |
| Dyslexia Portsmouth Limited | Education | 2019 |
| E Property Marketing Ltd | Information & communication | 2019 |
| E&O Developments Limited | Real estate | 2024 |
| E&S Pro Cleaning Ltd | Administrative & support | 2025 |
| E-Lec Solutions Ltd | Construction | 2012 |
| E-Storefront Global Limited | Wholesale & retail | 2025 |
| E3R SW Training Limited | Health & social care | 2014 |
| E4COMMERCIAL Electrics Limited | Construction | 2015 |
| Eagle I Services Ltd | Professional & scientific | 2024 |
| Earth Organic Limited | Administrative & support | 2023 |
| Earthbound Memorials Ltd | Other services | 2025 |
| East Hants Homes Limited | Real estate | 2025 |
| East Sung Limited | Hospitality | 2019 |
| East Wing Development Limited | Construction | 2021 |
| East Wing Holdings Limited | Financial services | 2021 |
| Eastbury Manor LLP | | 2023 |
| Eastern European Community in Portsmouth CIC | Health & social care | 2025 |
| Eastfields RTM Company Limited | Households | 2007 |
| Eastney Convenience Store Limited | Wholesale & retail | 2017 |
| Eaststar Properties (Southern) Limited | Construction | 2017 |
| Eastwest Goods Ltd | Wholesale & retail | 2022 |
| Easypen Limited | Health & social care | 2024 |
| Ebeneza Healthcare Plus Limited | Health & social care | 2016 |
| Eclipse Fundraising Ltd | Other services | 2020 |
| Eclipse Kitchen Ltd | Hospitality | 2025 |
| Eco Autoclean Ltd | Administrative & support | 2019 |
| Ecobuild Materials Ltd | Manufacturing | 2025 |
| Ecofit Office Interiors Ltd | Construction | 2010 |
| Ecogif Ltd | Wholesale & retail | 2020 |
| Ecowipeconsulting Ltd | Administrative & support | 2025 |
| Eden Home Improvements Ltd | Construction | 2025 |
| Edencom Ltd | Construction | 2023 |
| Edge Talent Consulting Ltd | Administrative & support | 2025 |
| Edit Laser Specialists Ltd | Other services | 2021 |
| Edmonds Learning and Development (El&D) Limited | Education | 2022 |
| Edward Frank Estates Limited | Real estate | 2026 |
| EF Orthodontics Limited | Health & social care | 2024 |
| Efex Hamsphire Domestic Cleaning Services Ltd | Administrative & support | 2023 |
| EFT Consultancy Services Limited | Financial services | 2013 |
| Eighty Eight Live Limited | Professional & scientific | 2018 |
| Eixlq Process Solutions Ltd | Professional & scientific | 2025 |
| EJH CS Limited | Information & communication | 2025 |
| Ekram Property Limited | Real estate | 2025 |
| EL Greco Limited | Hospitality | 2021 |
| Elaris Builds Ltd | Construction | 2025 |
| Elbre Ltd | Construction | 2016 |
| Electricity & Plumbing Services Ltd | Construction | 2022 |
| Electrotonic Limited | Information & communication | 2023 |
| Elephant in Scarlet CIC | Information & communication | 2015 |
| Elevata Consultancy Ltd | Professional & scientific | 2024 |
| Elham Khan Ltd | Hospitality | 2026 |
| Elite Aesthetics Portsmouth Limited | Other services | 2025 |
| Elite Clinical Academy Ltd | Education | 2025 |
| Elite Rooms Portsmouth Ltd | Real estate | 2017 |
| Elizabeth Livingstone Limited | Households | 2025 |
| Ellcarnivore Ltd | Wholesale & retail | 2026 |
| Elliott Building Consultants Ltd. | Construction | 2012 |
| Ellwood Management Limited | Households | 1999 |
| ELM Grove Enterprises Ltd | Hospitality | 2022 |
| ELM Grove Laundrycare Limited | Other services | 1996 |
| Elphinstone Apartments Management Ltd | Administrative & support | 2011 |
| Emirati Abaya Ltd | Wholesale & retail | 2023 |
| Emitter Ltd | Information & communication | 2024 |
| Emmbee Limited | Wholesale & retail | 2012 |
| Emmerson Environmental (South) Ltd | Construction | 2018 |
| Emsworth Spa Boutique Limited | Other services | 2022 |
| Encounter Christ Limited | Health & social care | 2024 |
| Enebral Holdings Limited | Financial services | 2018 |
| Energy & Safety Group Ltd | Financial services | 2023 |
| Energy Cert Solutions Ltd | Other services | 2023 |
| Engineering and Trading Limited | Manufacturing | 2014 |
| Enhanced by Jade Limited | Other services | 2021 |
| Enhanced Estates Ltd | Real estate | 2024 |
| Enpact Limited | Professional & scientific | 2019 |
| EO Inc Ltd | Financial services | 2024 |
| Epash Ltd | Wholesale & retail | 2025 |
| Equal Roots Arboriculture C.I.C. | Professional & scientific | 2026 |
| ERA Portsmouth Ltd | Real estate | 2019 |
| Ergo-Tech Ltd | Wholesale & retail | 2017 |
| Ersatz Limited | Wholesale & retail | 2025 |
| Escape Unclouded Ltd | Administrative & support | 2025 |
| Essentuals Ltd | Other services | 2025 |