| Dzisey Healthcare Limited | Health & social care | 2019 |
| Dzumi Ltd | Wholesale & retail | 2019 |
| Dérive Apparel UK Limited | Wholesale & retail | 2025 |
| E & F Homes Ltd | Real estate | 2017 |
| E & M Print Services Limited | Manufacturing | 2009 |
| E & R Brickwork Limited | Construction | 2017 |
| E and H Property Investments Ltd | Real estate | 2016 |
| E Bhakar Catering Limited | Hospitality | 2024 |
| E Clark Properties Limited | Administrative & support | 2010 |
| E Irons GAS, Plumbing and Heating Ltd | Construction | 2015 |
| E J Jenkinson Ltd | Real estate | 2018 |
| E J Plant Ltd | Construction | 2015 |
| E M Laboratories Limited | Administrative & support | 2008 |
| E Tea Limited | Hospitality | 2023 |
| E&B Plastering & Drylining Ltd | Construction | 2022 |
| E&E Properties & Investments Ltd | Real estate | 2025 |
| E&M Carpentry Limited | Construction | 2023 |
| E&S Ballkan Limited | Hospitality | 2026 |
| E- Transfers Ltd | Other services | 2010 |
| E-Bate Limited | Information & communication | 2017 |
| E-Bikes R&R Ltd | Manufacturing | 2026 |
| E-Investments Ltd | Wholesale & retail | 2016 |
| E-Powa Ltd | Administrative & support | 2017 |
| E-Shopspot Limited | Wholesale & retail | 2024 |
| E. a. Shipcare Limited | Energy | 1995 |
| E. M. Mortgages Ltd | Financial services | 2015 |
| E. Massey (Transport) Limited | Transport & logistics | 1976 |
| E. Williams Landscapes Ltd | Extraterritorial | 2021 |
| E. Williams Ltd | Administrative & support | 2011 |
| E.& J.Jarvis Limited | Wholesale & retail | 1963 |
| E.L.M Groundworks Ltd | Construction | 2022 |
| E.P.C. Marine Automation Limited | Administrative & support | 1980 |
| E.P.P Construction Specialist Ltd | Construction | 2025 |
| E101 HUB5 Ltd | Professional & scientific | 2023 |
| E3 Consulting (PTS) Limited | Professional & scientific | 2012 |
| E3 Consulting (VAT) Limited | Professional & scientific | 2012 |
| E3 Consulting Limited | Professional & scientific | 2003 |
| EA Holdings Group Limited | Administrative & support | 2022 |
| EA Property Group Ltd | Real estate | 2022 |
| EAA Properties Limited | Real estate | 2024 |
| EAG (Southern) Limited | Real estate | 2026 |
| Eagle Approach Limited | Information & communication | 2011 |
| Eagle Eyes UK. Ltd | Administrative & support | 2017 |
| Eagle Specialist Vehicles Limited | Manufacturing | 1981 |
| Eagle Tree Modular Limited | Construction | 2019 |
| Eagles WING01 Ltd | Health & social care | 2021 |
| Eaglestar Assets Management Limited | Administrative & support | 2005 |
| Eamonart Ltd | Arts & recreation | 2024 |
| EAR EAR WAX Removal Ltd | Health & social care | 2025 |
| EAR SAY Limited | Health & social care | 2022 |
| Earl Skateboards Ltd | Wholesale & retail | 2024 |
| Earlcourt Limited | Other services | 2025 |
| Early Years Advocacy Limited | Professional & scientific | 2015 |
| Earlystarts Childcare Ltd | Health & social care | 2022 |
| Earned Value Limited | Professional & scientific | 2014 |
| Earpal Limited | Health & social care | 2010 |
| Earpeace Limited | Wholesale & retail | 2016 |
| Earth Data Limited | Manufacturing | 1980 |
| Earth Fluid Solutions Limited | Professional & scientific | 2020 |
| Earth Power Ltd | Wholesale & retail | 2018 |
| Earth Rating Limited | Information & communication | 2022 |
| Earthrise Consulting Limited | Information & communication | 1996 |
| Earthstars Universal Enterprises Limited | Information & communication | 2007 |
| Earthstorm Media Limited | Information & communication | 2009 |
| East Bay Associates Ltd | Wholesale & retail | 2022 |
| East MID Civils Limited | Construction | 2024 |
| East Street Gardens Management Limited | Real estate | 2022 |
| East Sussex Roofing Contractors Ltd | Construction | 2015 |
| Eastbourne Estates Limited | Real estate | 2018 |
| Eastbury Court (Mudeford) Limited | Households | 1987 |
| Eastern House (2017) Ltd | Hospitality | 2017 |
| Eastern Transport and General Suppliers Ltd | Transport & logistics | 2024 |
| Eastern Valleys Ltd | Wholesale & retail | 2025 |
| Eastfield Commercial Limited | Real estate | 2021 |
| Eastfield Finance Group Limited | Financial services | 2025 |
| Eastgate (Winchester) Ltd | Real estate | 2022 |
| Eastgate Roofing Services Ltd | Construction | 2007 |
| Eastlake Engines Ltd | Wholesale & retail | 2006 |
| Eastlake Global Solutions Limited | Administrative & support | 2007 |
| Eastleigh Medical Practice Ltd | Health & social care | 2024 |
| Eastleigh Pride CIC | Arts & recreation | 2019 |
| Eastleigh Railway Preservation Society Ltd | Manufacturing | 1995 |
| Eastleigh Southern Parishes Network Limited | Health & social care | 2014 |
| Eastleigh Veterans' Centre CIC | Health & social care | 2025 |
| Easton Grace Properties Ltd | Extraterritorial | 2026 |
| Easton Residential Limited | Real estate | 2007 |
| Easy Care Systems Limited | Wholesale & retail | 2004 |
| Easy Cruise Parking Ltd | Transport & logistics | 2025 |
| Easy Delivery Ltd | Transport & logistics | 2025 |
| Easy Eco Transport Ltd | Transport & logistics | 2024 |
| Easy Extend Limited | Professional & scientific | 2026 |
| Easy Fit Limited | Wholesale & retail | 2022 |
| Easy Go Estate and Property Developers Limited | Real estate | 2025 |
| Easy Port Parking Ltd | Transport & logistics | 2025 |
| Easy Ride Taxis Ltd | Transport & logistics | 2024 |
| Easy Shop (Southampton) Ltd | Wholesale & retail | 2025 |
| Easy Solution Accounting Ltd | Professional & scientific | 2012 |
| Easy Tax & Accounting Ltd | Professional & scientific | 2020 |
| Easy TZ Meals CIC | Hospitality | 2023 |
| Easy Way Solutions Ltd | Manufacturing | 2019 |