| E - Create Limited | Wholesale & retail | 1999 |
| E ARC Investments Limited | Financial services | 2017 |
| E Art 4 U Limited | Professional & scientific | 2010 |
| E D Energy Solutions Limited | Construction | 2023 |
| E D Leisure Ltd | Hospitality | 2009 |
| E Green Properties & Building Ltd | Construction | 2025 |
| E Harrison Holdings Limited | Financial services | 2025 |
| E Harvey Management Limited | Administrative & support | 2021 |
| E J Waddington Architecture Limited | Professional & scientific | 2025 |
| E J Waddington Limited | Professional & scientific | 2020 |
| E K C Karate Limited | Arts & recreation | 2014 |
| E K Haulage Ltd | Transport & logistics | 2025 |
| E L O Plumbing Ltd | Construction | 2023 |
| E N Logistics Limited | Transport & logistics | 2021 |
| E N Taylor Ltd | Manufacturing | 2025 |
| E Solutions Sheffield Ltd | Agriculture & fishing | 2016 |
| E Storey Ltd | Wholesale & retail | 2025 |
| E&A Family Property Co. Limited | Real estate | 2025 |
| E&Co Ltd. | Manufacturing | 2015 |
| E&E Carpets Limited | Wholesale & retail | 2012 |
| E&E Connections Limited | Professional & scientific | 2024 |
| E&E Mathew Limited | Real estate | 2021 |
| E&E Motorsport Movements Ltd | Transport & logistics | 2019 |
| E&L Accounting Ltd | Professional & scientific | 2024 |
| E&M Mosley Ltd | Hospitality | 2023 |
| E&O Surgery Ltd | Health & social care | 2021 |
| E-Accountancy Services Limited | Professional & scientific | 2003 |
| E-Bizarre Ltd | Wholesale & retail | 2014 |
| E-Cigsuk Ltd | Wholesale & retail | 2013 |
| E-D-E Consulting Ltd | Professional & scientific | 2018 |
| E-GENER8 Ltd | Construction | 2020 |
| E-Iph Limited | Information & communication | 2016 |
| E-Miza for Tourism Services Ltd | Administrative & support | 2024 |
| E-People Personnel Holdings Limited | Professional & scientific | 2017 |
| E-People Personnel Limited | Professional & scientific | 2010 |
| E-Qualitas Professional Services Limited | Education | 2005 |
| E-Quitz Ltd | Wholesale & retail | 2019 |
| E-Systems Software Limited | Information & communication | 2017 |
| E-Vid Limited | Administrative & support | 2018 |
| E. Pawson & SON Limited | Information & communication | 1974 |
| E. W. Marchpane Limited | Wholesale & retail | 1989 |
| E. Walker (Decorators) Limited | Construction | 1999 |
| E.A. Moore Builders Limited | Construction | 1966 |
| E.B.Broadbent Limited | Transport & logistics | 1960 |
| E.Barker & Son(Hillsboro')Limited | Wholesale & retail | 1931 |
| E.C Quality Systems Consulting Ltd | Information & communication | 2021 |
| E.D.E.N. Film Productions Limited | Information & communication | 2013 |
| E.Desserts Ltd | Hospitality | 2026 |
| E.Dube Limited | Construction | 2016 |
| E.E. Ingleton Engineering Limited | Manufacturing | 1976 |
| E.H Property Limited | Real estate | 2018 |
| E.H.Middleton & Son(Decorators)Limited | Construction | 1950 |
| E.L.Jones Enterprise Limited | Information & communication | 2022 |
| E.Leather & Sons Limited | Real estate | 1959 |
| E.N.A Service Station Ltd | Wholesale & retail | 2017 |
| E.R. Varney (Tools) Limited | Professional & scientific | 1977 |
| E.R.Epic Ltd | Health & social care | 2023 |
| E.W.J. Limited | Real estate | 2015 |
| E1 Couriers Limited | Transport & logistics | 2015 |
| E35 Limited | Real estate | 2018 |
| E35 Properties Limited | Construction | 2024 |
| E4 (Environmental) Limited | Construction | 2006 |
| E46 Tuning Ltd | Wholesale & retail | 2023 |
| E4N Neurodiagnostics Ltd | Health & social care | 2022 |
| EA 7858 Ltd | Other services | 2023 |
| EA Cafe S3 Limited | Hospitality | 2024 |
| EAB Gunduz Ltd | Wholesale & retail | 2025 |
| Eadon Consulting Limited | Professional & scientific | 2008 |
| Eadon Holding Limited | Financial services | 2019 |
| Eadon Lockwood & Riddle ( Lettings ) Limited | Real estate | 2021 |
| Eadon Lockwood & Riddle Limited | Real estate | 2006 |
| Eadon Lockwood and Riddle Holdings Ltd | Financial services | 2025 |
| Eager Beever Dog Training Ltd | Education | 2022 |
| Eagers Properties Ltd | Real estate | 2025 |
| Eagle Decor Ltd | Construction | 2021 |
| Eagle Estate Agents Limited | Administrative & support | 2015 |
| Eagle Eyecare Limited | Health & social care | 2015 |
| Eagle Platforms & Training Ltd | Construction | 2024 |
| Eagle S9 Limited | Households | 2026 |
| Eagles on Wings Ltd | Hospitality | 2014 |
| EAH Services Ltd | Health & social care | 2019 |
| EAI Management Ltd. | Administrative & support | 2016 |
| EAR Again Limited | Health & social care | 2022 |
| EAR Care Yorkshire Limited | Health & social care | 2019 |
| Earl Marshal News Ltd | Wholesale & retail | 2026 |
| Earl Marshall Guest House Ltd | Hospitality | 2010 |
| Earl ST Stores Ltd | Wholesale & retail | 2025 |
| Earlcross Limited | Construction | 2024 |
| Earledge Developments Limited | Construction | 1993 |
| Earls Yard Limited | Hospitality | 2026 |
| Early Bar Ltd | Hospitality | 2019 |
| Early Life Enterprise CIC | Education | 2023 |
| Early Mornings Studio Ltd | Wholesale & retail | 2025 |
| Early Shine Limited | Information & communication | 2024 |
| Early Years Care and Education Ltd | Health & social care | 2017 |
| Early Years People Ltd | Administrative & support | 2025 |
| Earnshaw Art Ltd | Administrative & support | 2003 |
| Ears to Hear Ministries Limited | Other services | 2017 |
| Earth Circle Organics Ltd | Wholesale & retail | 2025 |
| Earth Environ Logistics Limited | Households | 2025 |