| Studio Jamb Ltd | Professional & scientific | 2019 |
| Studio Kato Ltd | Professional & scientific | 2016 |
| Studio Pedretti Ltd | Wholesale & retail | 2017 |
| Studio Polpo Limited | Professional & scientific | 2008 |
| Studio Symbol Limited | Professional & scientific | 2021 |
| Studio Talkes Limited | Wholesale & retail | 2025 |
| Studio Tekne Ltd | Information & communication | 2020 |
| Studio TOD Limited | Arts & recreation | 2007 |
| Studio Twenty One Ltd | Other services | 2019 |
| Studio White Haus Ltd | Professional & scientific | 2023 |
| Studio With JAM Ltd | Professional & scientific | 2025 |
| Studio Zuke Ltd | Information & communication | 2019 |
| STUDIO13MEISNER CIC | Arts & recreation | 2019 |
| Studiodana Ltd | Information & communication | 2025 |
| STUDIOH78 Ltd | Professional & scientific | 2023 |
| Studiomisc Ltd | Information & communication | 2025 |
| Study Pros Consultants Ltd | Education | 2025 |
| Stuff the Office Ltd | Wholesale & retail | 2019 |
| Stumperlowe Estates Limited | Households | 2020 |
| Stumperlowe Mansions (Services) Limited | Households | 1971 |
| Style Decor UK Ltd | Construction | 2008 |
| Style Haven Hub Ltd | Manufacturing | 2026 |
| Style Studio Limited | Wholesale & retail | 2026 |
| Style'D UP Ltd | Other services | 2024 |
| Stylebar Limited | Other services | 2014 |
| Styled by AMY Nicholson Ltd | Other services | 2022 |
| Styleicon Salons Limited | Other services | 2019 |
| Stylelimb Limited | Manufacturing | 2026 |
| Stylish Barber S12 Limited | Other services | 2026 |
| Stylish Collective Ltd | Wholesale & retail | 2021 |
| Stylist Barber S6 Ltd | Other services | 2023 |
| Styr Bistro Limited | Hospitality | 2023 |
| Styr Events Limited | Hospitality | 2024 |
| Styra Ltd | Wholesale & retail | 2015 |
| SU Fleet Ltd | Transport & logistics | 2025 |
| Suatism Limited | Information & communication | 2021 |
| SUB Zero Holdings Limited | Energy | 2019 |
| Sub-Urban Plus Developments Limited | Professional & scientific | 1999 |
| SUB85 Limited | Information & communication | 2013 |
| Subbies United Ltd | Wholesale & retail | 2025 |
| Subbit Structural Detailing Limited | Professional & scientific | 1999 |
| Subcontract Joinery Services Ltd | Construction | 2014 |
| Subnet Calc Limited | Information & communication | 2026 |
| Subscan UDS Limited | Professional & scientific | 2012 |
| Subscription Master Limited | Information & communication | 2014 |
| Subsidium Tech Ltd | Information & communication | 2022 |
| Subway S10 Ltd | Hospitality | 2021 |
| Success in Academia Ltd | Professional & scientific | 2021 |
| Successnuga Services Ltd | Health & social care | 2019 |
| Such & Such Design Limited | Professional & scientific | 2012 |
| Sudanese Community Association in Sheffield C.I.C. | Other services | 2022 |
| Sudart Events Services Ltd | Wholesale & retail | 2020 |
| Sudbrook Engineering Limited | Professional & scientific | 2024 |
| Sude Cleaning Service Ltd | Administrative & support | 2026 |
| SUE Atkinson Aesthetics & Wellness Clinic Ltd | Other services | 2021 |
| SUE Frost Consulting Ltd | Professional & scientific | 2018 |
| SUE Lenkowski Limited | Other services | 2009 |
| SUE Ross Recruitment Limited | Administrative & support | 1999 |
| SUE Rossi Ltd | Wholesale & retail | 2018 |
| Suede Advisory Limited | Professional & scientific | 2023 |
| Suel Limited | Professional & scientific | 1984 |
| Sufc Hotel Ltd | Hospitality | 2020 |
| Sufc Women Limited | Arts & recreation | 2016 |
| Suffiarn ALI Ltd | Transport & logistics | 2026 |
| Suffolks Surveyors Limited | Real estate | 2022 |
| Sufi Heart of Construction Ltd | Construction | 2024 |
| Suga Club Ltd | Hospitality | 2026 |
| Sugar Buzz Limited | Hospitality | 2020 |
| Sugar Plum Cleaning Services Ltd | Administrative & support | 2021 |
| Sugar Rush Desserts Limited | Hospitality | 2021 |
| Sugar Rush Sprinkle Ltd | Wholesale & retail | 2025 |
| Sugar Xpress Ltd | Hospitality | 2019 |
| Sugeon Ltd | Wholesale & retail | 2025 |
| Sugworth Estates Limited | Real estate | 2021 |
| Sujitech Limited | Professional & scientific | 2020 |
| Sukaan Limited | Households | 2017 |
| Sukoon Care & Support Ltd | Administrative & support | 2026 |
| Suli Tyre Services Ltd | Wholesale & retail | 2024 |
| Sultan OUD and Perfumes Ltd | Manufacturing | 2026 |
| Sultan Property Services Limited | Real estate | 2022 |
| Sumbdex Limited | Health & social care | 2015 |
| Sumedha Pharma Limited | Health & social care | 2019 |
| Sumera Saleem Limited | Wholesale & retail | 2022 |
| Summer Catering Ltd | Manufacturing | 2024 |
| Summer Developments Ltd | Real estate | 2025 |
| Summer House Project Management Limited | Construction | 2023 |
| Summer Retail Limited | Transport & logistics | 2001 |
| Summercare & Recruitment Services Limited | Administrative & support | 2023 |
| Summerfield Street Management Company Limited | Households | 2007 |
| Summerhouse Bouquets Ltd | Wholesale & retail | 2025 |
| Summerhouse Motor Company Ltd | Wholesale & retail | 2020 |
| Summers Day Camper Components Ltd | Manufacturing | 2022 |
| Summers Day Campers North Ltd | Manufacturing | 2023 |
| Summers Day Campers South Ltd | Manufacturing | 2023 |
| Summers Family Holdings Limited | Financial services | 2023 |
| Summersday Campers Ltd | Manufacturing | 2020 |
| Summit IT Ltd | Information & communication | 2018 |
| Summit Projects Limited | Professional & scientific | 2008 |
| Summit Systems Outdoor Equipment Ltd | Manufacturing | 2025 |
| Summit Transit Limited | Transport & logistics | 2024 |