| Spirit Beer Management Limited | Professional & scientific | 2024 |
| Spirit LAB Cocktails Ltd | Wholesale & retail | 2021 |
| Spirit of Independence Film Festival Limited | Information & communication | 2018 |
| Spirit of MAN Ltd | Other services | 2020 |
| Spirit of Sheffield Ltd | Manufacturing | 2018 |
| Spirit Personal Training Limited | Arts & recreation | 2006 |
| Spirit Store Ltd | Wholesale & retail | 2020 |
| Spiritshack Ltd | Wholesale & retail | 2021 |
| Spiritualized Limited | Administrative & support | 2003 |
| Spit IT OUT Productions Limited | Information & communication | 2024 |
| Spital Food Ltd | Hospitality | 2023 |
| Spital Minimarket Limited | Wholesale & retail | 2025 |
| Spital Properties Limited | Real estate | 2026 |
| Spital Shawarma Ltd | Hospitality | 2025 |
| Spkp Property Management Limited | Real estate | 2021 |
| Splash Cleaning Business Ltd | Administrative & support | 2026 |
| Splashes Ltd | Administrative & support | 2014 |
| Splendent Ltd | Hospitality | 2017 |
| Splendid Cakes Ltd | Manufacturing | 2024 |
| Splinters Theatre Group | Arts & recreation | 2024 |
| Split Screen Music Ltd | Information & communication | 2025 |
| SPM 1C Limited | Extraterritorial | 2023 |
| SPM 1K Limited | Agriculture & fishing | 2023 |
| SPM Cleaning Company Ltd | Administrative & support | 2024 |
| SPM Legal CIC | Professional & scientific | 2024 |
| Spoil Limited | Wholesale & retail | 2025 |
| Spoilt for Choice ( Catering) Ltd | Hospitality | 2011 |
| Spongl Ltd | Information & communication | 2018 |
| Spoon Graphics Ltd | Information & communication | 2014 |
| Spoonfest Limited | Administrative & support | 2012 |
| Sport Board Games Limited | Wholesale & retail | 2023 |
| Sport Card Collectables (Yorkshire) Limited | Wholesale & retail | 2024 |
| Sport Family Limited | Wholesale & retail | 2011 |
| Sport Shack South Yorkshire Ltd | Hospitality | 2025 |
| Sport Shack SS Limited | Hospitality | 2025 |
| SPORT:80 Limited | Information & communication | 2013 |
| SPORT:80 Services Limited | Information & communication | 2012 |
| Sporting Direct Limited | Arts & recreation | 2019 |
| Sportpromote Limited | Education | 2016 |
| Sports Accessories 1 Limited | Wholesale & retail | 2023 |
| Sports Knees Limited | Health & social care | 2026 |
| Sports Overlays Ltd | Construction | 2019 |
| Sports Streaming UK Ltd | Information & communication | 2016 |
| Sportserve Limited | Administrative & support | 2012 |
| Sportsio Limited | Arts & recreation | 2025 |
| Sportsman Inn Hackenthorpe Ltd | Arts & recreation | 2023 |
| Sportsprep Limited | Arts & recreation | 2015 |
| Spot Commerce Limited | Wholesale & retail | 2025 |
| Spot on Furniture Ltd | Wholesale & retail | 2022 |
| Spot on Pubs Ltd | Hospitality | 2024 |
| Spot on Tyres Ltd | Wholesale & retail | 2025 |
| Spotless Valeting Centre Limited | Wholesale & retail | 2017 |
| Spray Centre (U.K.) Limited | Wholesale & retail | 1995 |
| Spray Foam Equipment UK Ltd | Wholesale & retail | 2019 |
| Spray Nine Europe Limited | Manufacturing | 2000 |
| Spray Solutions S9 Ltd | Wholesale & retail | 2026 |
| Spray-Tec (Sheffield) Limited | Wholesale & retail | 2005 |
| Spraybooth Engineering Limited | Professional & scientific | 2019 |
| Spraytec Engineering Limited | Professional & scientific | 1994 |
| Spriee Ltd | Transport & logistics | 2022 |
| Spring &Sons Ltd | Transport & logistics | 2022 |
| Spring Tank Studios Ltd | Information & communication | 2024 |
| Spring Yard Ltd | Hospitality | 2019 |
| Springbank KOI Ltd | Wholesale & retail | 2016 |
| Springcoil Limited | Manufacturing | 2005 |
| Springfield Fostering Agency Ltd | Health & social care | 2022 |
| Springfield Grange Limited | Real estate | 2017 |
| Springfield Vale Health Care Limited | Health & social care | 2023 |
| Springpalm Limited | Real estate | 1976 |
| Springs Consulting Limited | Professional & scientific | 2012 |
| Springs Electrical Services Ltd | Construction | 2026 |
| Springvale Fire Safety Consultants Ltd | Public administration | 2025 |
| Springview Ltd | Real estate | 2012 |
| Springwood Aesthetics Ltd | Other services | 2026 |
| Springwood DMC Ltd | Arts & recreation | 2021 |
| Springwood Project Consulting Limited | Information & communication | 2022 |
| Sprite Solutions Ltd | Information & communication | 2015 |
| Sproggies Ltd | Extraterritorial | 2023 |
| Sprout Food Co. Ltd | Hospitality | 2025 |
| Sprowston Freehold Ltd | Real estate | 2016 |
| Spruce Living Limited | Real estate | 2019 |
| SPS Building Maintenance & Improvements Ltd | Construction | 2023 |
| SPS Property Enterprise Ltd | Real estate | 2018 |
| SPS Site Services Ltd | Construction | 2015 |
| SPS Tech Consulting Limited | Information & communication | 2025 |
| SPT Fabs Ltd | Professional & scientific | 2025 |
| Spud Nation Ltd | Hospitality | 2025 |
| Spud Scene Limited | Hospitality | 2025 |
| Spunkydj Entertainment Ltd | Arts & recreation | 2025 |
| Spyglass Media Limited | Information & communication | 2011 |
| SQ Collective Ltd | Health & social care | 2026 |
| SQ Content Ltd | Administrative & support | 2025 |
| SQ Publishing Ltd | Information & communication | 2019 |
| SQA Professionals Ltd | Professional & scientific | 2026 |
| Sqas Ltd | Professional & scientific | 2025 |
| SQR Accountancy Ltd | Professional & scientific | 2019 |
| SQR Lifestyle Ltd | Wholesale & retail | 2019 |
| Square Contracts Limited | Administrative & support | 2021 |
| Square Dog Ltd | Real estate | 2011 |
| Square Edge Investments Limited | Financial services | 2011 |