| Sophia-Vaduva Medical Limited | Health & social care | 2021 |
| Sophie Kirkton Limited | Professional & scientific | 2023 |
| Sophie Loudoun-Shand Ltd | Professional & scientific | 2023 |
| Sophie Morrell Limited | Information & communication | 1999 |
| Sophie's Home Supplies Ltd | Wholesale & retail | 2021 |
| Sophienna Holdings Ltd | Financial services | 2026 |
| Sophies Travel Escapes Ltd | Administrative & support | 2024 |
| Sophisticated PR Ltd | Professional & scientific | 2024 |
| Sopore Foods Limited | Hospitality | 2024 |
| Sorb Holdings Limited | Financial services | 2020 |
| Sorelle UK Brand Limited | Wholesale & retail | 2016 |
| Sorrel Consturctions Ltd | Construction | 2025 |
| Sort My Bill Limited | Information & communication | 2025 |
| Sort Of...Films Limited | Information & communication | 2003 |
| Sorted + Supported Ltd | Professional & scientific | 2026 |
| Sorted 4 Safety Limited | Wholesale & retail | 2006 |
| Sorted Carman Ltd | Extraterritorial | 2025 |
| Soryaz Trending Ltd | Wholesale & retail | 2025 |
| SOS for Sudan CIC | Professional & scientific | 2024 |
| Soso Trans Ltd | Transport & logistics | 2019 |
| Sota Interiors Limited | Manufacturing | 2022 |
| SOTERIA360 Limited | Information & communication | 2023 |
| Soth Limited | Health & social care | 2019 |
| Sothall Green Management Company Limited | Hospitality | 2003 |
| Sothall Roofing Partnership | | 2005 |
| Soto Ltd | Real estate | 2020 |
| Soufyan Limited | Transport & logistics | 2025 |
| Soukify Ltd | Wholesale & retail | 2025 |
| Soul Bowl Ltd | Hospitality | 2026 |
| Soul Fire Hot Yoga Ltd | Other services | 2023 |
| Soul Fire Studio Spaces Ltd | Real estate | 2023 |
| Soul Sun Coaching Ltd | Health & social care | 2024 |
| Soulfood Spices Limited | Hospitality | 2023 |
| Soulful Bars and Restaurants Limited | Hospitality | 2015 |
| Soulful Roots Ltd | Wholesale & retail | 2025 |
| Soulpunk Ltd | Arts & recreation | 2016 |
| Soulramen Japan Ltd | Hospitality | 2026 |
| Soulyaki Ltd | Hospitality | 2025 |
| Sound Artists Limited | Administrative & support | 2023 |
| Sound Cafe Fest Ltd | Health & social care | 2024 |
| Sound Ideas Media Limited | Information & communication | 2003 |
| Sound of Islay Holdings Limited | Real estate | 2025 |
| Soundhouse Media Limited | Information & communication | 2000 |
| Soundpost Community Network | Arts & recreation | 2016 |
| Soundpost Live Ltd | Arts & recreation | 2016 |
| Soundstreaming Ltd | Arts & recreation | 2023 |
| Soup N Bread Ltd | Transport & logistics | 2026 |
| Source My Mortgage Ltd | Financial services | 2020 |
| Source Pro Limited | Information & communication | 2023 |
| Source Sounds Ltd | Wholesale & retail | 2010 |
| Sourced Software Limited | Information & communication | 2019 |
| Sourcevox Ltd | Information & communication | 2009 |
| Sourcify Trading Limited | Wholesale & retail | 2025 |
| Sourcing Worldwide Ltd | Extraterritorial | 2008 |
| Sout Coffee UK Ltd | Hospitality | 2025 |
| South & West Yorkshire IFA Limited | Financial services | 2015 |
| South American Energy LLP | | 2020 |
| South Anglia Limited | Manufacturing | 2015 |
| South Coast Building & Maintenance Ltd | Construction | 2018 |
| South Coast Roofing Supplies Limited | Extraterritorial | 1984 |
| South Indian Crookes Limited | Hospitality | 2025 |
| South LEA Technology Ltd | Professional & scientific | 1997 |
| South Milton Management Limited | Real estate | 2003 |
| South Sheffield Community Initiatives Ltd | Arts & recreation | 2008 |
| South Stone Driveways Limited | Administrative & support | 2026 |
| South West Building Solutions Ltd | Construction | 2019 |
| South West Cardiology Limited | Health & social care | 2020 |
| South West Support Limited | Health & social care | 2014 |
| South Yorkshire and Derbyshire Cardiology Clinic Ltd | Health & social care | 2019 |
| South Yorkshire Auctions Ltd | Extraterritorial | 2013 |
| South Yorkshire Auto Diagnostics Limited | Wholesale & retail | 2005 |
| South Yorkshire Bathroom Design Limited | Construction | 2010 |
| South Yorkshire Bike Manufacturing Ltd | Manufacturing | 2024 |
| South Yorkshire Builders Ltd | Construction | 2024 |
| South Yorkshire Business Advisors Limited | Professional & scientific | 2021 |
| South Yorkshire Car Parts Limited | Wholesale & retail | 2018 |
| South Yorkshire Catering Limited | Hospitality | 2024 |
| South Yorkshire Chess & Education Centre Community Interest Company | Education | 2023 |
| South Yorkshire Community Foundation Limited | Professional & scientific | 2011 |
| South Yorkshire Drainage and Cleaning Specialists Ltd | Administrative & support | 2018 |
| South Yorkshire Eating Disorders Association | Health & social care | 2006 |
| South Yorkshire Fabrications Limited | Manufacturing | 2025 |
| South Yorkshire Future Trams Ltd | Transport & logistics | 2023 |
| South Yorkshire Heart Centre Ltd | Health & social care | 2024 |
| South Yorkshire Heritage Buses Limited | Transport & logistics | 2008 |
| South Yorkshire HIP and Knee Clinic Limited | Health & social care | 2025 |
| South Yorkshire Investment Capital Fund | | 2001 |
| South Yorkshire Investment Development Fund | | 2001 |
| South Yorkshire Investments Limited | Administrative & support | 2005 |
| South Yorkshire Joinery Limited | Manufacturing | 1994 |
| South Yorkshire Management Limited | Wholesale & retail | 2015 |
| South Yorkshire Mechanical Services Limited | Construction | 2019 |
| South Yorkshire Mixed Martial Arts Academy CIC | Arts & recreation | 2020 |
| South Yorkshire Orthopaedic Services Limited | Health & social care | 2008 |
| South Yorkshire Pattern Co. Limited | Manufacturing | 2001 |
| South Yorkshire Police Sports and Social Club Limited | Arts & recreation | 2001 |
| South Yorkshire Printers Limited | Manufacturing | 1982 |
| South Yorkshire Properties Services Ltd | Construction | 2017 |
| South Yorkshire Property Investment Company Limited | Financial services | 2012 |
| South Yorkshire Property Ltd | Construction | 2020 |