| 1069 Taxis Ltd. | Professional & scientific | 2014 |
| 21 23 Ltd | Real estate | 2026 |
| 4T4 Ltd | Real estate | 2024 |
| A Bruce Hastie Electrical Contractor Ltd | Construction | 2020 |
| A. Elliot (Fine Fabrics) Limited | Manufacturing | 1965 |
| A.B Northampton Limited | Professional & scientific | 2013 |
| A7 Waverley Auctions Limited | Wholesale & retail | 2026 |
| Abbey Lifestyle Trading Limited | Other services | 2025 |
| Abbey Machinery Hire Limited | Other services | 2025 |
| Abundita Ltd | Financial services | 2023 |
| Acton Lang Ltd | Real estate | 2021 |
| Agrex Distribution Limited | Financial services | 2026 |
| Aikwood Tower Ltd | Hospitality | 2010 |
| Akam Care Limited | Health & social care | 2010 |
| Alison Gray Accountancy Limited | Professional & scientific | 2010 |
| ALL Seasons Philipburn Ltd | Hospitality | 2023 |
| Alnawajed Ltd | Health & social care | 2026 |
| Altrieve Accountancy Limited | Professional & scientific | 2013 |
| Altrieve Taxation Limited | Professional & scientific | 2014 |
| Amaklogistics Ltd | Transport & logistics | 2025 |
| AMS Health Ltd | Health & social care | 2022 |
| Amtul Rauf Retail Limited | Wholesale & retail | 2025 |
| Andersen Legal Consulting Limited | Professional & scientific | 2019 |
| Andrew Hood Joinery Ltd | Construction | 2020 |
| Annelshope Holdings Ltd | Financial services | 2024 |
| Aqtir Limited | Health & social care | 2015 |
| Aquaaura Direct Limited | Wholesale & retail | 2025 |
| ARN Healthcare Ltd | Health & social care | 2025 |
| AS48 Ltd | Wholesale & retail | 2018 |
| Ashcraig Farmers Limited | Agriculture & fishing | 2004 |
| Ashkirk Land Ltd | Real estate | 2019 |
| Ashkirktown Farmers | | 1996 |
| Asset Funding Ltd | Information & communication | 2017 |
| Autistic Therapist Directory CIC | Health & social care | 2026 |
| AV Safety Consultants Limited | Professional & scientific | 2016 |
| Avani Sethi Ltd | Health & social care | 2024 |
| B & H Property (Scotland) Ltd | Real estate | 2010 |
| B Q Farms Limited | Agriculture & fishing | 1977 |
| BAD Buunda Ltd | Health & social care | 2021 |
| Bajr Ltd | Professional & scientific | 2020 |
| Balance Point Ltd | Professional & scientific | 2024 |
| Barakah Solutions Limited | Administrative & support | 2020 |
| Bas:X Art Community Interest Company | Arts & recreation | 2020 |
| BCT Trustees Limited | Professional & scientific | 2021 |
| BDS (Scotland) Ltd | Real estate | 2024 |
| Beaton Forestry Limited | Agriculture & fishing | 2008 |
| Bellendean Limited | Financial services | 1962 |
| Best Fishing Ltd | Arts & recreation | 2017 |
| Best Intent Marquees Limited | Administrative & support | 2015 |
| Better Berwick Ltd | Professional & scientific | 2021 |
| Billet Brown Ltd | Manufacturing | 2022 |
| Black Silver Ltd | Information & communication | 2016 |
| Blether Media Ltd | Information & communication | 2024 |
| Blue Seafront (T/A Hutchesons of Portsoy) Ltd | Wholesale & retail | 2024 |
| Boardroom Punks Ltd | Information & communication | 2022 |
| Bonington Green Ltd | Information & communication | 2018 |
| Bonington PLS Ltd | Professional & scientific | 2019 |
| Bordent Limited | Health & social care | 2015 |
| Border Archery Limited | Manufacturing | 1976 |
| Border Bathrooms Ltd | Construction | 2026 |
| Border Hire Limited | Administrative & support | 2024 |
| Border Plant & Hydraulics Ltd | Manufacturing | 2026 |
| Border Safeguard Limited | Administrative & support | 1979 |
| Borders Bean Counters Ltd | Professional & scientific | 2023 |
| Borders Business Solutions Limited | Professional & scientific | 2013 |
| Borders Direct Payment Agency | Administrative & support | 2008 |
| Borders Refrigeration and Air Conditioning Ltd | Construction | 2022 |
| Borders Safety Supplies Limited | Administrative & support | 2008 |
| Bowhill Farming Limited | Agriculture & fishing | 2017 |
| BPV (Scotland) Ltd | Real estate | 2025 |
| Brady Associates Ltd. | Professional & scientific | 2005 |
| Bridge Scottish Borders CIC | Administrative & support | 2025 |
| Bridgelands Enterprises Limited | Financial services | 2025 |
| Bridgelands Farm Limited | Agriculture & fishing | 2024 |
| Bright Arts CIC | Arts & recreation | 2024 |
| Brighton Road Dental Care Limited | Health & social care | 2025 |
| Broadmeadows Associates Limited | Hospitality | 2012 |
| Broadmeadows Estates Limited | Real estate | 2020 |
| Broadmeadows Financial Planning Ltd | Financial services | 2020 |
| Broomknoll Ltd | Health & social care | 2022 |
| Brown & Brown Ltd | Health & social care | 2019 |
| Brown & Muir Limited | Construction | 2011 |
| Buccleuch Estates, Ltd. (the) | Financial services | 1923 |
| Buccleuch Sporting Limited | Arts & recreation | 2024 |
| Buccleuch Woodlands Enterprises Limited | Agriculture & fishing | 2005 |
| Buccleuch Woodlands Limited | Agriculture & fishing | 1982 |
| Burnfoot Farmers | | 1996 |
| Burnside Dental Limited | Health & social care | 2025 |
| Byspel Ltd | Professional & scientific | 2018 |
| C Thomson Builders Ltd | Construction | 2026 |
| C&C Mcbride Ltd | Health & social care | 2024 |
| Celtman Ltd | Arts & recreation | 2012 |
| Charbertton Ltd | Real estate | 2026 |
| Charles Brodie Ltd | Construction | 2015 |
| Cherrypiq Ltd | Information & communication | 2022 |
| Chlodan Consultancy Ltd | Professional & scientific | 2018 |
| Circet Home Holdings Limited | Professional & scientific | 2011 |
| Circet Home Limited | Information & communication | 2007 |
| Citoa Ltd | Wholesale & retail | 2021 |
| Clark Elliott Property Developments Limited | Construction | 2013 |