| Clarkson Motors Limited | Wholesale & retail | 2002 |
| Claymore Events Limited | Hospitality | 2020 |
| CMC Dental Limited | Health & social care | 2022 |
| County Retreat Limited | Hospitality | 2025 |
| CPS Robson Property Ltd | Households | 2021 |
| Craig Thomson Engineering Limited | Manufacturing | 2024 |
| Creag Erica Ltd | Health & social care | 2025 |
| Cross Keys Inn (Ettrickbridge) Limited | Hospitality | 2022 |
| Csmej Limited | Health & social care | 2020 |
| Curam-Slainte (Scotland) Limited | Health & social care | 2016 |
| Dalmeny Property Company Limited | Real estate | 2007 |
| Danada Investments Ltd | Health & social care | 2025 |
| Datamars Agri UK Ltd | Manufacturing | 2004 |
| DEE GEE Scanning Limited | Professional & scientific | 2013 |
| Dencare+ Ltd | Information & communication | 2025 |
| Dental & Aesthetics Ltd | Health & social care | 2024 |
| Dental Accountants Scotland Ltd | Professional & scientific | 2016 |
| Dental Practice Owners Scotland Ltd | Health & social care | 2020 |
| Dentologyc Ltd | Health & social care | 2026 |
| Diamond Dental Care (Clydebank) Limited | Health & social care | 2025 |
| Douglas and Hardie Joinery Limited | Construction | 2024 |
| DR HI Ltd | Health & social care | 2022 |
| DR L Harris Radiology Ltd | Health & social care | 2024 |
| Drivemedia Limited | Manufacturing | 2003 |
| Drummond Melville (UK) Ltd | Information & communication | 2010 |
| Dsys Prop Limited | Real estate | 2022 |
| Dunsdale Coachworks Ltd | Wholesale & retail | 2014 |
| Dunsdale Properties Limited | Real estate | 2013 |
| Dunsdalehaugh Holdings Ltd. | Real estate | 2007 |
| Durn Black Ltd | Information & communication | 2024 |
| Durty Brewing Ltd | Manufacturing | 2020 |
| Durty Events Limited | Arts & recreation | 2011 |
| E Macd Plumbing & Heating Contractors Ltd | Construction | 2021 |
| Eadie Bros & Co Ltd | Manufacturing | 2015 |
| Earning Smiles Ltd | Health & social care | 2020 |
| Ecohus Ltd | Professional & scientific | 2014 |
| Eildon Enterprise Limited | Real estate | 2004 |
| EJH Translations Ltd | Professional & scientific | 2014 |
| Elliot Henderson & SON Ltd | Construction | 2015 |
| Elliot Henderson Holdings Limited | Agriculture & fishing | 1997 |
| Elliot Henderson Limited | Agriculture & fishing | 2000 |
| Emond Engineering Ltd | Professional & scientific | 2025 |
| Emzar Limited | Hospitality | 2025 |
| Ergo Energy Ltd | Energy | 2014 |
| Ettrick and Yarrow Community Development Company | Public administration | 2013 |
| Ettrick Computer Services Limited | Information & communication | 1990 |
| Ettrick Yarn Dyers Limited | Manufacturing | 2000 |
| Eycdc Homes Ltd | Households | 2022 |
| Fair Networks Limited | Information & communication | 2022 |
| Ferg Mcculloch Consulting Ltd | Professional & scientific | 2018 |
| Ferry Smile Studios Ltd | Health & social care | 2023 |
| Fillin Station Limited | Hospitality | 2016 |
| Fineunit Limited | Professional & scientific | 1990 |
| Fitzpatrick Dental Ltd | Health & social care | 2022 |
| Fleece Kitchen & Bar Ltd | Hospitality | 2024 |
| Formatank Limited | Manufacturing | 2012 |
| Fortunas Property Limited | Real estate | 2024 |
| Four Seasons Contracting Limited | Agriculture & fishing | 2014 |
| Four Seasons Forestry Services Limited | Agriculture & fishing | 2015 |
| Freelance Business Services Limited | Professional & scientific | 2019 |
| Friends of the George Limited | Arts & recreation | 2023 |
| Funding Plus Services Ltd | Information & communication | 2016 |
| Fusion Investments Group Ltd | Financial services | 2023 |
| G Blair Decor Ltd | Construction | 2013 |
| Game and Country Feeds Limited | Wholesale & retail | 2022 |
| Game and Country Holdings Limited | Financial services | 2026 |
| Game and Country Limited | Wholesale & retail | 2009 |
| Gateway Transport Scotland Limited | Transport & logistics | 2012 |
| Gavin Henderson Ltd | Real estate | 2019 |
| Gdmscot Ltd | Health & social care | 2022 |
| Geologis Ltd. | Professional & scientific | 2010 |
| Gillgrass Ltd | Health & social care | 2024 |
| Gilmour Pike Chapman Limited | Professional & scientific | 2026 |
| Gledknowe Farming Company | | 2000 |
| Glen Cafe at ST Mary's Loch Ltd | Hospitality | 2026 |
| GM Decoration Ltd | Construction | 2021 |
| Granton Assets Limited | Financial services | 1932 |
| GSP Digital Solutions Ltd | Information & communication | 2022 |
| GSP Solutions Holdings Limited | Financial services | 2025 |
| Guardian of Scotland (Selkirk) Ltd. | Manufacturing | 2012 |
| Guild (Edinburgh) Limited | Professional & scientific | 2017 |
| Haining Garden Accounting Limited | Professional & scientific | 2025 |
| Haining Garden Cottage Ltd | Agriculture & fishing | 2023 |
| Happy Hounds Borders Limited | Administrative & support | 2023 |
| Hartwood Logging Ltd | Agriculture & fishing | 2012 |
| HAS Healthcare Limited | Health & social care | 2025 |
| Haus of Harmony Ltd | Arts & recreation | 2025 |
| Hawthorn Sporting LLP | | 2015 |
| Heard Hamilton Financial Planning Ltd | Financial services | 2014 |
| Hearsharp Limited | Wholesale & retail | 2020 |
| Henderson and Mcintosh | | 1996 |
| Henderson Structural Design Ltd | Professional & scientific | 2022 |
| Heroes of Gamenight CIC | Other services | 2023 |
| Highland Stores Ltd | Wholesale & retail | 2026 |
| Hobbs Cornerstone Ltd | Real estate | 2024 |
| Hobbs Imperial Limited | Real estate | 2025 |
| Hobbs International Ltd | Financial services | 2023 |
| Hobbs Keystone Ltd | Real estate | 2024 |
| Holmes Dental Solutions Limited | Health & social care | 2026 |
| Imladris Dental Limited | Health & social care | 2026 |