| Rufus & Belle Ltd | Administrative & support | 2022 |
| Ryde Chiropractic Limited | Health & social care | 2018 |
| Rylstone Tea Gardens Limited | Hospitality | 2015 |
| S W Heating and Plumbing Limited | Energy | 2003 |
| Safe Harbour 05800420 Limited | Administrative & support | 2006 |
| Saffa Properties Limited | Real estate | 2019 |
| Saffrons Limited | Hospitality | 2001 |
| Sage Bakery and Coffee Ltd | Wholesale & retail | 2025 |
| Sandham Hall Limited | Hospitality | 2026 |
| Sandown and Lake Football Club Community Interest Company | Arts & recreation | 2021 |
| Sandown Beach HUT Owners Limited | Professional & scientific | 1998 |
| Sandown Bowls & Sports Club (1926) Limited | Arts & recreation | 2014 |
| Sandown Emporium Limited | Wholesale & retail | 2024 |
| Sandown Hand Car Wash Ltd | Other services | 2019 |
| Sandown Hangars Ltd | Manufacturing | 2017 |
| Sandown Jewellers Limited | Wholesale & retail | 2023 |
| Sandown Masonic Centre Ltd | Other services | 2021 |
| Sandown News Ltd | Wholesale & retail | 2026 |
| Sandown Nursing Home LLP | | 2023 |
| Sandringham Hotels (Isle of Wight) Limited | Hospitality | 1977 |
| Sandy Bay Apartments Limited | Real estate | 2016 |
| Sapphire Project Management Limited | Information & communication | 2005 |
| Sarah B Yoga Ltd | Education | 2021 |
| Sarvanga Yoga Studio Ltd | Education | 2023 |
| Scores Reformed Limited | Manufacturing | 2014 |
| Scott Adds Carpentry Ltd | Construction | 2022 |
| SDW Building Services Ltd | Construction | 2021 |
| Seahaven MAN Co Ltd | Households | 2024 |
| Seashoresparky Ltd | Construction | 2025 |
| Seashoreyogaphysio Ltd | Health & social care | 2025 |
| Seaview Deli Ltd | Hospitality | 2019 |
| Servicepoint Ltd | Information & communication | 2007 |
| SF Research Herts Limited | Professional & scientific | 2020 |
| Shakti Yogis Ltd | Education | 2019 |
| Share,Learn,Grow Ltd | Health & social care | 2026 |
| Shiny Holdings Ltd | Financial services | 2019 |
| Shiny Homes Ltd | Construction | 2013 |
| Shiny PAY Ltd | Information & communication | 2025 |
| Shirescope Limited | Agriculture & fishing | 2026 |
| Similate Limited | Information & communication | 2008 |
| SIX40 Kitchens IOW Ltd | Hospitality | 2025 |
| SJT Construction (IW) Ltd. | Water & waste | 2012 |
| Slecktlive Property Management Limited | Other services | 2018 |
| SLR Maritime Limited | Professional & scientific | 2025 |
| Smart Island Developments Ltd | Construction | 2014 |
| Solent Beds & Sofas Limited | Wholesale & retail | 2008 |
| Solent Developments IOW Limited | Construction | 2026 |
| Solent Hot Tubs Limited | Construction | 2014 |
| Solent Pools and Hot Tubs Limited | Construction | 2019 |
| Solent Youth Services Limited | Health & social care | 2017 |
| Soma Space Ltd | Arts & recreation | 2023 |
| Soma Yoga Ltd | Education | 2023 |
| Somatic Therapeutic Yoga Training Ltd | Education | 2023 |
| Somerton Fleet Limited | Wholesale & retail | 2003 |
| Sonder Movement Ltd | Arts & recreation | 2024 |
| South Coast Flagpole Installation Ltd | Construction | 2019 |
| Specialist OIL Services Limited | Other services | 2018 |
| Sphere VHD Ltd | Hospitality | 2023 |
| Spotless & Sparkling Cleaning Services Ltd | Administrative & support | 2025 |
| ST Claire Resident Ltd | Households | 2022 |
| ST Lawrence Landscapes Ltd | Administrative & support | 2026 |
| Stainless Software Limited | Professional & scientific | 1994 |
| Starr Studios Ltd | Education | 2025 |
| Stassi-Dent Limited | Health & social care | 2015 |
| Steinmetz Pilates and Wellness Ltd | Arts & recreation | 2020 |
| Steve Hunt Mortgage Solutions Ltd | Financial services | 2025 |
| Stoneleigh Freehold Limited | Households | 2012 |
| Stour Design Ltd | Professional & scientific | 2021 |
| Support to Strive Ltd | Health & social care | 2025 |
| Surejust Fittings Limited | Manufacturing | 2012 |
| Surejust Limited | Administrative & support | 1988 |
| Surejust Management Ltd | Financial services | 2024 |
| Susa Engineering & Consultancy Limited | Mining & quarrying | 2013 |
| Synergy Properties IOW Limited | Real estate | 2017 |
| Sysworkah Ltd | Information & communication | 2023 |
| T T Technical Services (IW) Limited | Professional & scientific | 1996 |
| T.R Kitchen Supplies Limited | Wholesale & retail | 2025 |
| Target Technologies Ltd | Arts & recreation | 2009 |
| Target Trading IW Ltd | Construction | 2020 |
| Tayla Ltd | Professional & scientific | 2024 |
| Teehutgolf Limited | Arts & recreation | 2024 |
| Telestonet Ltd | Information & communication | 2019 |
| THE Acorns (Wootton) Limited | Households | 2004 |
| THE Barn Brading Limited | Hospitality | 2023 |
| THE Bugle Inn Brading Ltd | Hospitality | 2024 |
| THE Catertemp Group Ltd | Hospitality | 2022 |
| THE Couple Consultancy Ltd | Education | 2019 |
| THE Fruit Basket (Stubbington) Limited | Wholesale & retail | 2024 |
| THE Granary Management Co Limited | Real estate | 1988 |
| THE Healing Hammock Limited | Health & social care | 2017 |
| THE Holistic Emporium Limited | Wholesale & retail | 2021 |
| THE House of Chilli IOW Ltd | Wholesale & retail | 2025 |
| THE Island Tourism Partnership (IOW) Ltd | Information & communication | 2025 |
| THE Isle of Wight Cheese Company Limited | Manufacturing | 2005 |
| THE Isle of Wight Garlic Festival Limited | Arts & recreation | 2013 |
| THE Marx Brothers Cabin Limited | Information & communication | 2024 |
| THE Media House IOW Limited | Other services | 2022 |
| THE Modern Home Farm Ltd | Agriculture & fishing | 2021 |
| THE Movement Yoga Ltd | Arts & recreation | 2020 |
| THE National POO Museum Ltd | Arts & recreation | 2016 |