| Isle Clean Ltd | Professional & scientific | 2007 |
| Isle of Therapy Ltd | Education | 2016 |
| Isle of Wight Bees Ltd | Manufacturing | 2019 |
| Isle of Wight Chamber of Commerce | Administrative & support | 1973 |
| Isle of Wight Community BUS Partnership Limited | Transport & logistics | 2012 |
| Isle of Wight Experience Limited | Administrative & support | 1997 |
| Isle of Wight Film Fixers C.I.C. | Information & communication | 2021 |
| Isle of Wight Freshwater Angling Company | Arts & recreation | 2014 |
| Isle of Wight Hotels Ltd | Hospitality | 1984 |
| Isle Recycle Limited | Other services | 2020 |
| Isle Store Ltd | Transport & logistics | 2025 |
| Islestart Ltd | Manufacturing | 2021 |
| IW Mixologist Limited | Hospitality | 2023 |
| J Rackstraw Ltd | Manufacturing | 2025 |
| J'S Landscaping & Groundworks Ltd | Administrative & support | 2025 |
| Jaren Bathrooms Ltd | Construction | 2021 |
| Jaren Property Ltd | Real estate | 2019 |
| JHW Composites Ltd | Manufacturing | 2026 |
| JKM Corp Ltd | Information & communication | 2019 |
| JLW Charters Ltd | Arts & recreation | 2025 |
| JM Electrical IW Ltd | Construction | 2015 |
| JML Blades Ltd | Manufacturing | 2023 |
| Jonathan Peat Limited | Professional & scientific | 2021 |
| Joy Yoga Limited | Health & social care | 2015 |
| Julie Crowe Limited | Information & communication | 2025 |
| Julius Robson Ltd | Professional & scientific | 2009 |
| Just for YOU Holiday Homes Ltd | Hospitality | 2020 |
| K L Services (IOW) Limited | Administrative & support | 2011 |
| K&E Marine Services Ltd | Transport & logistics | 2024 |
| Karona Consulting Limited | Information & communication | 2005 |
| Kaypee Design Limited | Professional & scientific | 2006 |
| Keert CIC | Transport & logistics | 2022 |
| Kelly's Way - Eapava Limited | Administrative & support | 2025 |
| Keycom Management Limited | Real estate | 2000 |
| Kfarmer Ltd | Agriculture & fishing | 2009 |
| Kiely Social Care Limited | Health & social care | 2016 |
| Kingsmead Cards Ltd | Manufacturing | 2023 |
| Kingswood Court (IW) Limited | Construction | 2006 |
| Kingswood Court (Sandown Isle of Wight) Limited | Administrative & support | 2008 |
| Knighton Glamping Limited | Hospitality | 2022 |
| KOE Wind Ltd | Construction | 2021 |
| Kreative Studios Ltd | Professional & scientific | 2017 |
| L&R Plumbing Services IW Ltd | Construction | 2019 |
| Lake Autocentre Ltd | Wholesale & retail | 2025 |
| Lake Cleaning & Catering Supplies Limited | Wholesale & retail | 1984 |
| Lake Laundry Services Limited | Other services | 2018 |
| LAKE50 Kebab Ltd | Hospitality | 2026 |
| Lakeside House Management (Isle of Wight) Limited | Real estate | 1974 |
| Landguard Manor Management Limited | Professional & scientific | 2007 |
| Lanyon-Hogg Architects Limited | Professional & scientific | 2003 |
| Lasso Recruitment Ltd | Administrative & support | 2015 |
| Lauren Hilton Pilates Ltd | Other services | 2024 |
| Lava Rocks Ltd | Hospitality | 2019 |
| Lava Stones Limited | Hospitality | 2019 |
| Lb's Boardwalk Cafe Ltd | Hospitality | 2017 |
| LD Fisher Ltd | Transport & logistics | 2014 |
| LE Chien Bakery Ltd | Wholesale & retail | 2025 |
| Lean League Ltd | Arts & recreation | 2025 |
| Leila Bodros Coaching Ltd | Professional & scientific | 2025 |
| Liberate & Elevate Ltd | Education | 2019 |
| Liberty Method Ltd | Manufacturing | 2025 |
| Life in the Saddle Ltd | Professional & scientific | 2017 |
| Ligat Real Estate Ltd | Real estate | 2024 |
| Little Crumb Limited | Hospitality | 2025 |
| Live Karma Yoga CIC | Arts & recreation | 2017 |
| LJR Coaching Limited | Education | 2021 |
| LJR International Sports Ltd | Education | 2025 |
| LMC Motors Ltd | Wholesale & retail | 2020 |
| LMT Removals Limited | Transport & logistics | 2025 |
| LNG Technology Limited | Information & communication | 2025 |
| Lorron Ltd | Hospitality | 2026 |
| Lorron Rentals Ltd | Real estate | 2025 |
| Lotus Cosmetics Ltd | Manufacturing | 2018 |
| Love's Roofing Ltd | Construction | 2022 |
| Loveland Hall Limited | Hospitality | 2022 |
| Loves Lettings Limited | Real estate | 2021 |
| LR Machine Sales Ltd | Wholesale & retail | 2025 |
| LR Performance Coaching Ltd | Education | 2026 |
| Luke J Hutchinson Ltd | Manufacturing | 2025 |
| LXQ Digital Limited | Information & communication | 2021 |
| M.A.N. Services Limited | Wholesale & retail | 2015 |
| MAC Van Ltd | Wholesale & retail | 2022 |
| Macaulay Design Limited | Professional & scientific | 2002 |
| Make and Move Retreats Ltd | Administrative & support | 2026 |
| Make and Move Studio Ltd | Other services | 2026 |
| Make IT Wight Ltd | Wholesale & retail | 2020 |
| Maltmans Limited | Information & communication | 2018 |
| Marlins SLC CIC | Education | 2024 |
| Materialsbypost Ltd | Wholesale & retail | 2015 |
| Matrix Essential Training Alliance Limited | Education | 2000 |
| Mattketing Ltd | Professional & scientific | 2021 |
| Mccarthys Bar and Restaurant Ltd | Hospitality | 2020 |
| Mdaac Properties Ltd | Real estate | 2022 |
| Mediaz Enterprise Limited | Wholesale & retail | 2023 |
| Melville Holdings Ltd | Extraterritorial | 2017 |
| Mersley Alternative Provisions Ltd | Education | 2021 |
| Mewsco 1991 Limited | Households | 1985 |
| Michael Whyte Maintenance Ltd | Manufacturing | 2003 |
| Mike Turner Leisure (IOW) Limited | Other services | 2018 |
| Mike Turner Leisure Limited | Construction | 2021 |