| South West Media Ltd | Information & communication | 2026 |
| South West Property Management Limited | Extraterritorial | 2009 |
| South Wilts Construction Limited | Construction | 2026 |
| South Wilts Grammar School | Education | 2010 |
| South Wilts Mencap | Health & social care | 2005 |
| Southampton Archery Club Limited | Arts & recreation | 2019 |
| Southern Advisory Limited | Professional & scientific | 2005 |
| Southern Computers Limited | Real estate | 2005 |
| Southern Counties Auctioneers Limited | Agriculture & fishing | 1990 |
| Southern Counties Forestry Ltd | Agriculture & fishing | 2015 |
| Southern HAY and Straw Co Limited | Agriculture & fishing | 2018 |
| Southern Quarter Limited | Hospitality | 2020 |
| Southern Smiles Limited | Health & social care | 2003 |
| Southern Transport & Logistics Limited | Transport & logistics | 2020 |
| Southern Tree Care Limited | Agriculture & fishing | 2018 |
| Southern Van Services Limited | Other services | 2007 |
| Southgate Plant Hire Limited | Construction | 2014 |
| Southington Consulting Limited | Professional & scientific | 2026 |
| Southport Smiles Ltd | Health & social care | 2018 |
| Southside Court Limited | Construction | 2003 |
| Southside Tennis Coaching Ltd | Arts & recreation | 2023 |
| Southwest Microcement Ltd | Professional & scientific | 2025 |
| Southwest Padel Ltd | Arts & recreation | 2025 |
| Southwest Property Holdings Limited | Real estate | 2019 |
| Sovereign Court Salisbury Limited | Households | 2003 |
| Spa Parts Europe Limited | Extraterritorial | 2002 |
| Space Design Solutions Limited | Professional & scientific | 2004 |
| Spacebuild (Holding) Limited | Financial services | 2025 |
| Spacebuild Interiors Limited | Administrative & support | 2020 |
| Spacetag Limited | Manufacturing | 2020 |
| Spade Studio Ltd | Information & communication | 2025 |
| Spades Media Limited | Information & communication | 2016 |
| Sparewheels for YOU Limited | Wholesale & retail | 2017 |
| Sparkles Production Limited | Arts & recreation | 2015 |
| Spartan Press Retail Limited | Wholesale & retail | 2002 |
| Spatial Technologies Ltd | Information & communication | 2017 |
| SPD Surveying Services Limited | Professional & scientific | 2014 |
| Spearing & Sons (High Wycombe) Limited | Other services | 2020 |
| Special WOK (Salisbury) Ltd | Hospitality | 2021 |
| Special WOK (SP1) Ltd | Hospitality | 2026 |
| Specialist Telemetry Limited | Professional & scientific | 2008 |
| Speculator Ventures Ltd | Arts & recreation | 2021 |
| Speed Sign Management Ltd | Other services | 2025 |
| Speedcrete C P Limited | Wholesale & retail | 2003 |
| Speedlight Limited | Administrative & support | 1982 |
| Spencer & Merry Ltd | Construction | 2025 |
| Spencer Hall Historic Building Conservation Ltd | Construction | 2020 |
| Spencer Park (Block E) Residents Limited | Households | 2001 |
| Spencer Place Basingstoke Management Limited | Real estate | 2010 |
| SPENCER3D Limited | Education | 2023 |
| SPF Carpentry and Construction Ltd | Construction | 2022 |
| Sphere for Good Limited | Other services | 2026 |
| Spider International Business Development Limited | Professional & scientific | 2018 |
| Spindleberry Investments Ltd | Real estate | 2025 |
| Spinnaker Westbury Residents Management Company Limited | Households | 2019 |
| Spiral Compass LLP | | 2026 |
| Spire Building Control Services Limited | Construction | 2011 |
| Spire Coaching and Training Ltd | Professional & scientific | 2018 |
| Spire Contractors Ltd | Professional & scientific | 2019 |
| Spire Decorating Limited | Construction | 2017 |
| Spire Digital (Salisbury) Limited | Information & communication | 2018 |
| Spire Digital Limited | Information & communication | 2016 |
| Spire Edge Tours Ltd | Administrative & support | 2026 |
| Spire Flooring Limited | Wholesale & retail | 2009 |
| Spire Gate Automation Limited | Construction | 2022 |
| Spire Glass Holdings Limited | Financial services | 2024 |
| Spire Glass Ltd | Construction | 2001 |
| Spire Hand Car Wash Limited | Wholesale & retail | 2024 |
| Spire Homecare 2016 Limited | Health & social care | 2016 |
| Spire Imports Ltd | Wholesale & retail | 2019 |
| Spire Inclusion Limited | Public administration | 2024 |
| Spire Independent Financial Planning Ltd | Financial services | 2017 |
| Spire Light Limited | Information & communication | 2026 |
| Spire Medical Supplies Limited | Wholesale & retail | 2017 |
| Spire Memorials Limited | Other services | 2011 |
| Spire Mews Management Company Limited | Real estate | 2013 |
| Spire Mortgages Limited | Administrative & support | 2007 |
| Spire Printing Limited | Information & communication | 2005 |
| Spire Rehabilitation Limited | Administrative & support | 2016 |
| Spire Removals Limited | Transport & logistics | 2013 |
| Spire Rentals Limited | Households | 2024 |
| Spire Scaffolding Safe Acces Limited | Construction | 2025 |
| Spire Surveys Ltd | Construction | 2019 |
| Spire Telecom Limited | Information & communication | 2023 |
| Spire Travel Limited | Transport & logistics | 2007 |
| Spire Ultrasound Ltd | Health & social care | 2025 |
| Spire View Accounts Ltd | Professional & scientific | 2025 |
| Spirit Quarters Residents Management Company Limited | Households | 2017 |
| Sporn Construction Limited | Construction | 2001 |
| Sportrax Technology Ltd | Wholesale & retail | 2013 |
| Sports Online Limited | Wholesale & retail | 2004 |
| Spotless Shine Cleaning Services Limited | Administrative & support | 2024 |
| Spotlight Consultancy Limited | Other services | 2020 |
| Spring Meadow (Witney) Management Company Limited | Households | 2015 |
| Spring Meadows (Fontmell Magna) Estate Management Limited | Households | 2022 |
| Springate Limited | Administrative & support | 2006 |
| Springbok Heating Services Ltd | Construction | 2021 |
| Springbourne Barn Limited | Construction | 2021 |
| Springbourne Cottage Limited | Construction | 2021 |
| Springcore Limited | Information & communication | 2025 |