| DP & LJ Attew Ltd | Professional & scientific | 2023 |
| DP & LJ Attew Trading Ltd | Construction | 2024 |
| DPH Mechanical Services Limited | Construction | 2009 |
| DPS PMC Limited | Extraterritorial | 2015 |
| DR Hannah Reidy Limited | Health & social care | 2024 |
| Dr.Nmb Consultancy Limited | Professional & scientific | 2025 |
| Dragoni Limited | Financial services | 2020 |
| Dragoni Properties Limited | Real estate | 2020 |
| Drain Burst Ltd | Construction | 2017 |
| Draper LEE Associates Limited | Professional & scientific | 2003 |
| Dream Spice Limited | Hospitality | 2019 |
| Driftend Limited | Information & communication | 2015 |
| Dronewatch Limited | Extraterritorial | 2016 |
| Drop Bear Media Limited | Professional & scientific | 2019 |
| Drowned in Sound Limited | Information & communication | 2025 |
| DT Design Limited | Manufacturing | 2004 |
| Dtco Finance Limited | Extraterritorial | 2020 |
| Duck Street Management Company Limited | Real estate | 2009 |
| Dullingham Developments Limited | Construction | 1972 |
| Dupreys Hair and Beauty Limited | Other services | 2025 |
| Dyneq Medical Limited | Professional & scientific | 2023 |
| D’Arcy’S Lounge and Bar Ltd | Hospitality | 2025 |
| E & H Allan Ltd | Wholesale & retail | 2025 |
| E P Direct Limited | Other services | 1990 |
| E-Max Bikes Ltd | Administrative & support | 2021 |
| E.W.Nell Limited | Professional & scientific | 1933 |
| Eabs (SW) Ltd | Administrative & support | 2020 |
| Ealing Wine Cellars Ltd | Wholesale & retail | 2020 |
| Earth and Arbor Ltd | Construction | 2025 |
| Earthflux Limited | Extraterritorial | 2023 |
| East African Trading Co. Ltd | Wholesale & retail | 2012 |
| East Anglia Urgent Care Ltd | Health & social care | 2025 |
| East Anglian Tractors Limited | Wholesale & retail | 1986 |
| East Art Gallery Ltd | Wholesale & retail | 2023 |
| East London Management Limited | Real estate | 2016 |
| Eastacre (Management) Limited | Households | 1988 |
| Eastgate Management Company Limited | Extraterritorial | 2016 |
| Easton Hall Solutions Limited | Information & communication | 2022 |
| Easykey Limited | Information & communication | 2003 |
| Eauso Fragrances Ltd | Wholesale & retail | 2025 |
| Eazi Accommodation Ltd | Real estate | 2024 |
| ECB Training LLP | | 2025 |
| Ecco Wash Limited | Administrative & support | 2024 |
| Echo Element Limited | Wholesale & retail | 2024 |
| Ecila Global Limited | Administrative & support | 2025 |
| Ecim Holdings Limited | Financial services | 2024 |
| Eco Enterprise Retail Limited | Wholesale & retail | 2024 |
| Eco Solutions Plumbing Ltd | Construction | 2025 |
| Ecogreenrm Limited | Energy | 2022 |
| Ecommerce Technologies Ltd | Financial services | 2017 |
| Edam Investments Limited | Financial services | 2017 |
| Eddy Road Consulting Limited | Professional & scientific | 2020 |
| Eden Lake (Saffron Walden) Ltd | Wholesale & retail | 2013 |
| Eden Rock Construction Limited | Real estate | 2017 |
| Eden Sier Property Limited | Real estate | 2025 |
| EDO SAN Ltd | Administrative & support | 2014 |
| EEP Filters Limited | Manufacturing | 2020 |
| EFE Mobile Barber Ltd | Other services | 2020 |
| Efficacy EVA C.I.C. | Professional & scientific | 2009 |
| Eggersmann Kuchen LLP | | 2017 |
| EH Property Solutions Ltd | Administrative & support | 2023 |
| Eide Limited | Wholesale & retail | 2024 |
| Eightcreate Limited | Professional & scientific | 2020 |
| Eikonika Limited | Professional & scientific | 1995 |
| EJ Cleaning Solutions Ltd | Administrative & support | 2025 |
| Ejsolutions Ltd | Administrative & support | 2025 |
| Elal DEV Limited | Information & communication | 2026 |
| Elcon Electrics Ltd | Construction | 2025 |
| Elder Street Cafe & Deli Ltd | Hospitality | 2015 |
| Elder Street Food Ltd | Hospitality | 2025 |
| Elevated Beauty Limited | Other services | 2021 |
| Elisal Investments Ltd | Administrative & support | 2024 |
| Elizabeth Close Management Company Limited | Households | 1993 |
| Elliott Securities London Ltd | Manufacturing | 2013 |
| Ellise Limited | Administrative & support | 2011 |
| Elmdon Farms Limited | Agriculture & fishing | 1947 |
| Elms Lawn Tennis Club Limited | Arts & recreation | 2024 |
| Elmsandco Limited | Manufacturing | 2018 |
| Elmscroft Limited | Manufacturing | 1987 |
| Elphick Digital Ltd | Information & communication | 2024 |
| Embedded Systems Consultants Limited | Information & communication | 1999 |
| Emery House Property Limited | Real estate | 2013 |
| Emery House Trading Company Limited | Wholesale & retail | 2016 |
| Emma Pearson Design Limited | Professional & scientific | 2006 |
| Emma Rhoda Limited | Health & social care | 2023 |
| Empathic Mind Limited | Professional & scientific | 2013 |
| Emporian Media Limited | Professional & scientific | 2025 |
| Enbe Developments Ltd | Real estate | 2020 |
| ENC Electrical Limited | Construction | 2004 |
| ENC Media Limited | Information & communication | 2024 |
| Endless Wire Events Limited | Hospitality | 2012 |
| Energy Booker Limited | Wholesale & retail | 2011 |
| Enfield Business Services Limited | Professional & scientific | 2003 |
| Engelmann Farms Limited | Agriculture & fishing | 1961 |
| Engineering Resources Limited | Professional & scientific | 1997 |
| Enhanced Operating Systems Limited | Information & communication | 1995 |
| Enomis Limited | Administrative & support | 2001 |
| Enterprise East Group C.I.C. | Administrative & support | 2017 |
| Entertainment Industries Limited | Administrative & support | 2012 |
| Entheos Consulting Ltd | Other services | 2015 |