| Larks Mead Consulting Limited | Information & communication | 2020 |
| Lateral Living IOW Ltd | Construction | 2023 |
| Latimer Road Limited | Households | 2001 |
| Launderama Ltd | Other services | 2021 |
| Lavell & Co Limited | Financial services | 2003 |
| Layerbee 3D Forge Ltd | Manufacturing | 2025 |
| LC Music UK Limited | Information & communication | 2026 |
| Leisureiq Ltd | Professional & scientific | 2025 |
| Lemon Valley Holidays LLP | | 2016 |
| Letterpress Design Limited | Manufacturing | 2025 |
| Libra Design Associates Limited | Professional & scientific | 2002 |
| Lillington House Management Limited | Households | 1990 |
| Lillyjames Limited | Real estate | 2014 |
| Lilot LLP | | 2024 |
| Lily Oliver Limited | Professional & scientific | 2008 |
| Linear Services Ltd | Construction | 2026 |
| Liquidset Ltd | Information & communication | 2002 |
| Lnroofingservices Limited | Construction | 2025 |
| Lobster Locations Ltd | Arts & recreation | 2017 |
| London Hanover Associates Limited | Administrative & support | 1981 |
| London Parking Solutions Ltd | Administrative & support | 2007 |
| Lost Lover Boutique Limited | Wholesale & retail | 2023 |
| Lucky H Limited | Hospitality | 2014 |
| Luke Randall Bespoke Lead Work and Roofing Ltd | Construction | 2022 |
| Lushington Hill Bodyshop Limited | Wholesale & retail | 2000 |
| Lushington Hill Garage Limited | Wholesale & retail | 1988 |
| Lyme Bay Press Ltd | Manufacturing | 2014 |
| M&H Pro-Vestments Ltd | Construction | 2025 |
| M.P.R Stonemasonry & Lime Pointing Ltd | Manufacturing | 2025 |
| M.S. Holdings (I.O.W.) Limited | Wholesale & retail | 1977 |
| Made & Worn Ltd. | Wholesale & retail | 2018 |
| Maher Ross Ltd | Real estate | 2007 |
| Making and Creating Ltd | Professional & scientific | 2023 |
| Malgraphy Limited | Professional & scientific | 2024 |
| Marinauto UK Ltd | Manufacturing | 2020 |
| Mark Marcus Consultancy Limited | Information & communication | 2015 |
| Marlborough Road (IW) Management Company Limited | Households | 2020 |
| Mary Bell Court Residents Company Limited | Administrative & support | 2009 |
| Massala Bowl Limited | Hospitality | 2022 |
| Matt Smith Leadership Consultancy Limited | Education | 2010 |
| Matthew Coughlan Consulting Limited | Information & communication | 2013 |
| Matthew D Jones Architectural Services Limited | Professional & scientific | 2005 |
| Mechanical Electrical GAS Assured Systems Ltd | Professional & scientific | 2022 |
| Medalvault Ltd | Arts & recreation | 2026 |
| Medina Construction Limited | Construction | 2015 |
| Mefm Properties Ltd | Real estate | 2024 |
| Mega Systems Limited | Wholesale & retail | 2014 |
| MEN Only IOW Community Interest Company | Health & social care | 2022 |
| Mermaid Entertainment Company Ltd | Arts & recreation | 2025 |
| Mermaid Medics Limited | Health & social care | 2022 |
| Merrydale Residential Care Home Limited | Health & social care | 2012 |
| Merstone Building Fabric Maintenance Ltd | Construction | 2023 |
| Metrocharm Limited | Real estate | 1984 |
| MGN Homes Ltd | Real estate | 2025 |
| Michael Barlow Financial Limited | Financial services | 2020 |
| Michelle Ayers IT Consultancy Ltd | Information & communication | 2025 |
| Miga Chinese Limited | Hospitality | 2022 |
| Milena Taxi Ltd | Transport & logistics | 2026 |
| Millward Group Ltd | Construction | 2021 |
| Mindset Bros IOW CIC | Health & social care | 2025 |
| Mindset Bros Ltd | Wholesale & retail | 2024 |
| Miniport Ships Ltd | Arts & recreation | 2009 |
| Mjgholgado Limited | Professional & scientific | 2015 |
| MJJ Aviation Ltd | Manufacturing | 2023 |
| Mobile Fashion Ltd | Wholesale & retail | 2018 |
| Modavcress Ltd | Wholesale & retail | 2022 |
| Monkey Tree Limited | Real estate | 2018 |
| Monkeyfishmaughan Ltd | Construction | 2023 |
| Monkton Street Management Company Limited | Households | 2003 |
| Monster Channel Ltd | Wholesale & retail | 2022 |
| Morgan Recycling Ltd | Other services | 2025 |
| Morris Geotech R&D Ltd | Professional & scientific | 2021 |
| Morton Tax Ltd | Professional & scientific | 2024 |
| Mossie Estates Ltd | Real estate | 2023 |
| Mountain Way Limited | Information & communication | 1997 |
| MPL Brickwork IOW Limited | Construction | 2023 |
| MRC Composite Limited | Manufacturing | 2019 |
| Mrtl Limited | Administrative & support | 2003 |
| Msoffshore Limited | Manufacturing | 2026 |
| Murphy Social Limited | Other services | 2023 |
| MW Electrical Services (IOW) Limited | Construction | 2017 |
| Mystical Soul Sanctuary Ltd. | Education | 2023 |
| N & P Buildings LLP | | 2008 |
| N Helps Ltd | Other services | 2021 |
| N-Data Limited | Information & communication | 2004 |
| NAB House Management Company Limited | Administrative & support | 2014 |
| Nanhurst Stable Flats Ltd | Real estate | 2017 |
| Natural Beauty Limited | Other services | 2026 |
| Natural Neutral Ltd. | Wholesale & retail | 2015 |
| Neater Accounts Limited | Professional & scientific | 2000 |
| Negatech Limited | Hospitality | 2000 |
| New Media Europe Ltd. | Real estate | 2015 |
| New Ventures Worldwide CIC | Education | 2021 |
| Newnham Wood Ltd | Agriculture & fishing | 2024 |
| Nine Bytes Ltd | Information & communication | 2021 |
| NJR Corporate Limited | Professional & scientific | 2013 |
| NMN Advisory Ltd | Professional & scientific | 2023 |
| NO.3 Beachlands Limited | Hospitality | 2021 |
| Noble Contractors IOW Limited | Manufacturing | 2023 |
| Noir & Rose Ltd | Wholesale & retail | 2026 |