| ST Driver Solutions Ltd | Administrative & support | 2026 |
| ST Erik Trans Ltd | Manufacturing | 2024 |
| ST Michaels Fish Bar Ltd | Hospitality | 2020 |
| ST6 Limited | Real estate | 2010 |
| Staff Rite Recruitment Limited | Administrative & support | 2017 |
| STAFF4DSHIRE Developments Limited | Professional & scientific | 2022 |
| STAFF4DSHIRE Groundworks & Brickwork Ltd | Construction | 2021 |
| STAFF4DSHIRE Hire Limited | Administrative & support | 2026 |
| STAFF4DSHIRE Properties Ltd | Households | 2022 |
| STAFF4DSHIRE Roofing Specialists Ltd | Construction | 2023 |
| Stafford Brook Stables Limited | Agriculture & fishing | 2002 |
| Stafford Drains Limited | Administrative & support | 2013 |
| Stafford Education Limited | Education | 2017 |
| Stafford Web Studio Limited | Information & communication | 2012 |
| Staffordshire Autohaus Ltd | Wholesale & retail | 2026 |
| Staffordshire Brick Tinting Services Ltd | Construction | 2024 |
| Staffordshire Fruits Limited Liability Partnership | | 2015 |
| Staffordshire Green Energy Limited | Construction | 2023 |
| Staffordshire Home Renovations Ltd | Manufacturing | 2025 |
| Staffordshire Pets Ltd | Wholesale & retail | 2021 |
| Staffordshire Tech Limited | Information & communication | 2023 |
| Staffs Properties Ltd | Construction | 2024 |
| Staffs Smart Energy Limited | Other services | 2022 |
| Staffs Timber Ltd | Agriculture & fishing | 2023 |
| Stag Pine (UK) Ltd | Manufacturing | 2002 |
| Stansbie Building Ltd | Construction | 2021 |
| Starburger Rugeley Limited | Hospitality | 2018 |
| START2FINISH Construction Ltd | Construction | 2026 |
| Startup Coach Ltd | Professional & scientific | 2021 |
| Stem Designs Limited | Construction | 2017 |
| Stem Property Limited | Real estate | 2024 |
| Stemic Limited | Manufacturing | 2012 |
| Step-By-Step Financial Planners Limited | Financial services | 2010 |
| Stephanie Hollinshead Racing Limited | Arts & recreation | 2013 |
| Stephen Sylvester Plasterers Limited | Construction | 2003 |
| Sterlage Limited | Manufacturing | 1943 |
| Stimpson Estates Ltd | Real estate | 2026 |
| Stirling Global Consultancy Limited | Professional & scientific | 2009 |
| Stirling Sales Agencies Limited | Wholesale & retail | 2009 |
| STL Securities Ltd | Administrative & support | 2024 |
| Stockeasey Mortgages Limited | Financial services | 2006 |
| Stoke Fast Food Supplies Ltd | Wholesale & retail | 2021 |
| Stonehart Corporation Limited | Real estate | 2024 |
| Strada Romana Limited | Hospitality | 2012 |
| Streo Limited | Administrative & support | 2023 |
| Stria Group Limited | Construction | 2025 |
| Stria Pavement Inspection Limited | Construction | 2026 |
| Strobe Construction Limited | Construction | 2010 |
| Stronghold Audio Limited | Arts & recreation | 2016 |
| Struct Marketing Ltd | Information & communication | 2024 |
| Stubbrook Developments Ltd | Construction | 2002 |
| Studentlife Furniture Ltd | Administrative & support | 2009 |
| STW Consultants Limited | Construction | 2022 |
| Style OUR Home Ltd | Wholesale & retail | 2007 |
| Success Science Ltd | Information & communication | 2022 |
| Summerfields Nurseries (Catton) Limited | Agriculture & fishing | 2007 |
| Sun 1400 Limited | Professional & scientific | 2009 |
| Sunny Rank Ltd | Information & communication | 2025 |
| Superior Heat Pumps Ltd | Construction | 2015 |
| Supplyco Limited | Professional & scientific | 2005 |
| Suretech IT Limited | Information & communication | 2013 |
| Sustineri Future Limited | Professional & scientific | 2024 |
| Sutton Bollards & Gates Ltd | Construction | 2024 |
| Sutton Grosvenor Limited | Hospitality | 2008 |
| SW Tour Services Ltd | Administrative & support | 2021 |
| Sylva Heart CIC | Agriculture & fishing | 2025 |
| Synergia Wealth Ltd | Financial services | 2014 |
| Synergy Print Management Services Limited | Information & communication | 2000 |
| Synergy Strength Academy Ltd | Arts & recreation | 2025 |
| Syntects Ltd | Agriculture & fishing | 2023 |
| Systems Valley Limited | Information & communication | 2005 |
| T & I Developments Limited | Construction | 2007 |
| T & J Holdings (Staffs) Limited | Construction | 2025 |
| T & S Carpets and Beds Limited | Wholesale & retail | 2012 |
| T & S Transport Services (Rugeley) Limited | Transport & logistics | 2007 |
| T Birkin Limited | Construction | 2007 |
| T Richards Ltd | Professional & scientific | 2025 |
| T&H Construction Limited | Construction | 2009 |
| T.A Robathan Ltd | Transport & logistics | 2023 |
| T115LEY Limited | Real estate | 2016 |
| T150 Energy Ltd | Wholesale & retail | 2019 |
| TAG Metals and Export Limited | Wholesale & retail | 2022 |
| Tailor Made Systems Limited | Manufacturing | 1984 |
| Tall Poppies Interiors Limited | Professional & scientific | 2009 |
| Tanic Sun Ltd | Other services | 2022 |
| Target Fire and Security Ltd | Construction | 2026 |
| Taurus SL Limited | Wholesale & retail | 2018 |
| Taxcor Limited | Real estate | 1986 |
| Taylor Made Lettings (Staffordshire) Ltd | Real estate | 2026 |
| Taylor Maids (UK) Limited | Administrative & support | 2002 |
| TBS Cladding Solutions Ltd | Construction | 2018 |
| TBS Group Holdings Ltd | Wholesale & retail | 2018 |
| TBS Specialist Products Ltd | Wholesale & retail | 2013 |
| Tcdms Ltd | Construction | 2025 |
| TDM Installs Ltd | Information & communication | 2025 |
| Team Laburnum Ltd | Professional & scientific | 2020 |
| Techcare Ltd | Information & communication | 2010 |
| Technical Installation Services Ltd | Construction | 2025 |
| Technology Trish Ltd | Education | 2006 |
| TED Hawthorne Catering Limited | Hospitality | 2002 |