| Specialised Sports Ltd | Arts & recreation | 2022 |
| Specialist Electrical Cleaning Services Ltd | Administrative & support | 2025 |
| Specialist Labour Supplies Ltd | Construction | 2024 |
| Specialist Trade Solutions Ltd | Wholesale & retail | 2024 |
| Specs by Post Limited | Wholesale & retail | 2005 |
| Spectrum Coatings (South Yorkshire) Limited | Manufacturing | 2003 |
| Spectrum Futures C.I.C. | Professional & scientific | 2011 |
| Spectrum Innovations Limited | Information & communication | 2025 |
| Speedwell Office Systems Limited | Wholesale & retail | 2013 |
| Spekaf Ltd | Health & social care | 2017 |
| Spencers of Bawtry Ltd | Wholesale & retail | 2025 |
| Spice Sheffield Limited | Real estate | 2018 |
| Spicy Greek Ltd | Hospitality | 2019 |
| Spill Defence Manufacturing Ltd | Manufacturing | 2018 |
| Spiralock of Europe Ltd. | Extraterritorial | 1996 |
| Spiritual Tours Ltd | Manufacturing | 2025 |
| SPK Structural Limited | Professional & scientific | 2017 |
| Splat Web Solutions Ltd | Professional & scientific | 2022 |
| SPLATTS2711 Limited | Construction | 2018 |
| Splice Works Limited | Information & communication | 2025 |
| Sport Vans UK Ltd | Wholesale & retail | 2023 |
| Spot on Fashion Ltd | Wholesale & retail | 2026 |
| Spotwood & Sons Property Ltd | Real estate | 2023 |
| SPR Entertainment Ltd | Hospitality | 2025 |
| Spring Traders Limited | Extraterritorial | 2024 |
| Springboard Consultants Limited | Information & communication | 2025 |
| Springboard Helpers Limited | Health & social care | 2021 |
| Springboard Horizons Limited | Health & social care | 2025 |
| Springboard Trading Limited | Wholesale & retail | 2025 |
| Springvale Canine Creche Limited | Other services | 2013 |
| Springwood Fire Protection & Plastering Limited | Public administration | 2022 |
| Springwood Property Services Ltd | Construction | 2026 |
| Springwood Vehicle Logistics Ltd | Transport & logistics | 2022 |
| Sprint Finish Couriers (South Yorks) Limited | Transport & logistics | 2011 |
| Sprint Lifting Equipment Ltd | Manufacturing | 2025 |
| Spro (UK) Limited | Construction | 2011 |
| Spro Hire Limited | Construction | 2016 |
| Spro Properties Limited | Real estate | 2016 |
| Sprotbrough Tea Rooms Ltd | Hospitality | 2016 |
| SPS Accident Repair Centre Ltd | Wholesale & retail | 2025 |
| SPS Safenet Ltd | Construction | 2011 |
| SPS Yorkshire Properties Limited | Real estate | 2022 |
| Spvin Ltd | Wholesale & retail | 2014 |
| SQP Live Limited | Arts & recreation | 2015 |
| Squad Training Limited | Arts & recreation | 2018 |
| Squareone Laser Clinic Ltd | Other services | 2023 |
| SQUEAKYCLEAN4U Ltd | Administrative & support | 2024 |
| Squires Rail & Civil Engineering Ltd | Construction | 2019 |
| SR Mortgage Services Limited | Financial services | 2013 |
| SR Plastering Limited | Construction | 2018 |
| Srayan Ltd | Wholesale & retail | 2024 |
| SRB Roofing Limited | Wholesale & retail | 2006 |
| Sretlaw Innovations Limited | Professional & scientific | 2010 |
| SRH Plumbing & Heating Ltd | Construction | 2018 |
| SRI Dhanalakshmi Healthcare Limited | Wholesale & retail | 2025 |
| SRI Lakshmi Healthcare Limited | Wholesale & retail | 2022 |
| SRI Lakshmi Hills Ltd | Real estate | 2025 |
| SRI Lakshmi Homes Limited | Real estate | 2022 |
| SRI Nidhi Healthcare Limited | Wholesale & retail | 2022 |
| SRI SAI Healthcare Limited | Wholesale & retail | 2022 |
| SRI SRI MED Ltd | Professional & scientific | 2017 |
| SRI Vibha Ltd | Wholesale & retail | 2020 |
| Srijhal Infra Ltd | Real estate | 2025 |
| SRK Crafting Earth Ltd | Manufacturing | 2023 |
| SRM Constructors Limited | Construction | 2021 |
| Srsd Limited | Information & communication | 2020 |
| Srusa Soccer UK Ltd | Arts & recreation | 2022 |
| SS JDM Auto Ltd | Wholesale & retail | 2023 |
| SS Painting and Decorating Specialists Ltd | Construction | 2022 |
| SS Properties 2022 Limited | Real estate | 2022 |
| SS Retail Services Limited | Wholesale & retail | 2026 |
| SS Travel Ltd | Transport & logistics | 2024 |
| Ss&T Logistics Ltd | Transport & logistics | 2025 |
| Ssaas Express Ltd | Hospitality | 2016 |
| SSB Rail Ltd | Construction | 2016 |
| Ssdm Healthcare Private Limited | Health & social care | 2013 |
| SSG Prestige Ltd | Wholesale & retail | 2020 |
| SSM Recovery Ltd | Wholesale & retail | 2023 |
| SSS Healthcare Limited | Wholesale & retail | 2022 |
| ST Cladding Ltd | Construction | 2024 |
| ST Francis Catholic Multi Academy Trust | Education | 2021 |
| ST Peters Pharmacy (Burnley) Limited | Health & social care | 2006 |
| ST. Ermyns Ltd | Administrative & support | 2014 |
| Stable Homes & Assistance Limited | Health & social care | 2026 |
| Stable Scaffolding Limited | Construction | 2022 |
| Stables Heating (Contractors) Limited | Construction | 1997 |
| Stables Scaffolding Solutions Limited | Construction | 2024 |
| Stac Fitness Ltd | Education | 2024 |
| Stacey Communications Ltd | Health & social care | 2023 |
| Staff Training Company Ltd | Professional & scientific | 2015 |
| Stafforce Limited | Extraterritorial | 1997 |
| Staffordshire Windows & Conservatories Ltd | Construction | 2023 |
| Stag Mobility Ltd | Wholesale & retail | 2024 |
| Stag Superstore Ltd | Wholesale & retail | 2025 |
| Staging Events Ltd | Administrative & support | 2014 |
| Stainrod Enterprises Limited | Real estate | 2024 |
| Stainton Hall Developments Limited | Construction | 2001 |
| Stair Kraft Limited | Professional & scientific | 2011 |
| Staircase & Handrail Ltd | Manufacturing | 2022 |
| Staircases by John Lawson Ltd | Construction | 2017 |