| DT Financial Services Ltd | Financial services | 2017 |
| DT Interiors Ltd | Construction | 2018 |
| DT Windsurf Coach Ltd | Arts & recreation | 2007 |
| DTO Communications Limited | Information & communication | 2005 |
| Dudbert Holdings Limited | Financial services | 2026 |
| Duke Readings Holdings Limited | Financial services | 2014 |
| Dulay Financial Services Limited | Financial services | 2003 |
| Dunne Roberts Limited | Manufacturing | 2004 |
| Dunwood Meadows Ltd | Hospitality | 2023 |
| Duroc Media Limited | Professional & scientific | 2001 |
| Duroc Music Limited | Arts & recreation | 2019 |
| Dushan Thavarajah Ltd | Health & social care | 2019 |
| Duvine Limited | Manufacturing | 2006 |
| DWC Group Ltd | Professional & scientific | 2024 |
| Dwell & Connection Limited | Real estate | 2024 |
| Dxtrus Limited | Other services | 2024 |
| Dymond Developments Ltd | Construction | 2017 |
| Dyson Contracting Limited | Construction | 2007 |
| E C M Management Services Limited | Professional & scientific | 2006 |
| E&E Alibaba Limited | Professional & scientific | 2016 |
| E&S Holloway Limited | Real estate | 2022 |
| E1 Matters Limited | Professional & scientific | 2017 |
| EA Car Sales Ltd | Wholesale & retail | 2016 |
| Eagle Engineering Services Limited | Professional & scientific | 2023 |
| Early Years Maintenance Ltd | Administrative & support | 2025 |
| Earlyitem Limited | Arts & recreation | 1998 |
| Easli Limited | Wholesale & retail | 2026 |
| Easterbrook Property Rental LLP | | 2023 |
| Eastwell Photographic Limited | Professional & scientific | 2022 |
| Eastwestern Properties Ltd | Real estate | 2022 |
| EC Wellbeing Ltd | Information & communication | 2024 |
| Eccp Limited | Administrative & support | 2020 |
| Echovista Ultrasound Surface Cleaning Ltd | Professional & scientific | 2023 |
| Ecole DE Pole London LLP | | 2023 |
| Ecole DE Pole Ltd | Arts & recreation | 2024 |
| Ecological Consultancy Services Ltd | Other services | 2012 |
| Ecotech Build Systems Limited | Manufacturing | 2020 |
| ECS Spinecraft Limited | Health & social care | 2019 |
| EDE Plumbing & Heating Ltd | Construction | 2015 |
| Edify Group Ltd | Financial services | 2021 |
| Edify Properties Ltd | Real estate | 2021 |
| Edify SA Ltd | Administrative & support | 2021 |
| Editionldn Ltd | Households | 2020 |
| Edwards Geophysical Ltd | Professional & scientific | 2006 |
| Effluent Solutions Ltd | Construction | 2022 |
| EFS Engineering Ltd | Professional & scientific | 2023 |
| Egd-Services Ltd. | Administrative & support | 2010 |
| EIS Services Group Limited | Construction | 2005 |
| EJS Print Finishers Limited | Manufacturing | 2009 |
| EJW Medical Limited | Health & social care | 2018 |
| Electrical & Electronic Fire & Security Solutions Limited | Construction | 2006 |
| Elegance Hair Limited | Other services | 2013 |
| Elegant Wines Limited | Hospitality | 2013 |
| Elemental Club Limited | Real estate | 2016 |
| Elite Cars Europe Limited | Administrative & support | 2012 |
| Elite Estate Group Limited | Real estate | 2021 |
| Eliza Marti Ltd | Manufacturing | 2025 |
| Ella MCK Creations Limited | Arts & recreation | 2020 |
| ELM Anaesthesia and Wellbeing Limited | Health & social care | 2025 |
| ELUMIN8 Limited | Manufacturing | 2009 |
| Elysia Medical Legal Services Limited | Health & social care | 2009 |
| Elystan Court RTM Company Ltd | Households | 2022 |
| Emax Homes Limited | Real estate | 2022 |
| Emax Motorsport Limited | Arts & recreation | 2011 |
| Ember CM & Therapy Services Limited | Health & social care | 2026 |
| Embley Equestrian Services Ltd | Agriculture & fishing | 2019 |
| Embley Holdings Limited | Professional & scientific | 2025 |
| Embocure Ltd | Health & social care | 2026 |
| Emer Farm Limited | Agriculture & fishing | 2016 |
| Emery Soft Fruits Limited | Agriculture & fishing | 2021 |
| EMH Motorhome Hire Ltd | Other services | 2025 |
| Emissario Consulting Limited | Information & communication | 2007 |
| Emma Merrills Holistic Therapies Ltd | Other services | 2022 |
| Emma Thomson Consulting Ltd | Professional & scientific | 2012 |
| Emphasis Limited | Administrative & support | 2009 |
| Empire Barbers Limited | Other services | 2016 |
| Employment Partners (South West) Limited | Administrative & support | 2016 |
| Employment Partners Group (Holdings) Ltd | Financial services | 2021 |
| Employment Partners Limited | Administrative & support | 2015 |
| Empowered EVE Ltd | Other services | 2024 |
| Emvia Ltd | Professional & scientific | 2023 |
| EN Press Limited | Manufacturing | 1991 |
| Enchanted Nanny Ltd | Health & social care | 2017 |
| Endersleigh Court RTM Company Limited | Households | 2013 |
| Endodent Limited | Health & social care | 2022 |
| Engine House RTM Company Ltd | Households | 2026 |
| Engineering 360 Ltd | Professional & scientific | 2024 |
| Engineering Technology Applications Limited | Manufacturing | 1996 |
| Entire Doors Ltd | Construction | 2019 |
| Enviroaqua Ltd | Construction | 2014 |
| Enzyme Energy Limited | Manufacturing | 2010 |
| Eojb Limited | Health & social care | 2014 |
| Epidermed Ltd | Health & social care | 2025 |
| Epona Holdings Ltd | Financial services | 2024 |
| Equestrian Enterprises Ltd | Other services | 2016 |
| Equiland Partners (UK) Limited | Real estate | 2023 |
| Equine RUG Rescue Ltd | Other services | 2022 |
| Equinited Limited | Wholesale & retail | 2021 |
| Equity Chambers RTM Company Ltd | Households | 2025 |
| Equity Will- Writing Ltd | Professional & scientific | 1998 |