| Dovedale Damp Company Limited | Construction | 1990 |
| Downstone Limited | Real estate | 1962 |
| Dpfp LLP | | 2014 |
| Drakehouse Court Property Management Limited | Professional & scientific | 2007 |
| DRIVER68 Ltd | Transport & logistics | 2026 |
| DS Plastering and Spray Rendering Ltd | Construction | 2019 |
| DS Spray Rendering Ltd | Construction | 2026 |
| DW Kitchens & Bathrooms Ltd | Construction | 2026 |
| DWF Home Improvements Ltd | Construction | 2025 |
| E & H Midlands Limited | Real estate | 2017 |
| E.J Coachworks Ltd | Wholesale & retail | 2025 |
| Earwig Academic Reporting Limited | Education | 2012 |
| East Midlands Boiler Servicing Limited | Construction | 2021 |
| East Midlands JIU Jitsu Association Ltd | Arts & recreation | 2010 |
| East Midlands Realisations 2025 Limited | Professional & scientific | 2020 |
| Edenfield Developments Ltd | Construction | 2022 |
| Ef&N Limited | Professional & scientific | 2011 |
| EIS Developments Ltd | Real estate | 2022 |
| Ejes Ltd | Construction | 2023 |
| EK Consultancy 1 Ltd | Professional & scientific | 2025 |
| Elite Barber Derby Ltd | Other services | 2025 |
| ELL Distributions Ltd | Wholesale & retail | 2026 |
| Ellis-Fermor & Negus Limited | Professional & scientific | 2011 |
| Emergency Exit Solutions Limited | Administrative & support | 2020 |
| Energem Transmission and Distribution Services Ltd | Wholesale & retail | 2025 |
| Engage and Inspire Ltd | Professional & scientific | 2024 |
| Engine House Motors Ltd | Wholesale & retail | 2024 |
| Engineering Parts Ltd | Manufacturing | 2025 |
| ENS Electrical Ltd | Construction | 2026 |
| Enviro Trac Ltd | Construction | 2008 |
| Erhardt Holdings Limited | Financial services | 2025 |
| Esther Flynn Speech and Language Therapy Limited | Health & social care | 2019 |
| Europe Wired Limited | Construction | 2009 |
| European Vehicle Tracking Limited | Extraterritorial | 2017 |
| Evolve Group of Companies Ltd | Financial services | 2025 |
| Evolve Integrated Solutions Ltd | Construction | 2019 |
| Exact Plant Hire Limited | Administrative & support | 2018 |
| Exebern Properties Limited | Construction | 2015 |
| EXPERIENCE2INSPIRE Limited | Professional & scientific | 2015 |
| Eyres Holding Limited | Financial services | 2024 |
| F.P UK Developments Limited | Financial services | 2024 |
| Facade Design Solutions Ltd | Professional & scientific | 2026 |
| Facilis Logistics Limited | Transport & logistics | 2022 |
| Facilis Logistics Solutions Limited | Administrative & support | 2026 |
| Fadeni Properties Ltd | Real estate | 2022 |
| Family Well-Being & Enterprise Ltd | Administrative & support | 2024 |
| Fenwicks Fencing Ltd | Manufacturing | 2018 |
| Fiannova Limited | Wholesale & retail | 2020 |
| Filigree Group Limited | Manufacturing | 2024 |
| Filigree Holdings Limited | Manufacturing | 2006 |
| Filigree Limited | Professional & scientific | 1990 |
| Filigree Textiles Limited | Professional & scientific | 1991 |
| Fiona Gavin Performance Coaching and Consultancy Ltd | Administrative & support | 2025 |
| First Safety Limited | Wholesale & retail | 2008 |
| Firstview Lettings Limited | Professional & scientific | 2004 |
| Fishpond (Matlock Bath) Ltd | Hospitality | 2019 |
| FIX OR Shift Limited | Transport & logistics | 2026 |
| Fizzio-Fit Limited | Arts & recreation | 2006 |
| Flamstead Limited | Professional & scientific | 2015 |
| Flegg Investments 16 Limited | Real estate | 2017 |
| Flora Medical Aesthetics Ltd | Other services | 2025 |
| Florence Nails & Spa Ltd | Other services | 2025 |
| Forbo Superannuation Fund (Trustees) Limited(The) | Professional & scientific | 1986 |
| Forbo UK Limited | Professional & scientific | 1922 |
| Forbo-Nairn Limited | Manufacturing | 1931 |
| Forestworks Limited | Extraterritorial | 2020 |
| Fort Ladieswear Ltd | Wholesale & retail | 2019 |
| Fort Menswear Ltd | Wholesale & retail | 2017 |
| Fortius Fitness Ltd | Wholesale & retail | 2023 |
| FPS Fashion Ltd | Wholesale & retail | 2025 |
| Freeways Logistics Ltd | Transport & logistics | 2020 |
| Fresh AS a Daisy Cleaning Co Ltd | Administrative & support | 2023 |
| Fresh Start Professional Cleaning Services Ltd | Administrative & support | 2024 |
| Friends of Ripley Primary School Community Interest Company | Professional & scientific | 2018 |
| Frontline Leasing and Fleet Management Ltd | Information & communication | 2013 |
| Furniture Cloud Limited | Wholesale & retail | 2024 |
| Fusion Yoga Studios Ltd | Arts & recreation | 2021 |
| G Mech (Midlands) Limited | Professional & scientific | 2019 |
| G-Eight Consulting Limited | Professional & scientific | 2013 |
| G.Eden Brickwork Ltd | Construction | 2019 |
| G.H.Edwards Installations Limited | Construction | 2016 |
| G1R Drafting Services Ltd | Transport & logistics | 2019 |
| Gaskill Holdings Ltd | Financial services | 2025 |
| Gassin Holdings Ltd | Financial services | 2023 |
| Geek Logix Ltd | Information & communication | 2024 |
| GEM Equine Limited | Professional & scientific | 2009 |
| Genrx Ltd | Information & communication | 2025 |
| GHB Improvement & Coaching Specialists Ltd | Professional & scientific | 2024 |
| Gillway Douglas Holdings Ltd | Financial services | 2025 |
| GMK Compliance Services Ltd | Professional & scientific | 2025 |
| GMM Consultancy Ltd | Professional & scientific | 2026 |
| GNJ Dyson Property Ltd | Real estate | 2010 |
| Go With Nature Limited | Wholesale & retail | 2022 |
| Gold Independent Financial Services Limited | Financial services | 1998 |
| Good Times on TAP Ltd | Hospitality | 2025 |
| GOODFOODINN789 Limited | Hospitality | 2025 |
| Grace Gardens Residents Ltd | Households | 2020 |
| Graham Dickinson Taxation Services Limited | Professional & scientific | 2007 |
| Graphite Property Refurbishments Ltd | Construction | 2023 |
| Graymatter Associates Limited | Professional & scientific | 2010 |