| Elite Decoration Sussex Ltd | Construction | 2026 |
| Elite OS Scaffolding Limited | Construction | 2009 |
| Elite Scaffolding South East Ltd | Construction | 2026 |
| Elizabeth Charles Limited | Professional & scientific | 2019 |
| Ella Rock Ltd | Wholesale & retail | 2023 |
| Elliott Upton Limited | Professional & scientific | 2010 |
| Ellis Motors (Sussex) Limited | Wholesale & retail | 2017 |
| ELM Property South East Limited | Real estate | 2025 |
| ELM Tree (South East) Ltd | Extraterritorial | 2014 |
| Emerging Artists Ltd | Professional & scientific | 2019 |
| Emgp Ltd | Real estate | 2014 |
| Emjaba Ltd | Information & communication | 2017 |
| Emkay Property Ltd | Real estate | 2023 |
| Emmett & White Limited | Wholesale & retail | 2015 |
| Emtial Ltd | Professional & scientific | 2015 |
| Endo Property Ltd | Real estate | 2018 |
| Eniva Limited | Administrative & support | 2005 |
| ENT Eastbourne Limited | Professional & scientific | 2014 |
| ENT Medical Legal Limited | Professional & scientific | 2010 |
| Entrypass Limited | Information & communication | 2026 |
| EPS Lettings Ltd | Real estate | 2025 |
| Eris Hair and Beauty Limited | Extraterritorial | 2023 |
| Essential Nitrate WK Ibiza Limited | Arts & recreation | 2016 |
| Essex Community Energy CIC | Energy | 2022 |
| Estd Retail Ltd | Wholesale & retail | 2023 |
| Eternal Edge Ltd | Manufacturing | 2018 |
| Events From Within Ltd | Administrative & support | 2026 |
| Evin K Limited | Financial services | 2016 |
| Examed Limited | Professional & scientific | 2008 |
| Excaelibur Ltd | Professional & scientific | 2021 |
| Exhibitscapes Limited | Construction | 2019 |
| Express Air and Sea Services Limited | Professional & scientific | 1983 |
| Express Export Services Limited | Transport & logistics | 1974 |
| Extraordinary Expeditions Limited | Arts & recreation | 2017 |
| F R Building & Carpentry Ltd | Construction | 2023 |
| F.D.S. Consultancy Limited | Professional & scientific | 2008 |
| Fairalls (Builders Merchants) Limited | Wholesale & retail | 1989 |
| Fairalls Group Limited | Financial services | 2017 |
| Falconhurst Jevington Gardens Ltd | Households | 2021 |
| Family in Mind Kent CIC | Health & social care | 2024 |
| Fantaza ISW Limited | Health & social care | 2016 |
| Farmlands Court Residents Limited | Households | 1996 |
| FBL Design Limited | Arts & recreation | 2005 |
| Feastro Limited | Hospitality | 2017 |
| Fermenting Change CIC | Hospitality | 2025 |
| Fernau.Ch Limited | Information & communication | 2013 |
| Fernbank (Tollwood Park) RTM Company Limited | Households | 2019 |
| Ferox Construction Ltd | Construction | 2011 |
| Fictional Eyes Ltd | Information & communication | 2020 |
| Figurati Limited | Information & communication | 2017 |
| Fine Art Cards Limited | Manufacturing | 2018 |
| Fiori Consultancy London Limited | Professional & scientific | 2016 |
| Fira Software Ltd | Information & communication | 2011 |
| Fireflies Designs Limited | Wholesale & retail | 2019 |
| Fireqa Limited | Public administration | 2020 |
| Firle Bonfire Society Limited | Arts & recreation | 1997 |
| First Line Bookshop Ltd | Wholesale & retail | 2025 |
| First Serve Ltd | Hospitality | 2023 |
| Fivens Place Management Company Ltd | Administrative & support | 2015 |
| Flair Sport Academy Ltd | Education | 2018 |
| Flight ATM Systems Limited | Information & communication | 2005 |
| Flintwise Insurance Brokers Limited | Financial services | 2025 |
| Fluid Motion Limited | Administrative & support | 1991 |
| FLY Boys Investments Ltd | Real estate | 2025 |
| FML Healthcare Ltd | Health & social care | 2025 |
| Focus Coaching Limited | Professional & scientific | 2003 |
| Folkington Manor Limited | Administrative & support | 2010 |
| Fomkas Limited | Professional & scientific | 2007 |
| FOR the Forces Ltd | Wholesale & retail | 2025 |
| Foraging Foxes Ltd | Real estate | 2024 |
| Forgemoor Limited | Information & communication | 2026 |
| Formulr Limited | Professional & scientific | 2022 |
| Fortify Construction Ltd | Construction | 2024 |
| Foster Close Ltd | Households | 2009 |
| Foundations to Finished Ltd | Construction | 2022 |
| FOX Repointing and Building Maintenance Ltd | Construction | 2024 |
| Foxtest Ltd | Information & communication | 2009 |
| FPP Allison Limited | Real estate | 2026 |
| Freebird Carpentry Limited | Manufacturing | 2021 |
| Fresh Air Landscapes Ltd | Administrative & support | 2019 |
| Fresh Candy Media Limited | Professional & scientific | 2014 |
| Fresh Cleaning Services Limited | Administrative & support | 2004 |
| Friars Close Management Limited | Households | 2017 |
| Full Range Fitness Limited | Arts & recreation | 2015 |
| Fullabrook Wind Farm Community Interest Company | Other services | 2006 |
| Funky Events Ltd | Hospitality | 2024 |
| Fusion Technology Limited | Wholesale & retail | 2006 |
| Future Management & Construction (FMC) Limited | Construction | 2017 |
| Future Youth Media Ltd | Education | 2024 |
| Future-Tec Electrical Limited | Real estate | 2016 |
| Fydus IT Solutions Ltd | Information & communication | 2024 |
| G G Nicholson Ltd | Real estate | 2022 |
| G.L.A.D. + (UK) Ltd | Health & social care | 2024 |
| G.Lindsay Consultants Ltd | Construction | 2019 |
| G.M.S. Adaptations Ltd | Construction | 2011 |
| Galerie Mahout Ltd | Wholesale & retail | 2022 |
| Gamlin Holdings Limited | Financial services | 2021 |
| Garner Financial Solutions Limited | Professional & scientific | 2015 |
| Gas&Eco Plumbing&Heating Services Ltd | Administrative & support | 2016 |
| Gascomp Ltd | Administrative & support | 2016 |