| Dalane Consulting Ltd | Professional & scientific | 2011 |
| DAM Accounting Limited | Professional & scientific | 2023 |
| DAM Good Business Limited | Professional & scientific | 2023 |
| Damn Cheek Productions C.I.C. | Arts & recreation | 2016 |
| Dana Export Ltd. | Water & waste | 2009 |
| Darren Bird Construction Limited | Construction | 2007 |
| Darryl Bird Ltd | Other services | 2012 |
| Datanut Sciences (London) Limited | Professional & scientific | 2014 |
| Dathan Ltd | Administrative & support | 2020 |
| Dave Turner Financial Services Limited | Financial services | 2013 |
| Davenport Jewellers Limited | Wholesale & retail | 2026 |
| David Tovey & Virginia Morley Associates Limited | Professional & scientific | 2012 |
| DB Domestics Service & Sales Limited | Manufacturing | 2022 |
| DB Sussex Homes Ltd | Construction | 2025 |
| DE Measurement Solutions Limited | Administrative & support | 2012 |
| Deans Barn Ltd | Agriculture & fishing | 2024 |
| Deether Outsourcing Limited | Administrative & support | 2017 |
| Defence Systems Limited | Administrative & support | 2012 |
| Delta HV Ltd | Construction | 2014 |
| Demire Services Limited | Professional & scientific | 2013 |
| Demire Subscriber Limited | Extraterritorial | 2013 |
| Derdi Ltd | Hospitality | 2025 |
| Destiny Projects Ltd | Professional & scientific | 2008 |
| Devonshire Properties Limited | Real estate | 2002 |
| Devotedly Discus Limited | Wholesale & retail | 2003 |
| Dewsy Ltd | Wholesale & retail | 2015 |
| Dgag Holdings Ltd | Financial services | 2025 |
| DI Lieto's Coffee Lounge Limited | Hospitality | 2024 |
| Dibble Brothers Limited | Administrative & support | 2013 |
| Digital ROO Ltd | Professional & scientific | 2018 |
| Digitel Utilities Limited | Manufacturing | 2009 |
| Direction 7 Ltd | Information & communication | 2022 |
| Dito Ltd | Hospitality | 2023 |
| Dittons Farm Amenities Limited | Real estate | 2025 |
| Dittons Studios Limited | Professional & scientific | 1983 |
| Diverse Business Consulting Limited | Professional & scientific | 2015 |
| DJP Resin Specialists Ltd | Construction | 2023 |
| DJP SE Ltd | Construction | 2023 |
| Dlah Freehold Limited | Real estate | 2018 |
| DM Electrical and Property Services Ltd | Construction | 2025 |
| DN Engineering Ltd | Water & waste | 2020 |
| DO What Matters Ltd | Professional & scientific | 2018 |
| Document Workflow Solutions Limited | Administrative & support | 2007 |
| Docuneering Limited | Information & communication | 2017 |
| Dollys Developments Limited | Construction | 2023 |
| Domiles Holdings Ltd | Financial services | 2023 |
| Domiles Ltd | Real estate | 2023 |
| Douglas Graham Developments Limited | Construction | 1962 |
| Douglas Graham Investments Limited | Construction | 2002 |
| Downsview Holdings Limited | Construction | 2021 |
| Dream Clean Professional Limited | Administrative & support | 2024 |
| Drewek.Com Ltd | Construction | 2009 |
| Drift Services Group Ltd | Wholesale & retail | 2021 |
| Driver Training Taylormade Ltd | Other services | 2016 |
| DS & SN Properties Ltd | Real estate | 2026 |
| DS Financial Group Ltd | Financial services | 2024 |
| DT Decorators Ltd | Construction | 2011 |
| Dualcore Construction Group Ltd | Construction | 2026 |
| Dust Bunnies Forever Ltd | Administrative & support | 2022 |
| DUX Femina Films Ltd. | Information & communication | 2008 |
| Dynamic Beauty Salon Ltd | Other services | 2024 |
| Dynamic Thought Limited | Extraterritorial | 2017 |
| Dysons Heating Limited | Construction | 2013 |
| E-Centriq Ltd | Information & communication | 2004 |
| E. C. Forest Products Ltd | Wholesale & retail | 1995 |
| Earthaconter Community Interest Company | Professional & scientific | 2021 |
| Earthstone Homes Ltd | Real estate | 2026 |
| East Imperial Construction Limited | Construction | 2014 |
| East Sussex Bookkeeping Limited | Professional & scientific | 2018 |
| East Sussex Fire & Electrical Ltd | Construction | 2018 |
| East Sussex Foods Limited | Agriculture & fishing | 2018 |
| East Sussex Foster Care Association | Health & social care | 2001 |
| East Sussex PUB Company Hastings Ltd | Hospitality | 2025 |
| Eastbourne Historic Vehicle Club Limited | Other services | 2001 |
| Eastbourne Hospitality Ltd | Other services | 2019 |
| Eastbourne Ltd | Extraterritorial | 2021 |
| Eastbourne Property Shop Limited | Real estate | 2010 |
| Eastbourne Saffrons Sports Club Trust Limited | Arts & recreation | 1991 |
| Eastbourne Stage Festival Limited | Arts & recreation | 2026 |
| Ebus Solutions Ltd | Information & communication | 2021 |
| Echo ZOO Studios Ltd | Information & communication | 2018 |
| Eclipse Products and Services Ltd | Wholesale & retail | 2025 |
| Eco Amica Collective Ltd | Wholesale & retail | 2022 |
| Eco Clean Sussex Limited | Administrative & support | 2019 |
| Ecological Analytics Ltd | Professional & scientific | 2023 |
| Ecosylva Consultancy Limited | Agriculture & fishing | 2016 |
| ED Seymour Studio Ltd | Professional & scientific | 2019 |
| Edwards 798 Consultancy Ltd | Professional & scientific | 2023 |
| Egerton House Limited | Households | 1991 |
| Ehtisham Nisar Limited | Wholesale & retail | 2025 |
| EI and S Limited | Real estate | 2017 |
| Eighty Eight Wealth Management Ltd | Extraterritorial | 2022 |
| EL Quijote Spanish Club Limited | Education | 2011 |
| Elan Consultants Limited | Professional & scientific | 2012 |
| Elba Beauty Ltd | Other services | 2023 |
| Electrilon Ltd | Construction | 2024 |
| Eleven Oak Property Ltd | Real estate | 2023 |
| Eley's Estates UK Ltd | Real estate | 2026 |
| Elite Business Accounting Limited | Professional & scientific | 2016 |
| Elite Cleaning Services (Surrey) Ltd | Construction | 2006 |