| Vallivore Limited | Financial services | 2010 |
| Vanguard Fire Compliance Ltd | Construction | 2023 |
| Vape & Candy Ltd | Wholesale & retail | 2023 |
| Vasart Commercial Cleaning Limited | Administrative & support | 2015 |
| Vector Aerospace (UK Holdings) Limited | Professional & scientific | 1998 |
| Vehicle Logistics (Fife) Limited | Real estate | 2023 |
| Velocitas Performance Ltd | Information & communication | 2015 |
| Veon (Scotland) Limited | Agriculture & fishing | 2017 |
| Verimac (NO 25) Limited | Real estate | 1985 |
| Veritas Medica Ltd | Health & social care | 2024 |
| Veroken Limited | Real estate | 2020 |
| VET Your Decisions Ltd | Education | 2025 |
| Vhbr Services Limited | Other services | 2021 |
| Vickel Cleaning Services Ltd | Administrative & support | 2025 |
| Victoria Maclellan Consultancy Limited | Professional & scientific | 2024 |
| Viga Sportswear Ltd. | Manufacturing | 2012 |
| Viking Energy Partnership | | 2017 |
| Viking Energy Wind Farm LLP | | 2015 |
| Vintage Saga Ltd | Other services | 2016 |
| Virginias Furniture Hire Scotland Limited | Administrative & support | 2018 |
| Virtcareer C.I.C. | Administrative & support | 2026 |
| Virtual Reality Empathy Platform Limited | Professional & scientific | 2016 |
| Viserion Accountancy Limited | Professional & scientific | 2013 |
| Vision Bathrooms Limited | Construction | 2021 |
| Vista Travel Services Ltd | Administrative & support | 2011 |
| Vitalityme C.I.C. | Arts & recreation | 2019 |
| Vitasource Limited | Wholesale & retail | 1997 |
| Voith Turbo Limited | Manufacturing | 1962 |
| Volpa Limited | Professional & scientific | 2003 |
| Volterin Limited | Wholesale & retail | 2025 |
| Voltz Ltd | Construction | 2021 |
| Voyager Life Limited | Manufacturing | 2024 |
| Voyagercann Limited | Wholesale & retail | 2022 |
| Vsdn Properties Limited | Real estate | 2019 |
| VTH Property Ltd | Real estate | 2024 |
| Vulcan Automotive Limited | Manufacturing | 2021 |
| W a Salmond Limited | Agriculture & fishing | 2017 |
| W Dowie Fencing Ltd | Wholesale & retail | 2022 |
| W. M. Fotheringham & SON | Agriculture & fishing | 1981 |
| W.A. Mcgarrie Limited | Financial services | 2019 |
| W2 Training Limited | Education | 1999 |
| Wafb Global Energy Ltd | Wholesale & retail | 2025 |
| Walk This Way(Uk) Limited | Health & social care | 2015 |
| Walker Profiles Ltd. | Manufacturing | 1997 |
| Wallace Contracting Ltd | Construction | 2022 |
| Waqas Nawaz Ltd | Wholesale & retail | 2025 |
| Ward & Co (Perth) Ltd | Professional & scientific | 2014 |
| Washbrook Holdings Limited | Financial services | 2023 |
| Washbrook Properties Ltd | Real estate | 2025 |
| Watergate Roastery Limited | Manufacturing | 2020 |
| Watkins Films Limited | Information & communication | 2020 |
| Watson Heat Recovery Limited | Construction | 2020 |
| Watson Ventilation and Louvre Installations Limited | Construction | 2008 |
| Watsons and Kottu Limited | Hospitality | 2024 |
| Waulkmill Fishings Trustees Limited | Extraterritorial | 1990 |
| WAX Creations Limited | Financial services | 2021 |
| Wayne Robertson Properties Limited | Real estate | 2020 |
| WBS (Wester Bonhard Services) Limited | Construction | 2017 |
| WCF Enterprises C.I.C. | Manufacturing | 2021 |
| Wdjw Ltd | Other services | 2022 |
| WE ARE One Clan Ltd | Other services | 2024 |
| Webmors Coffee Shop Limited | Hospitality | 2022 |
| Wedding Solutions Awards Limited | Administrative & support | 2024 |
| Wedding Solutions Limited | Information & communication | 2020 |
| WEE India Food Bar Ltd | Hospitality | 2021 |
| WEE Scottish Rascals Limited | Hospitality | 2022 |
| WEE Three (Perth) Limited | Wholesale & retail | 2016 |
| WEE22 Management Ltd. | Professional & scientific | 2015 |
| Wegg Ltd | Professional & scientific | 2022 |
| Wego BUS Limited | Transport & logistics | 2015 |
| Well Finished Limited | Professional & scientific | 2012 |
| Well Rolled Ltd | Hospitality | 2025 |
| Wentworth Potatoes Limited | Wholesale & retail | 2026 |
| West & SON Properties Ltd | Real estate | 2025 |
| West Dron Holdings Limited | Real estate | 2021 |
| West Grange Solutions Ltd | Mining & quarrying | 2016 |
| West Third Soils Limited | Agriculture & fishing | 2025 |
| West Tulchan Limited | Agriculture & fishing | 2001 |
| Western Edge Perth Ltd | Hospitality | 2026 |
| Westhill Enterprises Limited | Administrative & support | 2017 |
| Westown Wealth Ltd | Real estate | 2022 |
| Westview Guesthouse Limited | Hospitality | 2012 |
| Westwood Farm (Scotland) Limited | Agriculture & fishing | 2008 |
| WGF Properties Ltd | Real estate | 2021 |
| Whisky Auctioneer Limited | Administrative & support | 2014 |
| White Stag Studios Ltd | Information & communication | 2025 |
| White Thistle Ltd | Hospitality | 2021 |
| Whitehill & Wilsons Limited | Extraterritorial | 1988 |
| Whitson's Ltd | Wholesale & retail | 2015 |
| Whoza AI Ltd | Information & communication | 2026 |
| Wild & Hues Limited | Wholesale & retail | 2025 |
| Wild Herb Kitchen and Catering Limited | Hospitality | 2021 |
| Wild Meds Ltd | Other services | 2017 |
| Wild Sparks Outdoor Play CIC | Education | 2014 |
| Wild Workforce CIC | Health & social care | 2025 |
| Will Mallett Ltd | Information & communication | 2024 |
| William Donaldson Limited | Wholesale & retail | 2013 |
| William Watson & Sons Limited | Real estate | 2019 |
| Wilock Perthshire Limited | Construction | 2023 |
| Wilson Construction (Perth) Limited | Construction | 2000 |