| THE Y Centre Limited | Administrative & support | 2000 |
| Thepathfinder CIC | Health & social care | 2022 |
| Thermal Comfort Limited | Construction | 2001 |
| Thirteen Real Estate Ltd | Real estate | 2025 |
| Thistle and Heart Ltd | Wholesale & retail | 2016 |
| Thistle Autoworx Limited | Wholesale & retail | 2025 |
| Thistle Stone Property Ltd | Real estate | 2024 |
| Thomas a. Sands Limited | Agriculture & fishing | 2002 |
| Thomas Jeffers Ltd | Real estate | 2024 |
| Three Little Seeds Ltd | Wholesale & retail | 2024 |
| Tiger Mouse Creative Ltd. | Administrative & support | 2013 |
| Tignano Consulting Limited | Professional & scientific | 2023 |
| Tikka N Talk Ltd | Hospitality | 2025 |
| Tillyford Company Limited | Wholesale & retail | 2025 |
| Timber Creek LOG Yards Limited | Wholesale & retail | 2017 |
| Timber Creek Stables Limited | Wholesale & retail | 2017 |
| Time Design Ltd | Other services | 2025 |
| Tinbridge Limited | Agriculture & fishing | 2010 |
| Tippermuir Books Limited | Information & communication | 2011 |
| Tiree Lettings Ltd | Hospitality | 2025 |
| TNT Ltd | Hospitality | 2026 |
| Todhill Game Ltd | Agriculture & fishing | 2023 |
| Toma Shah Ltd | Professional & scientific | 2023 |
| Tonique Ltd | Financial services | 2021 |
| Toole Electrical Contractors Ltd | Manufacturing | 2023 |
| Top Class Dejavu Limited | Wholesale & retail | 2019 |
| Top Cuts Perth Ltd | Other services | 2018 |
| Topaz Healthcare Ltd | Health & social care | 2022 |
| Torland Industries Ltd | Wholesale & retail | 2024 |
| Toroly Limited | Wholesale & retail | 2022 |
| Touch of Asia Ltd | Wholesale & retail | 2025 |
| Touch of Poland Ltd | Wholesale & retail | 2013 |
| Tourprism Limited | Information & communication | 2021 |
| Tovozo Ltd | Hospitality | 2025 |
| Town and City Parking Limited | Administrative & support | 2012 |
| TR Hunter Ltd | Real estate | 2026 |
| Trade Solutions (Energy) Limited | Construction | 2019 |
| Trade Solutions Farms Limited | Agriculture & fishing | 2023 |
| Tradebridgex UK Ltd | Information & communication | 2026 |
| Trail Architects Limited | Professional & scientific | 2017 |
| Trail Runners Bootcamp Ltd | Education | 2023 |
| Translutions Limited | Administrative & support | 2003 |
| Travelscot Limited | Administrative & support | 2022 |
| Travers Solar Company Ltd | Construction | 2025 |
| Trayndale Garden Services Ltd | Administrative & support | 2015 |
| Trayndale Services Ltd | Administrative & support | 2015 |
| Treaqua Limited | Agriculture & fishing | 2018 |
| Trees Plus Ltd. | Agriculture & fishing | 2002 |
| Trellis Scotland | Other services | 2006 |
| Trinity Community Trust | Other services | 2017 |
| Trinity Gask Eggs Limited | Manufacturing | 2025 |
| Tripsring Travels Limited | Administrative & support | 2025 |
| Tristan Petroleum Limited | Wholesale & retail | 2013 |
| Trusted Learning LLP | | 2019 |
| TSG Perth Limited | Administrative & support | 2009 |
| TTS Landscaping Ltd | Administrative & support | 2019 |
| TTS Tree Surgery Ltd | Agriculture & fishing | 2018 |
| Tully Property & Developments Ltd | Construction | 2025 |
| Tullymoran Limited | Professional & scientific | 2022 |
| Tummel Valley Leisure Limited | Agriculture & fishing | 1985 |
| Turleum Technology Limited | Information & communication | 2016 |
| Turnbull Tree Care Limited | Agriculture & fishing | 2015 |
| Turret Villa Retirement Home Limited | Hospitality | 1982 |
| TUS NUA Care Services Ltd | Health & social care | 2009 |
| Tweak Pharma Ltd | Health & social care | 2025 |
| Tweak Skin and Aesthetics Limited | Other services | 2024 |
| TYPE2ADVENTURES Ltd | Education | 2023 |
| UHE Group Limited | Financial services | 2013 |
| UIG Global Ltd | Professional & scientific | 2026 |
| UK 2MAN Limited | Transport & logistics | 2021 |
| UK Fashion and Textile Scotland Limited | Other services | 2018 |
| UK Sumon Ltd | Wholesale & retail | 2025 |
| Ulpian Systems Limited | Information & communication | 2014 |
| Ultrajoist Limited | Extraterritorial | 2002 |
| Undaunted Limited | Manufacturing | 2018 |
| Undercutters Garden and Gutters Ltd | Construction | 2025 |
| Underfloor Heating Merchants Ltd | Wholesale & retail | 2005 |
| Unguilty Beauty Ltd | Manufacturing | 2026 |
| Unite Fire Management Services Limited | Construction | 2021 |
| Unite Renewables Ltd | Energy | 2022 |
| Unite Security Electrical & Fire Ltd | Construction | 2011 |
| United Glazing Limited | Construction | 2024 |
| United Kingdom Speedrunner Gathering C.I.C. | Information & communication | 2018 |
| Unity Loft Ltd | Construction | 2025 |
| Universal Ventures Ltd | Administrative & support | 2026 |
| Unrivalled Nutrition Ltd. | Other services | 2017 |
| Until NOW Ltd | Other services | 2016 |
| Uplund Services Ltd | Manufacturing | 2025 |
| Upper Glenfintaig Estate Farm Limited | Agriculture & fishing | 2012 |
| Urquhart Developments (Scotland) Limited | Real estate | 2004 |
| Urquhart Management (Scotland) Limited | Wholesale & retail | 2004 |
| Urquhart Media Limited | Information & communication | 2016 |
| Usave Stores Ltd | Wholesale & retail | 2014 |
| Utha Hydro Ltd | Energy | 2014 |
| Utilityscout Ltd | Construction | 2025 |
| Uvdb Ltd | Agriculture & fishing | 2021 |
| VA Timber Products Ltd | Manufacturing | 2016 |
| Vagperth Ltd | Wholesale & retail | 2024 |
| Vaird Ltd | Construction | 2008 |
| Vallenus Consulting Limited | Professional & scientific | 2023 |