| THE Wild Wool Company Ltd | Wholesale & retail | 2024 |
| THE Wood Neuk Limited | Manufacturing | 2013 |
| This Way UP Global Ltd | Transport & logistics | 2015 |
| Thomson Tax Consulting Limited | Professional & scientific | 2020 |
| Thrupppence Limited | Professional & scientific | 2015 |
| Tiger Lily Gifts Ltd | Wholesale & retail | 2012 |
| Tikto LLP | | 2004 |
| Tiny Temple Ltd | Manufacturing | 2019 |
| TOM Plumb (Scotland) Ltd | Construction | 2015 |
| Topcat Inspection Ltd | Professional & scientific | 2015 |
| Totum Sports Ltd | Arts & recreation | 2025 |
| Treeline Woodlands Ltd. | Agriculture & fishing | 2004 |
| True North Architectural Design Ltd | Professional & scientific | 2026 |
| Tweed Art Ltd | Arts & recreation | 2013 |
| Tweed Bars Limited | Hospitality | 2023 |
| Tweed Ecology Ltd. | Professional & scientific | 2011 |
| Tweed Financial Services Ltd | Financial services | 2009 |
| Tweed Property Consultants Ltd | Real estate | 2020 |
| Tweed Renewables Limited | Construction | 2008 |
| Tweed Rentals Ltd | Real estate | 2025 |
| Tweed Solutions Limited | Information & communication | 2022 |
| Tweed Valley Distilling Company Ltd | Manufacturing | 2018 |
| Tweed Valley Tourism BID Limited | Other services | 2020 |
| Tweeddale Bars Ltd | Hospitality | 2014 |
| Tweeddale Bloodstock Limited | Arts & recreation | 2023 |
| Two Cooks Catering Ltd | Hospitality | 2015 |
| Untangled Web Ltd. | Information & communication | 2009 |
| Vantage Advisory Limited | Professional & scientific | 2021 |
| Vecture Ltd | Professional & scientific | 2025 |
| Ventnor FOX Ltd | Real estate | 2025 |
| Versfeld Project Delivery Limited | Information & communication | 2026 |
| VH Nails Limited | Other services | 2025 |
| Vogrie Geoservices Limited | Mining & quarrying | 2006 |
| W.G. Watson & Partners (Heathpool) | | 1981 |
| Walker Scott Ireland Ltd | Real estate | 2015 |
| Waxy Smelts Ltd | Manufacturing | 2021 |
| Wellington & Blue Ltd | Financial services | 2019 |
| West Coast Fisheries (AYR) Limited | Real estate | 1949 |
| WHC Engineering Management Ltd. | Other services | 2016 |
| Wild Bake House Ltd | Hospitality | 2021 |
| William Baillie (Peebles) Limited | Real estate | 1933 |
| Wilson Oliver Ltd | Real estate | 2025 |
| Woodvale Gallowhill Limited | Professional & scientific | 2021 |
| Woven Kinship CIC | Education | 2024 |
| Yuletech Consultancy Ltd | Information & communication | 2022 |
| Zomax Limited | | 2003 |
| Zoom Motorhome Hire Ltd | Administrative & support | 2021 |