| 13HUNDRED Creative Partners Limited | Professional & scientific | 2012 |
| 1681 North Broad ST Records Limited | Information & communication | 2020 |
| 254 Consulting Limited | Professional & scientific | 2025 |
| 36 CAP LLP | | 2023 |
| 71 Financial Management Limited | Information & communication | 2015 |
| A & C Clark Property Ltd | Real estate | 2019 |
| A & F Joinery Contractors Ltd | Construction | 2013 |
| A Macfarlane Property Investments Ltd | Real estate | 2026 |
| A.H. TOD Limited | Wholesale & retail | 1943 |
| Aberdeen Dyce Hotel Limited | Construction | 2023 |
| Aberdeen Dyce Operations Limited | Hospitality | 2023 |
| Abound Design & Interpretation Ltd | Professional & scientific | 2013 |
| Account-It Ltd | Professional & scientific | 2012 |
| Adams Napier Partnership Ltd. | Other services | 2016 |
| Adarta Limited | Information & communication | 2026 |
| ADD Accounting Solutions Limited | Professional & scientific | 2017 |
| Aeolian Grange Limited | Real estate | 2015 |
| AIM Property Co Limited | Real estate | 2019 |
| Airbrick Solutions Ltd | Construction | 2023 |
| Alastair Cunningham Limited | Administrative & support | 2001 |
| Alizan Ltd | Information & communication | 2025 |
| Alsper Consultancy Ltd | Professional & scientific | 2026 |
| Alsper Ltd | Real estate | 2020 |
| Amcows 58 Limited | Real estate | 2009 |
| Annette Ferguson Scotland Ltd | Professional & scientific | 2019 |
| Annia Ltd | Hospitality | 2023 |
| Aperspective Limited | Real estate | 2021 |
| Arkestra Ltd | Professional & scientific | 2011 |
| As-Ad Software Limited | Information & communication | 1983 |
| Atticus Interior Design Limited | Professional & scientific | 2025 |
| Bain & Irvine Ltd. | Construction | 2006 |
| Bales Capital Ltd | Real estate | 2025 |
| Barc Travel Ltd | Transport & logistics | 2017 |
| Barony Castle LLP | | 2012 |
| Basil and Fleur Ltd | Wholesale & retail | 2020 |
| Bathfizz Ltd | Wholesale & retail | 2021 |
| Beavers on Tweed Limited | Other services | 2026 |
| BEE LET Property Ltd | Real estate | 2025 |
| Beech Joinery & Building Limited | Construction | 2010 |
| Beltane Studios Limited | Manufacturing | 1996 |
| Beveridge Building & Joinery Limited | Construction | 2003 |
| Beyond Borders Productions Limited | Arts & recreation | 2010 |
| Beyond Borders Scotland Ltd | Professional & scientific | 2013 |
| Biblichor Ltd | Manufacturing | 2016 |
| Birkscairn Ltd | Real estate | 2025 |
| Black Box Group Ltd | Manufacturing | 2016 |
| Blacklodge Development Limited | Construction | 2015 |
| Blackwood & Smith LLP | | 2018 |
| Bloodline Spirits Ltd | Manufacturing | 2018 |
| Bloom Financial Planning Ltd | Professional & scientific | 2025 |
| Bonlilly Ltd | Wholesale & retail | 2025 |
| Bonnington Consulting Ltd | Information & communication | 2013 |
| Booze&Grub Ltd | Hospitality | 2025 |
| Border Architects Ltd | Professional & scientific | 2006 |
| Border Homes & Properties Limited | Real estate | 2007 |
| Borderlands Tabletop Gamers CIC | Arts & recreation | 2023 |
| Borders Chiropractic LLP | | 2010 |
| Borders Locations Ltd | Information & communication | 2026 |
| Borders LOG Cabins Limited | Hospitality | 2018 |
| Borders Mucky Paws Dog Walking Ltd | Other services | 2024 |
| Borders Online Ltd | Information & communication | 2013 |
| Borders Psychological Services Ltd | Health & social care | 2025 |
| Borders Self Catering Cabins Limited | Hospitality | 2020 |
| Brague (Peterhead) Limited | Construction | 1978 |
| Braidwood Graham Ltd. | Professional & scientific | 2016 |
| Braidwood Graham Trustees Limited | Administrative & support | 2023 |
| Branching Upwards C.I.C. | Education | 2020 |
| Brightwire Technology Services Limited | Information & communication | 2006 |
| Brown Holdings (Peebles) Limited | Other services | 2021 |
| Bruadar Properties Limited | Real estate | 2019 |
| Byprestige Limited | Professional & scientific | 2023 |
| C X W Killin Ltd | Hospitality | 2013 |
| C3 Properties (Scotland) Ltd | Real estate | 2024 |
| Cademuir Asset Management Ltd | Professional & scientific | 2024 |
| Cademuir Building Consultants Limited | Professional & scientific | 2017 |
| Cademuir Medical Ltd | Health & social care | 2024 |
| Cademuir Pharma Services Limited | Health & social care | 2015 |
| Cademuir Property (Scotland) Limited | Real estate | 2022 |
| Cairn Psychology Ltd | Health & social care | 2026 |
| Carlisle & Collins Limited | Financial services | 2002 |
| Castle Warehouse (Peebles) Ltd. (the) | Wholesale & retail | 1960 |
| Castlehill Homes Limited | Construction | 2005 |
| Castlehill Livery Limited | Administrative & support | 2009 |
| Castlehill Prop Co Limited | Real estate | 2023 |
| Caxton Contracting Ltd. | Professional & scientific | 2010 |
| Cca-Signs Ltd | Professional & scientific | 2010 |
| CCP Retail Limited | Wholesale & retail | 2023 |
| Chitendai Ltd | Professional & scientific | 2013 |
| Churros&Chocolate Limited | Hospitality | 2024 |
| Clean Slate Design Ltd | Professional & scientific | 2020 |
| Clonmel Consultancy Limited | Financial services | 2025 |
| Coates Younger Consulting Limited | Professional & scientific | 2016 |
| Cobalt Facilities Support Ltd. | Professional & scientific | 2014 |
| Cocoa Black Limited | Manufacturing | 2006 |
| Codemove Limited | Hospitality | 2001 |
| Colin Dunlop Consulting Ltd | Administrative & support | 2025 |
| Colinton Pharmacy Limited | Wholesale & retail | 1952 |
| Colour JAM Limited | Information & communication | 2025 |
| Combo Kitchen Ltd | Wholesale & retail | 2026 |
| Conservation Support Services Ltd | Professional & scientific | 2021 |