| Eldora Living Limited | Health & social care | 2025 |
| Electrical Solutions (GB) Ltd. | Construction | 2014 |
| Elegant Electronics Limited | Wholesale & retail | 1984 |
| Elegant Opolis Ltd | Wholesale & retail | 2023 |
| Elevate Events and Incentives Ltd | Hospitality | 2023 |
| Elevate Tuition Ltd | Education | 2021 |
| Elite Property Services and Security Ltd | Manufacturing | 2026 |
| Elitebright Limited | Administrative & support | 2016 |
| Elkins Development & Management Limited | Real estate | 2020 |
| Ellaaron Limited | Professional & scientific | 2012 |
| ELM Lodge Consulting Limited | Professional & scientific | 2012 |
| Elmsdene Mews Limited | Real estate | 2014 |
| Elnikety Ltd | Health & social care | 2009 |
| Eltgo Ltd | Wholesale & retail | 2023 |
| Elvidasabor Limited | Hospitality | 2025 |
| Embassy Hotel Company Limited | Administrative & support | 2009 |
| Embedded U Systems Limited | Information & communication | 2003 |
| Emblem Books Limited | Information & communication | 1981 |
| Emcasa Ltd | Hospitality | 2025 |
| Emelco Media Ltd | Information & communication | 2015 |
| Emgate Assets Limited | Real estate | 2022 |
| Emily Jane Communications Ltd | Professional & scientific | 2018 |
| Emiscient Limited | Information & communication | 2006 |
| Empire Estates (UK) Limited | Real estate | 1996 |
| Empowering Education Tutoring and Training Ltd | Education | 2025 |
| EMS3 Ltd | Professional & scientific | 2013 |
| ENC Build Limited | Construction | 2026 |
| Endymion Ltd | Real estate | 2018 |
| Enerco Global Limited | Wholesale & retail | 2013 |
| Enervision Limited | Mining & quarrying | 2021 |
| Engsv Limited | Wholesale & retail | 2025 |
| Enhanced Body Clinic Limited | Other services | 2018 |
| Enodoc Properties Limited | Real estate | 2012 |
| Enofait Consultancy Ltd | Information & communication | 2009 |
| Ensemble HRD Ltd | Professional & scientific | 2017 |
| Enterprise Staffing and Consulting Limited | Information & communication | 2025 |
| Entrepreneurs Inspiration Ltd | Financial services | 2024 |
| Eolas Properties Limited | Real estate | 2022 |
| EOP Audio Limited | Professional & scientific | 2023 |
| Eparu Limited | Health & social care | 2018 |
| Epcom Limited | Administrative & support | 2008 |
| EPG 3 Ltd | Professional & scientific | 2016 |
| Epitome HR Limited | Professional & scientific | 2020 |
| Eposcentral Limited | Information & communication | 2012 |
| Equip and Supply Ltd | Wholesale & retail | 2026 |
| Equitopia Ltd | Other services | 2014 |
| Equitopia Stud Ltd | Agriculture & fishing | 2020 |
| Erikaotero Ltd | Other services | 2025 |
| Eros Carriage Limited | Transport & logistics | 2010 |
| Esaa Investments Ltd | Real estate | 2020 |
| ESS Eyecare Ltd | Health & social care | 2016 |
| Essam Properties Limited | Real estate | 2019 |
| Essence PR Limited | Professional & scientific | 2012 |
| Esskesh Limited | Professional & scientific | 2021 |
| Estel Engineering UK Limited | Other services | 2020 |
| Etek Retail Solutions UK Ltd | Wholesale & retail | 2018 |
| Ethan B & B Limited | Hospitality | 2016 |
| Ethereality Ltd | Professional & scientific | 2015 |
| Ethycalm Limited | Wholesale & retail | 2017 |
| Etica Limited | Other services | 2022 |
| Eton Care Ltd | Health & social care | 2015 |
| Etonwall Limited | Real estate | 2019 |
| Euro Steel Holdings Ltd | Extraterritorial | 2006 |
| Eurode Consulting Ltd | Professional & scientific | 2026 |
| Eurogate Estates UK Limited | Financial services | 2024 |
| Euroscene Communications Limited | Information & communication | 1996 |
| Everyday Value Ltd | Wholesale & retail | 2026 |
| Evolve Investment Services Limited | Households | 2023 |
| EWM Design & Build Ltd | Construction | 2024 |
| Excel Pharma Limited | Wholesale & retail | 2009 |
| Excell Associates Limited | Information & communication | 2009 |
| Exclusively People Ltd | Professional & scientific | 2021 |
| Exhibition Lifesciences Ltd | Professional & scientific | 2019 |
| Eximius Live-In Care Limited | Health & social care | 2014 |
| Exomids LP | | 2016 |
| Expansion Solutions Ltd | Professional & scientific | 2020 |
| Expert AC Ltd | Construction | 2024 |
| Expert Appliance Repairs Ltd | Other services | 2023 |
| Express Mobile Phones Ltd | Wholesale & retail | 2023 |
| Express Mobile Sales Ltd | Wholesale & retail | 2024 |
| Express Transport (UK) Ltd | Transport & logistics | 2011 |
| EYE Design Opticians Limited | Wholesale & retail | 2006 |
| EYE Excellence Limited | Health & social care | 2011 |
| Eyes in Motion Ltd | Wholesale & retail | 2018 |
| Eyex Properties Limited | Real estate | 2024 |
| Eyre Park Limited | Professional & scientific | 2010 |
| F & F Property Partners (Bury ST Edmunds) Limited | Real estate | 2025 |
| F P Accountancy Services Limited | Professional & scientific | 2005 |
| F. Nicolas Franchising Limited | Other services | 2010 |
| Facilis Business Limited | Professional & scientific | 2022 |
| Fairfax Solutions Limited | Information & communication | 2013 |
| Falconz Consultancy Ltd | Professional & scientific | 2026 |
| Famchat Ltd | Real estate | 2019 |
| Famelodge Limited | Hospitality | 1994 |
| Faremine Limited | Administrative & support | 2005 |
| Farr & Farr Estate Agents (Brockworth) Limited | Real estate | 2013 |
| Fashion for Cause | Health & social care | 2018 |
| Fast and Easy Learning Limited | Education | 2023 |
| Fastbiz Ltd | Hospitality | 2023 |
| FAT Moose Media Ltd | Information & communication | 2023 |