| Eppleton Farm | Agriculture & fishing | 2012 |
| Equalactual Limited | Professional & scientific | 1987 |
| EQUI-K9 Ltd | Professional & scientific | 2021 |
| Equinox Commercial Finance Limited | Professional & scientific | 2019 |
| Equinox Contracts (North-East) Limited | Construction | 2025 |
| Essential Business Publications Limited | Information & communication | 2014 |
| Ethan Hammerton Racing Ltd | Information & communication | 2019 |
| ETP Consulting Limited | Professional & scientific | 2010 |
| European Turfgrass Specialists Limited | Professional & scientific | 2010 |
| Everest Fundraising Ltd | Professional & scientific | 2025 |
| Evolving Perspectives Limited | Professional & scientific | 2017 |
| Exelby Services Limited | Wholesale & retail | 1976 |
| Express Consulting Ltd | Professional & scientific | 2021 |
| Eyles Enterprises Limited | Real estate | 2014 |
| Face to Face Research Limited | Other services | 2002 |
| Factual Productions Limited | Information & communication | 2003 |
| Fairholme Farming Limited | Agriculture & fishing | 2012 |
| Fanchants.Com Ltd | Information & communication | 2006 |
| FAR END Holdings Limited | Financial services | 2025 |
| FAR END Properties Limited | Financial services | 2025 |
| FAT Choy 168 Ltd | Hospitality | 2019 |
| Fencote Holdings Limited | Real estate | 2025 |
| Fenetek Ltd | Manufacturing | 2014 |
| Fenion & Rainbow Properties Ltd | Real estate | 2023 |
| Fertility Retreats Ltd | Health & social care | 2020 |
| Fewbits Ltd | Wholesale & retail | 2024 |
| Fibre Vault Limited | Construction | 2023 |
| Fidence Consulting Limited | Professional & scientific | 2020 |
| Field CPS Ltd | Wholesale & retail | 2017 |
| Finehome Developments Limited | Professional & scientific | 1965 |
| Finsys Limited | Other services | 2008 |
| FIR Lodge Court Management Limited | Hospitality | 1988 |
| Firby Propco Limited | Real estate | 2020 |
| First North Law Limited | Information & communication | 2016 |
| First Project Management and Consultancy Ltd | Professional & scientific | 2022 |
| First Shout Fire Safety Management Limited | Public administration | 2023 |
| Firth and Co Wine Merchants Limited | Wholesale & retail | 2009 |
| Fisher Developments Limited | Real estate | 2023 |
| Fishtins Limited | Wholesale & retail | 2024 |
| Five Daughters Unlimited | Financial services | 2021 |
| Flareguide Fire Limited | Manufacturing | 1997 |
| Flareguide Fire Safety Limited | Professional & scientific | 2012 |
| Flawless &Confident Enterprise Ltd | Wholesale & retail | 2020 |
| FLO Investment Properties Limited | Real estate | 2019 |
| Flophie Property Ltd | Real estate | 2022 |
| Flotmanby Wold Farming | Administrative & support | 2011 |
| Flow Wellbeing Ltd | Health & social care | 2024 |
| Forcett Hall Estate Limited | Real estate | 2023 |
| Fordy Farms (Ingleby) Limited | Agriculture & fishing | 1973 |
| Fordy Thompson (Holdings) Limited | Construction | 2014 |
| Fordy Thompson Holdings EOT Trustee Limited | Construction | 2026 |
| Forem (Properties) Ltd. | Real estate | 1992 |
| Forest B.P. LLP | | 2004 |
| Forest Health Ltd | Health & social care | 2026 |
| Formula Alloys Limited | Manufacturing | 2024 |
| Forward Zimbabwe Ltd | Wholesale & retail | 2023 |
| Foster's Decorators (North East) Ltd | Construction | 2011 |
| Fraser Plahe Limited | Health & social care | 2010 |
| Friston Estates Ltd | Professional & scientific | 2015 |
| Froneri Ice Cream UK Limited | Manufacturing | 1967 |
| Froneri International Limited | Manufacturing | 2006 |
| Froneri Limited | Financial services | 2016 |
| Froneri South Africa Holdings Limited | Financial services | 2015 |
| Froneri UK Finco (NZD) Limited | Financial services | 2020 |
| Froneri UK Finco Limited | Financial services | 2020 |
| Fronte.Io Ltd | Information & communication | 2021 |
| Fusion CSE Limited | Administrative & support | 2013 |
| Fuze Applications Limited | Information & communication | 2022 |
| G Abbott & Sons Holdings Limited | Extraterritorial | 2015 |
| G Abbott & Sons Limited | Transport & logistics | 2015 |
| G R Ductwork Installation Ltd | Construction | 2003 |
| G R P Excavations Limited | Construction | 2004 |
| G Snowball & SON Limited | Construction | 2003 |
| G W Thompson & SON Limited | Professional & scientific | 2026 |
| G&T Aquatics Limited | Construction | 2020 |
| G.A.M. Consulting Limited | Professional & scientific | 2014 |
| G.A.Turnbull Limited | Agriculture & fishing | 2014 |
| G.T. Hutton (Flowers) Limited | Wholesale & retail | 1981 |
| Gadred Property Ltd | Real estate | 2025 |
| GAM Nominees AEM Limited | Financial services | 2022 |
| GAM Nominees GCV Limited | Financial services | 2023 |
| GAM Nominees Limited | Financial services | 2022 |
| GAM Nominees LIS Limited | Financial services | 2024 |
| GAM Nominees NLI Limited | Financial services | 2022 |
| GAM Nominees TNO Limited | Financial services | 2023 |
| GAM Nominees VVI Limited | Financial services | 2022 |
| Gaspara Asset Management LLP | | 2020 |
| Gaspara Orange LLP | | 2020 |
| Gaspara Yellow LLP | | 2021 |
| Gavin Horsman Logistics Ltd | Transport & logistics | 2024 |
| GB Furnishings Ltd | Wholesale & retail | 2017 |
| GB Railway Data Ltd | Information & communication | 2022 |
| GCM4 Limited | Administrative & support | 2019 |
| GE Media Limited | Information & communication | 2012 |
| Gemma Tutty Racing Ltd | Arts & recreation | 2025 |
| Genesis Healthcare Recruitment Ltd | Administrative & support | 2021 |
| Gentle HEARTS24 Ltd | Administrative & support | 2024 |
| Gentlelay Limited | Manufacturing | 2022 |
| George Craven Limited | Hospitality | 2005 |
| George Fordy Properties (Holdings) Limited | Real estate | 2014 |