| 1 Atmosphere Ltd | Wholesale & retail | 2023 |
| 12 Drams Limited | Wholesale & retail | 2025 |
| 1457 Golf Limited | Arts & recreation | 2021 |
| 1879 Events Limited | Professional & scientific | 2011 |
| 40 ACRES2 Limited | Construction | 2023 |
| 555 Contracts Limited | Agriculture & fishing | 2021 |
| 66M Ltd | Professional & scientific | 2024 |
| 76R Limited | Arts & recreation | 2024 |
| A&M Management Group Ltd | Real estate | 2025 |
| A. & J. Mcdowall Limited | Agriculture & fishing | 1958 |
| Actm Holdings Limited | Professional & scientific | 2017 |
| Adamson Violins Ltd | Professional & scientific | 2015 |
| Aford Property Ltd | Real estate | 2019 |
| Agentive Coaching Ltd | Professional & scientific | 2025 |
| Agron-Led Ltd | Manufacturing | 2014 |
| Aisling Callaghan Consulting Limited | Professional & scientific | 2015 |
| AJ Mitchell Limited | Manufacturing | 2015 |
| Alba Game Fishing Limited | Administrative & support | 2004 |
| ALL About Countrywear Limited | Wholesale & retail | 2019 |
| Alon Associates Ltd | Professional & scientific | 2025 |
| Anderson Saltire Property Limited | Real estate | 2012 |
| Andstrat (NO. 420) G Limited | Real estate | 2017 |
| Apopo AI Limited | Information & communication | 2026 |
| Arbat Partners Ltd | Information & communication | 2026 |
| Archerfield Wildlife Centre Limited | Professional & scientific | 2003 |
| Archie Lyle Ltd | Manufacturing | 2025 |
| Architectural Colour Coatings Ltd | Construction | 2006 |
| Ardmoor Limited | Wholesale & retail | 2010 |
| Ardtaraig Farming Company Limited | Real estate | 2019 |
| Aris Collective Ltd | Information & communication | 2025 |
| Askernish Consultancy Limited | Administrative & support | 2012 |
| Astral Nests Ltd | Wholesale & retail | 2025 |
| Atelier Vander Pollen Limited | Professional & scientific | 2026 |
| Atlantas Security & Electrical Limited | Construction | 2005 |
| Audrey Nightingale Limited | Information & communication | 2018 |
| Babspecs Limited | Real estate | 2019 |
| Ballanby Limited | Manufacturing | 2014 |
| Bandeath Holdings Limited | Real estate | 2018 |
| Barbour Marine Ltd | Manufacturing | 2023 |
| Barker & Bones Ltd | Wholesale & retail | 2019 |
| Bass Rock Financial Management Limited | Professional & scientific | 2021 |
| Bass Rock Scaffolding Ltd | Construction | 2022 |
| Because WE KAN Ltd | Professional & scientific | 2019 |
| Beck Executive Travel Ltd | Transport & logistics | 2022 |
| Bennet by Design Limited | Construction | 2017 |
| Bethog Ltd | Information & communication | 2024 |
| Binning Memorial Wood Limited | Other services | 2009 |
| Biometis Consulting Limited | Professional & scientific | 2018 |
| Black Sugar Music Limited | Information & communication | 2010 |
| Blue Wild Limited | Transport & logistics | 2016 |
| Blues & Greens Limited | Wholesale & retail | 2007 |
| Bolton Muir Studios Limited | Real estate | 2025 |
| Borzel Ltd. | Transport & logistics | 2012 |
| Bostock Bakery Ltd | Hospitality | 2020 |
| Boundless Hospitality Ltd | Wholesale & retail | 2021 |
| Brae U.K. Ltd | Real estate | 2025 |
| Braiff Ltd | Administrative & support | 2023 |
| Bravo Whisky Golf Ltd | Administrative & support | 2018 |
| Breaking Strain Events Limited | Administrative & support | 2013 |
| Breaking Strain Ltd | Information & communication | 2021 |
| Buckit Ltd | Information & communication | 2022 |
| C J W R Properties Limited | Real estate | 2019 |
| C&J Wright Consulting Ltd | Professional & scientific | 2022 |
| Calella 98 Ltd | Wholesale & retail | 2021 |
| Cambrolyst Limited | Professional & scientific | 2022 |
| Carluke Commercial LLP | | 2019 |
| Caro NB Ltd | Wholesale & retail | 2021 |
| Catalyst Print Solutions Limited | Professional & scientific | 2005 |
| Chalmers-Watson Ardtaraig Ltd. | Financial services | 2019 |
| Changing Faces Weddings Ltd | Administrative & support | 2026 |
| Charles Blair Properties Ltd | Extraterritorial | 2024 |
| Charles Blair Sales Ltd. | Construction | 2008 |
| Charley Services Limited | Professional & scientific | 2018 |
| Cherrylkb Limited | Education | 2023 |
| Cherrytrees Childrens Nurseries Limited | Education | 2000 |
| Ciadana Healthcare Limited | Professional & scientific | 2018 |
| Civs 2014 Hospitality Ltd | Hospitality | 2018 |
| Clean Ocean Festival Ltd | Arts & recreation | 2019 |
| Clear Content Collective Ltd | Professional & scientific | 2026 |
| Clingfish Ltd | Information & communication | 2020 |
| Clock House Workshop Ltd | Real estate | 2023 |
| Clockhouse Furniture Limited | Manufacturing | 2005 |
| Cloud Farming Limited | Agriculture & fishing | 2017 |
| Clova Lettings Limited | Real estate | 2024 |
| Cnp-Mara Limited | Wholesale & retail | 2012 |
| Coastal Housekeeping East Lothian Ltd | Administrative & support | 2025 |
| COD Design Limited | Wholesale & retail | 2016 |
| Connect Links Consulting Ltd | Professional & scientific | 2019 |
| Consultancy Growth Enablement Ltd | Professional & scientific | 2026 |
| Consultancy Growth Ltd | Professional & scientific | 2024 |
| Contagion Controls Ltd | Manufacturing | 2020 |
| Contemporary Club Leadership Ltd | Professional & scientific | 2017 |
| Contemporary Club Management Ltd | Professional & scientific | 2017 |
| Cooleens Limited | Professional & scientific | 2011 |
| Core Business Psychology Limited | Professional & scientific | 2010 |
| Craigentoul Limited | Other services | 2019 |
| Craigleith Investments Limited | Real estate | 2009 |
| Creem Ventures Limited | Hospitality | 2015 |
| CSB Plumbing Heating and GAS Ltd | Construction | 2025 |
| CW Recruitment Specialists Ltd | Administrative & support | 2012 |