| D. Fairnie and Sons Limited | Agriculture & fishing | 1987 |
| D.C. Watson & Sons (Fenton Barns) Limited | Agriculture & fishing | 1929 |
| Darcon Steel Fitters Ltd | Construction | 2024 |
| DATAM8 Ltd | Information & communication | 2024 |
| Davaar Whisky Ltd | Wholesale & retail | 2023 |
| David Robinson Design Ltd. | Manufacturing | 2002 |
| Dbsa Ltd | Wholesale & retail | 2007 |
| Decorating Solutions (Scotland) Ltd | Construction | 2020 |
| Deepsea Construction Services Ltd | Construction | 2022 |
| Dementia Friendly East Lothian Community Interest Company | Health & social care | 2016 |
| Dere Street Ltd | Agriculture & fishing | 1959 |
| Dolphin Education Ltd | Education | 2024 |
| Donaldson Education Services Ltd | Education | 2014 |
| Douglas-Hamilton Management | Professional & scientific | 2008 |
| DR Colin Stirrat Limited | Health & social care | 2020 |
| Drawing Room Interior Design Studio Limited | Professional & scientific | 2025 |
| Drem Productions Ltd | Arts & recreation | 2025 |
| Drink Tech Limited | Information & communication | 2017 |
| Drive Event Solutions Limited | Education | 2009 |
| DSH Contracts Ltd | Construction | 2023 |
| Dunbar Plant Limited | Administrative & support | 2011 |
| Dunbar Removals and Storage Ltd. | Transport & logistics | 1996 |
| Dynamic LED Ltd | Professional & scientific | 2010 |
| Earth Squared Limited | Wholesale & retail | 2008 |
| East & West Advisory Ltd | Professional & scientific | 2019 |
| East Coast Assets Ltd | Real estate | 2019 |
| East Coast Competitions Ltd | Arts & recreation | 2025 |
| East Coast Lobsters and Seafood Ltd | Hospitality | 2020 |
| East Linton Saleground Limited | Real estate | 2015 |
| East Lothian Beauty Ltd | Other services | 2022 |
| East Lothian Self Defence Ltd | Arts & recreation | 2026 |
| Ecottrell Ltd | Professional & scientific | 2023 |
| Edinburgh SC (Self Catering) Limited | Real estate | 2006 |
| Edinburgh Structural & RIP OUT Ltd | Construction | 2026 |
| EFD Projects Ltd | Manufacturing | 2025 |
| EG Chef Consultant Ltd | Hospitality | 2020 |
| Elevate Sports Group Ltd | Education | 2025 |
| ELF Project Services Limited | Information & communication | 2026 |
| Emma Cross the Store Ltd | Wholesale & retail | 2023 |
| Ensign Arena Letting Company Limited | Real estate | 2024 |
| Epic Solutions Limited | Information & communication | 2016 |
| Eputar Ltd | Information & communication | 2015 |
| Explorus Ltd | Professional & scientific | 2018 |
| Exposure Scotland Ltd | Professional & scientific | 2010 |
| Eyra Clothing Ltd | Wholesale & retail | 2021 |
| Ezee Riders Ltd. | Administrative & support | 2020 |
| Factum Logistics Ltd | Transport & logistics | 2023 |
| Fairground Communications Ltd | Professional & scientific | 2015 |
| Fairnielaw Ltd | Financial services | 2003 |
| Falling Into Place Ltd | Information & communication | 2022 |
| FBC Fitness Ltd | Education | 2015 |
| Fenton Angling & Fisheries Ltd | Agriculture & fishing | 2023 |
| Fenton Barns Nursery Ltd | Health & social care | 2011 |
| Fenton Brunt Club Limited | Arts & recreation | 2017 |
| Fenton Tower Limited | Hospitality | 1999 |
| Ferrygate Developments Limited | Real estate | 2004 |
| Ferrygate Farm Fresh Eggs LP | | 2010 |
| Fidra Consultants Limited | Professional & scientific | 2025 |
| Fidra Consulting Ltd | Information & communication | 2010 |
| Fidra Fine Art Limited | Wholesale & retail | 2012 |
| Fidra Wealth Management Limited | Financial services | 2009 |
| Fidrasoft Limited | Information & communication | 2017 |
| Field and Thorn Woodcraft Co Ltd | Manufacturing | 2025 |
| Finbo Adventure Ltd | Arts & recreation | 2020 |
| Finbo Ltd | Real estate | 2019 |
| Findlay Access Services Ltd. | Mining & quarrying | 2003 |
| Fire Finch Press Ltd | Information & communication | 2024 |
| Firemac FM Blue Limited | Wholesale & retail | 2013 |
| Firemac Holdings Limited | Financial services | 2021 |
| FJH Global Ltd | Professional & scientific | 2018 |
| Flatbush Ltd | Arts & recreation | 2023 |
| Flavpharma Ltd | Manufacturing | 2023 |
| Foreways Developments Ltd | Transport & logistics | 2018 |
| Forth Land Care Ltd | Water & waste | 2007 |
| Forth Marine Resources Ltd | Agriculture & fishing | 2018 |
| Forth Resource Management Limited | Water & waste | 2002 |
| Foster Safety Solutions Limited | Administrative & support | 2013 |
| Fraser Project Management Ltd | Information & communication | 2024 |
| Fraser Thomson Consultancy Ltd | Professional & scientific | 2022 |
| Freumh Limited | Information & communication | 2024 |
| Fringe by the Sea Limited | Arts & recreation | 2008 |
| FT Project Consultants Ltd | Construction | 2025 |
| Genesis Savoir Ltd | Wholesale & retail | 2022 |
| GEO Certification | Professional & scientific | 2015 |
| GEO Foundation for Sustainable Golf Ltd | Professional & scientific | 2005 |
| GF Golf Limited | Arts & recreation | 2016 |
| Gilhooley Project Management Ltd | Professional & scientific | 2018 |
| Gillanders Psychology Services Ltd | Professional & scientific | 2017 |
| Gilmerton Land Services Ltd | Water & waste | 2015 |
| Gilsland Park Ltd | Hospitality | 2024 |
| GJ Premier Projects Limited | Construction | 2026 |
| GL Capital Ltd. | Financial services | 2012 |
| GL Langbury Limited | Hospitality | 2017 |
| Glen Lyon Limited | Mining & quarrying | 2025 |
| Glenco Investments Limited | Extraterritorial | 2004 |
| Glenwood Commerce (UK) Limited | Wholesale & retail | 2014 |
| Glow Wild Ltd | Health & social care | 2025 |
| GO4BETTER Ltd | Hospitality | 2019 |
| Goldies Mobile Tyres Limited | Manufacturing | 2025 |
| Golf Finance Limited | Financial services | 2000 |