| Ploughing Championships (Scotland) Limited | Other services | 2010 |
| Port Castle Limited | Agriculture & fishing | 1960 |
| Port William Community Development Trust | Other services | 2006 |
| Port William Inshore Rescue Service | Other services | 1998 |
| Pouton Limited | Agriculture & fishing | 2020 |
| Praiseworthy Exhibitions Limited | Administrative & support | 2025 |
| Pularyan Burn Hydro Limited | Energy | 2012 |
| Pultadie Farmers | | 1997 |
| Quarter Farmers | | 1980 |
| Quirky Quines Ltd | Wholesale & retail | 2015 |
| R&B Groundworks Limited | Construction | 2017 |
| R. and E. Vance Limited | Agriculture & fishing | 1987 |
| R.E Future Ltd | Financial services | 2022 |
| R.G. Contracting Limited | Administrative & support | 2003 |
| Rebyl Vybes Limited | Wholesale & retail | 2021 |
| Redburn House Limited | Hospitality | 2001 |
| Rh&D Farm Controls Limited | Agriculture & fishing | 2025 |
| Riverwood House Ltd | Real estate | 2023 |
| Robinson-Wyllie Limited | Agriculture & fishing | 1995 |
| Rory Crosbie Property Ltd | Real estate | 2021 |
| Rowan Glen Limited | Manufacturing | 2022 |
| Saint Ninian's Trading Limited | Wholesale & retail | 2015 |
| SDS Smart Digital Solution LP | | 2017 |
| Shennanton Livestock Limited | Agriculture & fishing | 2023 |
| Shire Agri Limited | Administrative & support | 2015 |
| Shrewd Foodie Ltd | Professional & scientific | 2022 |
| Simpson & Carnochan Limited | Professional & scientific | 2017 |
| Snap Solutions Ltd | Professional & scientific | 2024 |
| South West Recoveries Limited | Transport & logistics | 1982 |
| Sprinkler Electrical Services Ltd | Construction | 2024 |
| ST Medical Solutions LP | | 2017 |
| Starland Plus LP | | 2016 |
| Stewartry Tyres (Newton Stewart) Limited | Wholesale & retail | 2013 |
| SW Communications Limited | Information & communication | 2020 |
| SWS Mobile Tyres Ltd | Manufacturing | 2025 |
| Symplex Trade LP | | 2011 |
| Tangletastic Ltd | Wholesale & retail | 2025 |
| Tareo Commerce LP | | 2016 |
| TCI Engineering Ltd | Professional & scientific | 2019 |
| THE Barquhill Farming Company | | 1992 |
| THE Breakpad Limited | Administrative & support | 2012 |
| THE Decorators DEN Ltd | Information & communication | 2025 |
| THE Hoof GP Ltd | Agriculture & fishing | 2021 |
| THE Items to Hours Project Limited | Water & waste | 2026 |
| THE Mediterranean Touch Limited | Wholesale & retail | 2006 |
| THE MID Galloway Community Day Centre for Older People at Newton Stewart | Health & social care | 2003 |
| THE Nineteenth Hole Ltd | Hospitality | 2025 |
| THE Rolling Dough Ltd | Hospitality | 2023 |
| THE Scottish Vintage Tractor and Engine Club | Arts & recreation | 1997 |
| THE Skeleton Fund CIC | Manufacturing | 2022 |
| THE South West of Scotland Horse Driving Club Limited | Arts & recreation | 1997 |
| THE View (Pirsac) Ltd | Hospitality | 2020 |
| THE Whithorn Trust | Arts & recreation | 1988 |
| THE Wigtown Ploughman Limited | Hospitality | 2024 |
| TIM J. Mcgarva | | 1994 |
| Tinne Beag Workers Co-Operative Limited | Agriculture & fishing | 1999 |
| Titon Limited | Hospitality | 2022 |
| Trendcom Solutions LP | | 2017 |
| Trustinvest Capital LP | | 2012 |
| Tutto QUI Limited | Hospitality | 2014 |
| Unicorn Goldman LLP | | 2014 |
| Unity Corporate Services Ltd | Professional & scientific | 2018 |
| Unity Financial Consultants LLP | | 2016 |
| US Historic Newspapers Archives Limited | Wholesale & retail | 1996 |
| Void Studios Ltd | Arts & recreation | 2021 |
| W.O.B. Trading LP | | 2022 |
| Wadsworth Farming Ltd | Agriculture & fishing | 2023 |
| WBS Global Trading LP | | 2016 |
| Wellaut Trading LP | | 2016 |
| West Kirkland Farm Limited | Agriculture & fishing | 2008 |
| West View (Monreith) Limited | Health & social care | 1997 |
| Whiskybroker Limited | Wholesale & retail | 2013 |
| Whyte’S Property Management Ltd | Hospitality | 2018 |
| Wigtown & Bladnoch Community Initiative | Other services | 2017 |
| Wigtown Agricultural Society | Other services | 2010 |
| Wigtown Brewery Ltd | Manufacturing | 2019 |
| Wigtown Festival Company | Arts & recreation | 2007 |
| Wigtownshire Properties Ltd | Real estate | 2025 |
| Wigtownshire Property Management Ltd | Administrative & support | 2023 |
| Wiseman Sohal Developments Ltd | Construction | 2024 |
| WJM Tech Limited | Other services | 2014 |
| ZIG LAB LP | | 2022 |