| Galloway Stone Products Ltd | Manufacturing | 2012 |
| Gameric LP | | 2021 |
| GAW Transport Limited | Transport & logistics | 2026 |
| GLA Jeweller Ltd | Wholesale & retail | 2024 |
| Gleniron Farmers | | 1980 |
| Glenluce Computers Ltd | Other services | 2018 |
| Glenluce GUN Room Limited | Wholesale & retail | 2011 |
| Glenquicken ENG. Ltd | Energy | 2016 |
| Glentrool & Bargrennan Community Trust | Administrative & support | 2010 |
| Glenure Farm Partnership | | 1981 |
| Global JET Service LP | | 2022 |
| H J Walker & Company Limited | Agriculture & fishing | 2008 |
| H Thomson & SON Limited | Transport & logistics | 2006 |
| Hamza Superstore Ltd | Wholesale & retail | 2024 |
| Heron Drainage Services Limited | Water & waste | 2012 |
| High Eldrig Farm | | 2003 |
| High Milton Farm Ltd | Agriculture & fishing | 2014 |
| High Milton Green Energy Ltd. | Energy | 2011 |
| HORSES4HELP Community Interest Company | Health & social care | 2015 |
| I W Taylor Limited | Construction | 2017 |
| IAN Chambers Ltd | Professional & scientific | 2024 |
| Inspiration in Print Limited | Professional & scientific | 2009 |
| J & T Mitchell Limited | Wholesale & retail | 2013 |
| J Mitchell Fishing Limited | Agriculture & fishing | 2013 |
| J Renwick Consulting Ltd | Wholesale & retail | 2020 |
| J&B Print Ltd | Manufacturing | 2009 |
| J&J Mckeown Ltd | Manufacturing | 2013 |
| J. D. Owen & SON (Butchers) Limited | Wholesale & retail | 2003 |
| Jamie Hyslop Limited | Construction | 2014 |
| JAS & Keshi Ltd | Hospitality | 2024 |
| JC and Sons Transport Ltd | Transport & logistics | 2023 |
| Jmgaqua Ltd | Other services | 2014 |
| K & I Cameron Limited | Agriculture & fishing | 2025 |
| K J Jointing Ltd | Construction | 2024 |
| Keith & Fiona Mcelrea Limited | Wholesale & retail | 2008 |
| Kids Healthcare Ltd | Wholesale & retail | 2021 |
| Kilgallioch Community Benefit Company Ltd. | Administrative & support | 2017 |
| Kiltersan Farm | | 1981 |
| Kinnear for Carpets Limited | Wholesale & retail | 2015 |
| Kirkcowan Community Development Trust | Other services | 2015 |
| Kirkcowan Viking Limited | Construction | 2006 |
| Kirkmoor.Com Limited | Information & communication | 2002 |
| Kirvennie Limited | Agriculture & fishing | 2026 |
| Kitchen Coos & Ewes Ltd | Education | 2019 |
| Kufara Properties Ltd | Real estate | 2019 |
| Kumar in Style Ltd | Hospitality | 2026 |
| Kurdish Barber NTS Ltd | Other services | 2024 |
| Ladder Limb Limited | Manufacturing | 2010 |
| Landos Lobster POT Ltd | Agriculture & fishing | 2020 |
| Larg Farming Company Limited | Agriculture & fishing | 2003 |
| Latha ÙR Ltd | Manufacturing | 2023 |
| LBA Property Rentals Ltd | Hospitality | 2021 |
| Lindsay Construction Limited | Construction | 2003 |
| Line and Latitude Ltd | Financial services | 2026 |
| Luce Arts Community Interest Company | Arts & recreation | 2026 |
| Lucky 398 Limited | Hospitality | 2025 |
| Machars Action Ltd. | Administrative & support | 1995 |
| Machars Car Club Limited | Arts & recreation | 1977 |
| Machars Ecology Ltd. | Professional & scientific | 2010 |
| Machars Veterinary Services Ltd | Professional & scientific | 2019 |
| Made by Taylor Ltd | Real estate | 2019 |
| Magicom LP | | 2016 |
| Make IT SO Enterprises Ltd | Manufacturing | 2025 |
| Matthews Legal Holdings Limited | Financial services | 2025 |
| Matthews Legal Limited | Professional & scientific | 2015 |
| Matthews Nominees Limited | Professional & scientific | 2015 |
| Mcallister Marine Services Limited | Agriculture & fishing | 2001 |
| Mccabe Excavations Ltd | Construction | 2025 |
| Mcculloch Stores Limited | Wholesale & retail | 2022 |
| Mcknight Rural Consultancy Ltd | Professional & scientific | 2025 |
| Mcneill Estates | Agriculture & fishing | 1947 |
| MCO Technical Ltd | Professional & scientific | 2021 |
| Mdk-Analytics.Co.Uk Limited | Information & communication | 2026 |
| Millar Farm Ltd | Agriculture & fishing | 2021 |
| Millar Resort Ltd | Hospitality | 2022 |
| Mind the GAP Research and Training, Scotland Limited | Professional & scientific | 2023 |
| Mindcoach Developments Limited | Professional & scientific | 2015 |
| Minnigaff Community Nursery | Education | 2011 |
| Mocoram Limited | Hospitality | 2025 |
| Monreith Arms Ltd | Construction | 2020 |
| Morgtech Ltd | Professional & scientific | 2022 |
| MQ Renovations Ltd | Professional & scientific | 2025 |
| MW Engineering & Fabrication Ltd | Other services | 2016 |
| Nevontaii Ltd | Wholesale & retail | 2020 |
| New Luce Community Trust | Other services | 2015 |
| Newton Stewart Cinema Limited | Information & communication | 1995 |
| Newton Stewart Hairdressers Ltd. | Other services | 2003 |
| Old Luce Community Association | Other services | 2026 |
| Old Luce Development Trust | Professional & scientific | 2016 |
| Olejaz Krest Consult Limited | Professional & scientific | 2026 |
| Optimum Heating and Renewables Limited | Construction | 2021 |
| Orca Fishing Company Limited | Agriculture & fishing | 2018 |
| Outmoved LP | | 2022 |
| Parker's Gym Ltd | Arts & recreation | 2026 |
| Peace Advocate Project Ltd | Education | 2018 |
| Penninghame Foundation | Health & social care | 2008 |
| Phoenix Lifeskills Nurturing Potential Limited | Education | 2025 |
| Phoenix Solar Energy Limited | Construction | 2024 |
| Ping 168 Ltd | Hospitality | 2017 |
| Pironi Construction Limited | Construction | 2012 |