| 2DM Drum Limited | Energy | 2011 |
| 3S Digital Media LP | | 2022 |
| 42 Albert Street (Newton Stewart) Limited | Professional & scientific | 2012 |
| A & a Robinson Limited | Agriculture & fishing | 2014 |
| A B & a Matthews LLP | | 2005 |
| A Polly for Every Pocket Limited | Wholesale & retail | 2024 |
| A. Lochhead Fishing Company Limited | Agriculture & fishing | 2007 |
| ABC Leisure (Machines) Limited | Arts & recreation | 2003 |
| Active Travel Design Ltd | Professional & scientific | 2024 |
| Alastair Paterson Limited | Financial services | 2025 |
| ALL Roads Lead to Whithorn | Real estate | 2014 |
| Alticry Consulting Limited | Information & communication | 2013 |
| Amaca Limited | Information & communication | 2019 |
| Andrew Plunkett Steel Buildings Limited | Manufacturing | 2000 |
| Artfield Farms | | 1982 |
| Ashby Property Ltd | Real estate | 2022 |
| Asheiri CIC | Health & social care | 2024 |
| ATD Electrical Contractors Limited | Construction | 2019 |
| Atlant Chemical Projects LP | | 2017 |
| Attema Ltd | Professional & scientific | 2017 |
| B Marshall Motor Engineer Ltd | Wholesale & retail | 2021 |
| Bairds Properties Limited | Real estate | 2008 |
| Bairds Windows Limited | Manufacturing | 2006 |
| Balfern Holdings Limited | Construction | 2016 |
| Bantech Ltd. | Manufacturing | 2013 |
| Barberras Botanicals Ltd | Wholesale & retail | 2024 |
| Barholm Enterprise Limited | Real estate | 2017 |
| Barnkirk Ltd | Agriculture & fishing | 2014 |
| Bents Farming Partnership | | 1998 |
| Biosphere Enterprises Ltd | Professional & scientific | 2023 |
| Bold Competitions Ltd | Arts & recreation | 2025 |
| Breakwater Land and Energy Limited | Agriculture & fishing | 2007 |
| Bubble FLO Ltd | Health & social care | 2016 |
| Building Futures Galloway (Training) Limited | Construction | 2022 |
| Building Futures Galloway Limited | Construction | 2022 |
| Cadel Limited | Hospitality | 2003 |
| Carsegowan Acres Ltd | Agriculture & fishing | 2025 |
| Castlewigg Gwynne Limited | Extraterritorial | 2014 |
| Causewayend Plant Nursery Ltd | Wholesale & retail | 2022 |
| Celsteel Limited | Wholesale & retail | 1963 |
| Central Bar (Newton Stewart) Limited | Hospitality | 2021 |
| Chikara Electrical Limited | Construction | 2024 |
| Cinnamon 2007 Limited | Professional & scientific | 2006 |
| CJM Trading Limited | Professional & scientific | 2023 |
| CK Energy Management Services Ltd | Construction | 2020 |
| Claxo Ltd | Manufacturing | 2020 |
| Claycrop Farms Ltd | Agriculture & fishing | 2016 |
| Cliffords Casualty Service Limited | Agriculture & fishing | 2009 |
| Connell Farm Ltd | Agriculture & fishing | 2016 |
| Connicks (UK) Ltd | Professional & scientific | 2012 |
| Conreb Ltd | Construction | 2018 |
| Coopon Carse Ltd. | Agriculture & fishing | 2025 |
| Crafty Scottish Distillers Ltd | Manufacturing | 2015 |
| Creative Rural Art Workshops CIC | Arts & recreation | 2025 |
| Cree Foods Limited | Extraterritorial | 2002 |
| Cree Valley Benefit Trust Limited | Other services | 2020 |
| Creetown Building Preservation Trust Limited | Other services | 2008 |
| Crosbie Property Solutions Ltd | Real estate | 2021 |
| Crossroads Care and Support | Other services | 2001 |
| Crown Glenluce Limited | Hospitality | 2023 |
| Cumloden Manor Nursing Home Limited | Health & social care | 2002 |
| Curly Tale Books Ltd | Information & communication | 2013 |
| D Millar Properties Ltd | Real estate | 2022 |
| D R Box Services Ltd | Wholesale & retail | 2022 |
| Dark Sky Park Limited | Hospitality | 2009 |
| Davidson Tractors Limited | Wholesale & retail | 2002 |
| Denta Art Limited | Health & social care | 2015 |
| Derry Farming Company | | 2002 |
| Document Records Limited | Information & communication | 2000 |
| Douglas Engineers Limited | Manufacturing | 2011 |
| Drama Kleenz Ltd | Administrative & support | 2024 |
| Driftwood Cinema C.I.C. | Arts & recreation | 2015 |
| Dumfries and Galloway Outdoor and Woodland Learning Group (Dgowl) CIC | Education | 2023 |
| Duncan Park Aerospace Limited | Manufacturing | 2004 |
| Dusty's Exterior Cleaning Ltd | Administrative & support | 2023 |
| Duthie Investments Ltd | Real estate | 2023 |
| EAT South Scotland CIC | Other services | 2026 |
| Elrig Eggs Limited | Agriculture & fishing | 2015 |
| Elrig Estate Company Limited | Financial services | 1991 |
| Elrig Property Limited | Real estate | 2023 |
| Estella Investments LP | | 2016 |
| Euan Mcconnell Ltd | Professional & scientific | 2006 |
| Evans Contracting Limited | Agriculture & fishing | 2011 |
| Farm Carbon Ltd | Extraterritorial | 2021 |
| Fleetbank House Ltd | Real estate | 2022 |
| Forest Excavations Limited | Agriculture & fishing | 1996 |
| Forgebay Ltd | Information & communication | 2026 |
| Forsyth Farms | Agriculture & fishing | 2002 |
| Friends of Calgow Limited | Education | 2023 |
| Fuller Three Limited | Manufacturing | 2022 |
| Gallery Art Marketing Ltd | Manufacturing | 2014 |
| Galloway & Ayrshire Partnership LLP | | 2011 |
| Galloway & Ayrshire Properties Ltd | Real estate | 2006 |
| Galloway Carpet Centre Limited | Wholesale & retail | 2016 |
| Galloway Fisheries Trust | Agriculture & fishing | 2015 |
| Galloway House Enterprises Limited | Real estate | 2019 |
| Galloway House Estate Courses Limited | Hospitality | 2025 |
| Galloway House Estate Ltd | Arts & recreation | 2025 |
| Galloway Nature and Heritage Trust | Professional & scientific | 2006 |
| Galloway Signs Ltd | Manufacturing | 2012 |