| DBJ Land & Property Investments Ltd | Construction | 2026 |
| DBS Fabrication Ltd | Professional & scientific | 2024 |
| DDB Property 7 Limited | Real estate | 2003 |
| DDS Health and Wellness Clinic Ltd | Health & social care | 2025 |
| Deaf Empowering Network | Health & social care | 2021 |
| Dean Watson Installations Limited | Construction | 2015 |
| DEE Cares Group Ltd | Wholesale & retail | 2026 |
| Denbeth Developments Ltd | Real estate | 2023 |
| Denebridge Limited | Real estate | 2016 |
| Denerco (UK) Limited | Extraterritorial | 2015 |
| Design Draughting Services Ltd | Professional & scientific | 2008 |
| Design Property Ltd | Financial services | 2016 |
| Devere Building Services Limited | Construction | 2009 |
| DEZINE7 Ltd | Manufacturing | 2022 |
| DF Building Services (NE) Ltd | Construction | 2019 |
| DGJ Consultancy Ltd | Energy | 2021 |
| DGL Images Ltd | Professional & scientific | 2015 |
| DGS PUB Company Limited | Hospitality | 2025 |
| Dialabiz Ltd | Professional & scientific | 2021 |
| Diggers 2U Limited | Construction | 2021 |
| Digital Edge Online Solutions Limited | Information & communication | 2019 |
| Direct Management Consulting Ltd | Administrative & support | 2014 |
| Dirty Bandit Virtual Golf Limited | Arts & recreation | 2024 |
| DIY Plastics Ltd | Extraterritorial | 2006 |
| Djam Consulting Ltd | Information & communication | 2020 |
| DJK Architecture Limited | Professional & scientific | 2019 |
| DJR Group Ltd | Wholesale & retail | 2025 |
| DK Rope Access Solutions Limited | Professional & scientific | 2013 |
| DKV Trans Ltd | Transport & logistics | 2019 |
| DLR Property Investments Limited | Real estate | 2023 |
| DLS Plumbing & Heating (NE) Ltd | Construction | 2020 |
| Dmac Holdings Ltd | Professional & scientific | 2017 |
| Dobson Asset Management Limited | Real estate | 2026 |
| Dobson Owens Security Consultants Ltd | Administrative & support | 2016 |
| Doddys International Ltd | Wholesale & retail | 2023 |
| Donotam Ltd | Wholesale & retail | 2025 |
| Doriflon Limited | Manufacturing | 1978 |
| DOT Gregory Ltd | Construction | 2025 |
| Dotnetpages Ltd | Information & communication | 2011 |
| Double D Couriers Ltd | Transport & logistics | 2024 |
| Dovetail Property Consultants Limited | Professional & scientific | 2016 |
| DPE Commercial Properties Ltd | Real estate | 2015 |
| Draper Homes Ltd | Real estate | 2022 |
| Ds-R Property NE Limited | Real estate | 2025 |
| Dueltech Limited | Information & communication | 2022 |
| Dunlavey Holdings Limited | Financial services | 2026 |
| Durham Autoclaves Limited | Administrative & support | 2003 |
| Durham Caravans Limited | Manufacturing | 2018 |
| Durham City Paint & Wallpaper Limited | Wholesale & retail | 2011 |
| Durham Design Limited | Wholesale & retail | 2015 |
| Durham Electrical Ltd | Manufacturing | 2024 |
| Durham Holdings Ltd | Real estate | 2018 |
| Durham Leather Ltd | Manufacturing | 2021 |
| Durham Precision Engineering Limited | Manufacturing | 2007 |
| Durham Precision Tea Machines Ltd | Manufacturing | 2024 |
| DWS Engineering Services (NE) Limited | Professional & scientific | 2016 |
| E & M Golf Limited | Wholesale & retail | 2005 |
| E-Koorb Ltd | Professional & scientific | 2022 |
| E.S. Property & Estate Ltd | Construction | 2018 |
| East Coast Comms Limited | Construction | 2025 |
| Ebac Heat Pumps Ltd | Energy | 2025 |
| Ebac Holdings Limited | Manufacturing | 2011 |
| Ecogrow Gardens Limited | Administrative & support | 2025 |
| Eden Inc Ltd | Information & communication | 2020 |
| Educate Fitness & Adventure Limited | Education | 2023 |
| Efpl EOT Limited | Extraterritorial | 2021 |
| Eggleston Hall Properties Limited | Real estate | 2019 |
| Eggleston Holdings Limited | Financial services | 2019 |
| EHL UK Group FM Ltd | Professional & scientific | 2023 |
| EHL UK Investments Ltd | Financial services | 2020 |
| Ehliq Ltd | Information & communication | 2023 |
| Ehluk Building Maintenance & Facility Services Ltd | Construction | 2001 |
| Ehluk Leisure Ltd | Hospitality | 2014 |
| EJS Electrical (North East) Limited | Construction | 2020 |
| Eldon Financial Planning Limited | Financial services | 2002 |
| Eldreich Limited | Manufacturing | 2026 |
| Elements Agility Ltd | Other services | 2026 |
| Elgey Ltd | Information & communication | 2022 |
| Elite Caravan Services Limited | Administrative & support | 2007 |
| Elite Care and Support Ltd | Information & communication | 2022 |
| Elite Sheet Ltd | Construction | 2019 |
| Elliotts Roofing NE Limited | Construction | 2026 |
| ELM Tech UK Ltd | Construction | 2006 |
| Embarc 2 Limited | Hospitality | 2021 |
| EMD Servicing Ltd | Manufacturing | 2024 |
| Emerald Forest Stevenage Limited | Real estate | 2025 |
| Emtec (Medical) UK Ltd | Education | 2011 |
| Engineered Space Ltd | Professional & scientific | 2023 |
| Enhanced by Nature Ltd | Wholesale & retail | 2020 |
| Envirogasservices Ltd | Wholesale & retail | 2023 |
| Environment Remediation Limited | Water & waste | 2019 |
| Envizage I.T. Limited | Information & communication | 2006 |
| Eric HR Limited | Administrative & support | 2017 |
| Essence Hair NE Limited | Other services | 2016 |
| Estheva Clinic Ltd | Other services | 2020 |
| Eternal Warmth Care Ltd | Health & social care | 2025 |
| Etex Holding UK Remagin Limited | Manufacturing | 2013 |
| EVA Emporium Ltd | Wholesale & retail | 2024 |
| Evando Trading Limited | Other services | 2019 |
| Evarine Ltd | Professional & scientific | 2017 |